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Hemoglobin microcapsule blood substitute and its preparation method

A hemoglobin and microcapsule technology, applied in microcapsules, blood diseases, capsule delivery, etc., can solve problems such as difficulty in obtaining controllable particle size, structure and function, large particle size of microcapsules, and difficulty in oxygen release.

Active Publication Date: 2013-01-16
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the process is simple, the particle size of the prepared microcapsules is too large, and the oxygen affinity is too strong, so it is not easy to release oxygen
[0009] The above research results show that it is difficult to obtain hemoglobin blood substitutes with adjustable particle size, structure and function, and good biocompatibility and hemocompatibility

Method used

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  • Hemoglobin microcapsule blood substitute and its preparation method
  • Hemoglobin microcapsule blood substitute and its preparation method
  • Hemoglobin microcapsule blood substitute and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Preparation of oxidized heparin (DHP) with a dialdehyde structure

[0046] 0.6 g of heparin was prepared into an aqueous solution with a mass concentration of 1% with distilled water, 0.65 g of sodium periodate was added thereto, and an appropriate amount of ethylene glycol was added to terminate the reaction after 24 hours of dark reaction. After mixing the solution with 0.5g sodium chloride, use 150mL of absolute ethanol to precipitate the product, filter with suction and dissolve the precipitate with deionized water, precipitate with ethanol again, filter with suction, repeat this step 3 times, put the product into a watch glass, 50 ℃ vacuum drying for 24h, you can prepare DHP.

[0047] (2) Biocompatible hemoglobin microcapsules assembled based on Schiffer base bonds

[0048] First, disperse manganese carbonate particles with a particle size of 4 μm into an acetate buffer solution (pH=5) of 4 mg / mL bovine hemoglobin (Hb) containing 0.1 M sodium chloride, shake a...

Embodiment 2

[0056] (1) Preparation of oxidized sodium alginate (alginate dialdehyde, ADA) with a dialdehyde structure

[0057] 4 g of sodium alginate was prepared with distilled water into an aqueous solution with a mass concentration of 2.5%, 4.5 g of sodium periodate was added thereto, and an appropriate amount of ethylene glycol was added to terminate the reaction after 24 hours of dark reaction. After mixing the solution with 10g of sodium chloride, use 200mL of absolute ethanol to precipitate the product, filter with suction and dissolve the precipitate with deionized water, precipitate with ethanol again, filter with suction, repeat this step 3 times, put the product into a watch glass, and keep at 40°C Vacuum drying for 24h, ADA can be prepared.

[0058] (2) Biocompatible hemoglobin microcapsules assembled based on Schiffer base bonds

[0059] First, disperse silica particles with a particle size of 200nm into an acetate buffer solution (pH=5) of 4 mg / mL sheep hemoglobin (Hb) cont...

Embodiment 3

[0061] (1) Preparation of oxidized cellulose (diaaldehyde cellulose, DAC) with a dialdehyde structure

[0062] 3 g of cellulose was prepared into a solution with a mass concentration of 2.5% with distilled water, 3.2 g of sodium periodate was added thereto, and an appropriate amount of ethylene glycol was added to terminate the reaction after 24 hours of dark reaction. After mixing the solution with 8g sodium chloride, use 200mL absolute ethanol to precipitate the product, filter with suction and dissolve the precipitate with deionized water, precipitate with ethanol again, filter with suction, repeat this step 3 times, put the product into a watch glass, and keep at 40°C DAC can be prepared by vacuum drying for 24h.

[0063] (2) Biocompatible microcapsules assembled based on Schiffer base bonds

[0064]First, disperse polystyrene colloidal particles with a particle size of 600nm into an acetate buffer solution (pH=5) of 5 mg / mL bovine hemoglobin (Hb) containing 0.5M sodium c...

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Abstract

The invention discloses a hemoglobin microcapsule and its preparation method. The hemoglobin microcapsule is a biocompatible and blood-compatible hemoglobin microcapsule blood substitute prepared through Schiff base bond layer-by-layer assembling, the capsule wall components used by the hemoglobin microcapsule comprise a biocompatible and biodegradable polysaccharide derivative and hemoglobin, Schiff base bonds are formed between the components of the capsule wall, and a toxic crosslinking agent is not used in the invention. The hemoglobin microcapsule blood substitute obtained in the invention has the advantages of good stability, biological compatibility, blood compatibility, biodegradability, non-toxicity, no use of the crosslinking agent, and adjustable dimension, structure and functions.

Description

technical field [0001] The invention relates to a hemoglobin microcapsule blood substitute and a preparation method thereof. Background technique [0002] Currently, blood transfusion has become an important part of medical care. Clinical blood transfusion technology is safe, but due to the serious shortage of clinical blood stocks, the need for type matching between blood donors and recipients, and blood cross-infection, the wide application of ligand blood transfusion technology in emergency situations is limited. Therefore, seeking safety Effective blood substitutes are of great significance to transfusion medicine. [0003] The research on blood substitutes is mainly carried out in two directions: fluorocarbons and hemoglobin (Hb) products. Fluorocarbons play a role through the high solubility of oxygen, can effectively carry oxygen and release oxygen, and maintain blood volume and electrolyte balance. However, the storage conditions of fluorocarbons are special, they...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K38/42A61K47/36A61K47/38A61M16/10A62D9/00A61P7/06A61P7/08
Inventor 李峻柏贾怡崔岳费进波杨洋
Owner INST OF CHEM CHINESE ACAD OF SCI
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