Preparation method and application of macroporous penicillin acylase immobilized magnetic bead polymer carrier

Abstract

The invention relates to a preparation method of a polymer carrier, particularly a preparation method of a macroporous penicillin acylase immobilized magnetic bead polymer carrier, which comprises the following steps: dissolving Fe<2+> and Fe<3+> salts used as magnetic source precursors in a polar organic solvent formamide while introducing nitrogen, regulating the pH value of the solution to 8-11 with concentrated ammonia water, curing at 60-90 DEG C for 30-60 minutes, and forming a magnetic fluid under alkaline conditions; by using a surfactant as a dispersant, a vinyl compound as a monomer and a nonpolar organic substance as a dispersion medium, carrying out cross-linking polymerization under the action of an initiator by an reversed phase suspension technique; and washing with acetone to separate the product in a magnetic field, washing with an organic solvent, and carrying out vacuum drying at room temperature to obtain the carrier. The invention has the advantages of unique method, low synthesis cost and simple process, omits the processes of Fe3O4 preparation and surface modification, is beneficial to industrialized application, and can obtain the magnetic macroporous bead penicillin acylase immobilized carrier. The carrier is used for preparing high-activity segregative immobilized enzymes by penicillin acylase immobilization.

Description

technical field

[0001] The invention relates to a preparation method of a polymer carrier, in particular to a preparation method of a macroporous penicillin acylase-immobilized magnetic bead polymer carrier and its application. Background technique

[0002] Penicillin acylase, or PGA, is an industrial enzyme for the production of semi-synthetic β-lactam antibiotics. Its important application is to catalyze the key intermediate 6-aminopenicillane required for the production of semi-synthetic β-lactam antibiotics from penicillin G. The acid is 6-APA. At present, nearly 90% of 6-APA in the world is produced by penicillin acylase biocatalyst immobilized on natural polymer or synthetic resin carrier. Although immobilized PGA has been used in the pharmaceutical industry for nearly three decades, the preparation of new immobilized PGA carriers suitable for industrial applications is still challenging. The preparation of immobilized enzyme by magnetic carrier, the separation, wash...