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Production process for chondroitin sulfate from yak bone

A chondroitin sulfate and yak bone technology, applied in the field of biochemical pharmacy, can solve the problems of chondroitin sulfate production stop, difficult to treat waste water, environmental pollution, etc., and achieve the effects of reducing pollution, improving product quality and simple operation

Active Publication Date: 2013-01-02
西藏天虹科技股份有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic enterprises generally use strong alkali or dilute alkali to destroy protein, and then add acid hydrolysis and high-salt precipitation to remove protein. A large amount of protein, acid-base and high-salt are discharged with production wastewater, causing serious environmental pollution.
According to estimates, for every ton of chondroitin sulfate produced, wastes such as 5-10 tons of protein and 5-9 tons of acid-base salts will be discharged, so that the production of chondroitin sulfate will bring greater economic benefits and cause However, with the existing sewage treatment technology, it is difficult to treat such high-concentration wastewater even with huge investment. Therefore, the production of chondroitin sulfate has basically stopped in developed countries.

Method used

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  • Production process for chondroitin sulfate from yak bone
  • Production process for chondroitin sulfate from yak bone

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Take 10kg of fresh yak throat, wash it, put it into a cooking pot with constant temperature at 80°C, add water to submerge it, cook for 3 hours, remove it, remove impurities such as wrapping film and fibrous hard bone tissue on the throat, and get pure throat. Freeze at -20°C. When ready to use, dry it at 70°C for 3 hours, crush the yak cartilage with a pulverizer and put it into the reaction kettle, add an appropriate amount of water, and use alkaline trypsin and acid pepsin to treat the above Sufficient hydrolysis of yak cartilage: adjust the pH value to 8.5 with 2mol / L HCI, press 1000:0.5 (V 滤液 :V 胰酶 ) was added to trypsin, and hydrolyzed at 45°C for 1 hour. Then add pepsin at a ratio of 1000:0.5, and hydrolyze at 45°C for 1.5 hours; after the enzymolysis solution is cooled, filter it with a 500-mesh filter cloth, and then use a MNWC-5000 hollow fiber ultrafilter to concentrate about half of the volume to obtain concentrated filtered enzyme Solution. Add 95% alco...

Embodiment 2

[0023] Take 200kg of fresh yak soft ribs, wash them, put them into a 80°C constant temperature cooking pot, add water to submerge, cook for 4 hours, remove them, remove impurities such as the wrapping film and fibrous hard bone tissue on the throat bone, and get pure soft ribs, put them in the cold storage- Quick-frozen at 20°C; dry at 80°C for 4 hours when in use, crush the yak cartilage with a pulverizer for later use; put the white and clean yak throat bone into the reaction kettle, add appropriate amount of water, and use alkaline trypsin and acid pepsin to treat the cartilage The above-mentioned yak cartilage is fully enzymatically hydrolyzed: adjust the pH value to 8.6 with 2mol / L HCI, press 1000:0.5 (V 滤液 :V 胰酶 ) at a ratio of 50°C for 1 hour; add pepsin at a ratio of 1000:0.5, and hydrolyze for 1.5 hours at 50°C; Concentrate half the volume of the filter to obtain the concentrated filtered enzymolysis solution; add 95% alcohol (food grade) to the obtained filtrate und...

Embodiment 3

[0028] Take 200kg of fresh yak soft ribs, wash them, put them in a cooking pot with a constant temperature of 80°C, add water to submerge them, cook for 4 hours, remove them, remove impurities such as the wrapping film and fibrous hard bone tissue on the throat bone, and get pure soft ribs, put them in the cold storage- Quick-frozen at 20°C. When ready to use, dry it at 80°C for 4 hours, and crush the yak cartilage with a pulverizer before use. Put the white and clean yak throat bone into the reaction kettle, add appropriate amount of water, and use alkaline protease trypsin and acid protease pepsin to fully hydrolyze the above-mentioned yak cartilage: adjust the pH value to 8.6 with 2mol / L HCI, press the filtrate Add trypsin at a ratio of 1000:0.5 to trypsin, and hydrolyze at 50°C for 1 hour. Then add pepsin at a ratio of 1000:0.5, and hydrolyze at 50°C for 1.5h. After the enzymatic hydrolysis solution is cooled, filter it with a 500-mesh filter cloth, and then use a MNWC-5...

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Abstract

The invention relates to a production process for a chondroitin sulfate from yak bone. The process includes using waste nose bone, larynx bone, soft costa, trachea, crescent moon bone, and other cartilages of Qinghai-Tibet Plateau unique species yak as raw materials, and preparing chondroitin sulfate via two-enzyme enzymolysis method, precipitation separation method, filtration, ethanol precipitation, vacuum drying, etc. The invention solves the problem of complex process flow, severe contamination, large consumption of alkali, acid, or salt, long extraction period, and low yield of this kind of products existing in the prior art. Chondroitin sulfate content achieves over 90%, and chondroitin sulfate yield achieves over 18%. Simultaneously, full use of yak waste raw material alleviates environmental contamination, effectively enhances utilization of resources, and facilitates commercial process.

Description

technical field [0001] The invention relates to a production process for preparing chondroitin sulfate from yak bone. Specifically, the present invention relates to the preparation of sulfuric acid by adopting double enzymatic hydrolysis, filtration, ethanol precipitation, vacuum drying and other cartilage discarded after slaughtering yak, a characteristic species of the Qinghai-Tibet Plateau, as raw materials Chondroitin belongs to the technical field of biochemical pharmacy. Background technique [0002] Chondroitin Sulfate (Chondroitin Sulfate, ChS) is one of the typical polysaccharide drug varieties, with a long history of clinical application, and the scope of application continues to expand. In addition to the obvious effect on the prevention and treatment of osteoarthritis, the compound preparation of chondroitin sulfate is effective in neuralgia. It is also widely used in the treatment of senile shoulder pain and skin diseases. With the advancement of science and t...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 高聚霞
Owner 西藏天虹科技股份有限责任公司
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