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Three-dimensional large-aperture nanoscale fibrous scaffold and method for preparing same

A fibrous scaffold, large pore size technology, applied in scaffolds, medical science, blood vessels, etc., can solve the problems of affecting cell adhesion and growth, insufficient cell affinity, low adhesion, etc., and achieve good cell adhesion and good biological phase. Capability and tissue regeneration ability, the effect of less infection

Inactive Publication Date: 2012-12-12
冯淑芹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thicker cells of conventional biodegradable polyester fibers have lower adhesion and insufficient cell affinity, which affects the adhesion and growth of cells on its surface.

Method used

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  • Three-dimensional large-aperture nanoscale fibrous scaffold and method for preparing same
  • Three-dimensional large-aperture nanoscale fibrous scaffold and method for preparing same
  • Three-dimensional large-aperture nanoscale fibrous scaffold and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of fiber mat

[0026]

[0027] Electrospinning parameter control

[0028]

[0029] The purified PCL and PVP polymers with a weight fraction of 10% are dissolved in an organic solvent to obtain a transparent and uniform organic solution containing 5% by weight of the purified PCL polymer and 5% by weight of PVP. That is, the electrospinning stock solution.

[0030] Electrospinning was carried out with the prepared electrospinning stock solution. The spinning parameters were controlled at a voltage of 15kv, a nozzle aperture of 0.4mm, a solution flow rate of 1ml / h, an ambient temperature of 20°C, and a distance of 15cm between the receiving plate and the nozzle.

[0031] (2) Preparation of macroporous nanofiber scaffolds

[0032] Within 48 hours after spinning, the fiber mat was immersed in the organic solvent ethanol, pre-frozen at -200°C for 8 hours, and then frozen at -4°C, using CO 2 The atmosphere was dried for 24 hours, and a three-dimensio...

Embodiment 2

[0034] (1) Preparation of fiber mat

[0035]

[0036] Electrospinning parameter control

[0037]

[0038] The purified PCL and PVP polymers with a weight fraction of 40% were dissolved in an organic solvent to obtain a transparent and uniform organic solution containing 4% by weight of the purified PCL polymer and 36% by weight of PVP. That is, the electrospinning stock solution.

[0039] Electrospinning was carried out with the prepared electrospinning stock solution. The spinning parameters were controlled at a voltage of 20kv, a nozzle aperture of 0.7mm, a solution flow rate of 2ml / h, an ambient temperature of 25°C, and a distance between the receiving plate and the nozzle of 10cm.

[0040] (2) Preparation of macroporous nanofiber scaffolds

[0041] Within 48 hours after spinning, the fiber mat was immersed in the organic solvent acetone, pre-frozen at -4°C for 8 hours, and then frozen at -40°C, using CO 2 The atmosphere was dried for 12 hours, and a three-dimensi...

Embodiment 3

[0043] (1) Preparation of fiber mat

[0044]

[0045] Electrospinning parameter control

[0046]

[0047]The purified PLGA and PEO polymers with a weight fraction of 30% were dissolved in an organic solvent to obtain a transparent and uniform organic solution containing 9% by weight of the purified PLGA polymer and 21% by weight of PVP. That is, the electrospinning stock solution. Electrospinning was carried out with the prepared electrospinning stock solution. The spinning parameters were controlled at a voltage of 30kv, a nozzle aperture of 0.1mm, a solution flow rate of 0.2ml / h, an ambient temperature of 40°C, and a distance of 2cm between the receiving plate and the nozzle.

[0048] (2) Preparation of macroporous nanofiber scaffolds

[0049] Within 48 hours after spinning, immerse the fiber mat in a mixed solvent of organic solvent methanol and water, pre-freeze at minus 100°C for 1 hour, then freeze at 4°C, and apply CO 2 The atmosphere was dried for 48 hours, a...

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Abstract

The invention discloses a three-dimensional large-aperture nanoscale fibrous scaffold and a method for preparing the same. The average diameter of fibers in the scaffold is about 1 nanometer to 900 nanometers, the voidage is about 50%-98%, the scaffold is of a three-dimensional structure, and the thickness of the scaffold is 0.01 micrometer to 10 centimeters. The method includes the following steps of dissolving biodegradable polyester and additives into organic solvent to prepare spinning solution. An electrostatic spinning process is used for spinning the spinning solution to obtain nanoscale / submicron-order fibrofelt. The fibrofelt is soaked in solvent within 48 hours after being spun, is pre-frozen at first, and then is frozen and dried, so that the three-dimensional fibrous scaffold with mutually communicated large holes is obtained. The three-dimensional tissue engineering scaffold material with the communicated large holes is prepared, the structures of the internal holes are uniform, and the three-dimensional large-aperture nanscale fibrous scaffold can be used as a tissue engineering cytoskeleton for bones, cartilages, blood vessels, hearts, nerves and the like, and is suitable for growth of various cells.

Description

technical field [0001] The invention relates to a three-dimensional large-aperture nanoscale fiber scaffold and a preparation method, in particular to a method for preparing a scaffold for repairing defective tissue or constructing an organ by electrospinning technology, and belongs to the technical field of medical material manufacturing methods. Background technique [0002] How to construct tissue engineering repair materials with bionic natural extracellular matrix structure and function, so as to provide seed cells with a good growth, value-added and functional expression environment, and even induce and affect cell differentiation, has always been a difficulty and hot spot in tissue engineering research. At present, nano-tissue engineering scaffolds prepared by electrospinning can imitate the natural extracellular matrix at the nanoscale due to their large specific surface area, high porosity, and high surface energy, and can be used as porous scaffolds for cell growth....

Claims

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Application Information

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IPC IPC(8): A61F2/02A61F2/06A61F2/18A61F2/82A61F2/28A61L27/18A61L27/56A61L31/06
Inventor 冯淑芹
Owner 冯淑芹
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