Method for production of citric acid through fermentation of wheat B-starch

A technology of B starch and citric acid, which is applied in the field of fermentation to produce citric acid, can solve the problems of high cost and low output, and achieve the effects of reducing grain consumption, reducing viscosity, and improving fermentation conversion rate

Inactive Publication Date: 2013-01-30
RIZHAO LUXIN JINHE BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, with the further spread of the food crisis, the supply of corn as the main food has become increasingly tense, and cassava and dried melons have been limited in their wide application due to factors s

Method used

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  • Method for production of citric acid through fermentation of wheat B-starch

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Embodiment 1

[0031] A method for fermenting wheat B starch to produce citric acid, comprising pulping, liquefaction, saccharification, and fermentation, characterized in that: its specific steps are:

[0032] (1) Measure 70 tons of wheat B starch on a dry basis, add water to prepare a B starch slurry with a mass fraction of 25%;

[0033] (2) Add 35kg of high-temperature α-amylase to the B starch slurry, and input it into the high-temperature jet liquefaction system for liquefaction to obtain the B starch slurry liquefaction solution; the liquefaction temperature is 110°C, and the time is 0.5h;

[0034] (3) Cool down the B starch slurry liquefaction solution to 67°C, adjust the pH to 4.0, add 15kg cellulase, 20kg xylanase, 5kg lysophospholipase, and enzymatically hydrolyze for 5 hours to obtain an enzymolyzed solution;

[0035] (4) Add 50kg of acidic compound glucoamylase to the enzymolysis solution, and saccharify for 16 hours to obtain a saccharification solution;

[0036] (5) Filtration...

Embodiment 2

[0041] A method for fermenting wheat B starch to produce citric acid, comprising pulping, liquefaction, saccharification, and fermentation, characterized in that: its specific steps are:

[0042] (1) Measure 70 tons of wheat B starch slurry on a dry basis, add water to prepare the B starch slurry with a mass fraction of 27%;

[0043] (2) Add 42kg of high-temperature α-amylase to the B starch slurry, and input it into the high-temperature jet liquefaction system for liquefaction to obtain the B starch slurry liquefaction solution; the liquefaction temperature is 100°C, and the time is 1h;

[0044](3) Cool down the B starch slurry liquefaction solution to 70°C, adjust the pH to 4.2, add 7 kg of cellulase, 30 kg of xylanase, and 7 kg of lysophospholipase, and perform enzymatic hydrolysis for 10 hours to obtain an enzymatic hydrolysis solution;

[0045] (4) Add 70 kg of acidic compound glucoamylase to the enzymatic hydrolysis solution, and saccharify for 10 hours to obtain a sacch...

Embodiment 3

[0051] A method for fermenting wheat B starch to produce citric acid, comprising pulping, liquefaction, saccharification, and fermentation, characterized in that: its specific steps are:

[0052] (1) Measure 70 tons of wheat B starch slurry on a dry basis, add water to prepare the B starch slurry with a mass fraction of 30%;

[0053] (2) Add 25kg of high-temperature α-amylase to the B starch slurry, and input it into the high-temperature jet liquefaction system for liquefaction to obtain the liquefaction solution of B starch slurry; the liquefaction temperature is 95°C and the time is 2 hours;

[0054] (3) Cool down the B starch slurry liquefaction solution to 65°C, adjust the pH to 4.5, add 20kg of cellulase, 14kg of xylanase, and 3.5kg of lysophospholipase, and perform enzymatic hydrolysis for 7 hours to obtain an enzymatic hydrolysis solution;

[0055] (4) Add 60 kg of acidic compound glucoamylase to the enzymolysis solution, and saccharify for 12 hours to obtain a sacchari...

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Abstract

The invention relates to a method for production of citric acid through fermentation of wheat B-starch. The method is characterized in that the method improves a conventional citric acid fermentation process with corn as a raw material by using the by-product B-starch produced during processing of wheat to partially replace corn for fermentation production of citric acid and comprises the procedures of slurry mixing, liquefaction, enzymatic hydrolysis, saccharification, filtration and fermentation, and through full liquefaction, enzymatic hydrolysis and saccharification of the wheat B-starch and filtration, the method allows the viscosity of feed liquid to be greatly reduced while allowing the conversion rate of the starch to substantially reach a theoretical value and overcomes the problems of poor mass transfer and bad dissolution of oxygen in deep aerobic fermentation process of the wheat B-starch. On such an occasion that grain raw materials are in shortage, the process utilizes the cheap wheat B-starch, so production cost of citric acid is substantially reduced, and the source of raw materials for citric acid is broadened.

Description

technical field [0001] The invention relates to a method for fermenting and producing citric acid, which is characterized by fermenting and producing citric acid by using B starch, a by-product separated from wheat processing, as a raw material. Background technique [0002] At present, the domestic citric acid industry mainly uses corn, dried melon, cassava, etc. Miscellaneous, directly crushed into corn flour, mixed with water in a certain proportion, under the action of amylase, after high-temperature jet liquefaction treatment, it becomes a liquefied liquid with good fluidity and easy utilization by bacteria, and enters the fermentation process. During the fermentation process, Citric acid is accumulated through the metabolism of Aspergillus niger species, and after the fermentation is completed, the fermentation broth containing citric acid is extracted and refined downstream to obtain a citric acid product. Corn dregs and mycelium dregs are produced as by-products dur...

Claims

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Application Information

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IPC IPC(8): C12P7/48C12R1/685
Inventor 寇光智李昌涛周一江张玲玲高晓彤王晓飞
Owner RIZHAO LUXIN JINHE BIOCHEM
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