Compound with anti-herpes-virus function
An anti-herpes virus and herpes virus technology, applied in the field of compounds, can solve the problems of large side effects and application restrictions, and achieve the effects of low toxicity, low adverse reactions and wide application prospects
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Embodiment 1
[0020] The preparation of embodiment 1 new compound
[0021] Carry out solid fermentation of the croton to obtain the fermented croton, then reflux the fermented croton with 50-80% methanol to extract 3 times, each time for 2 hours, and concentrate the extract to obtain an extract, suspend the extract in water, and successively use 6 Double the volume of ethyl acetate and 6 times the volume of n-butanol for extraction to obtain the ethyl acetate site and n-butanol site, then use silica gel column chromatography with dichloromethane-methanol system as eluent, the resulting n-butanol Gradient elution at the butanol site (100:1, 100:5, 100:10, 100:20, 100:30, 100:40, 100:50), TLC tracking, combined dichloromethane-methanol (100:30, 100:40), elution liquid, concentrated, recrystallized and purified to obtain a new compound—crotontriazolidine (1-β-D-ribofuranose-6-amino-1,2,3-triazolo[5,4-d]pyridine ).
[0022] The compound is white needle crystal, slightly soluble in water, solu...
Embodiment 2
[0030] Embodiment 2 anti-herpes virus experiment
[0031] 1 Experimental method
[0032] 1.1 Compound Toxicity Test
[0033] Culture Vero cells in 10% calf serum RPMI-1640 medium, press 1×10 5 Inoculated into 96-well cell culture plates, 0.2 mL per well, grown into monolayer cells for 18-24 hours, discarded the supernatant, and set aside. The compound provided by the present invention (crotontriazolidine) was cultured for 72 hours in double-folded concentrations of the medium, 200 μL per well, and 4 duplicate wells for each concentration. Add 20 μL of 5 mg / ml MTT solution to each well, continue to incubate for 4 hours, pour off the supernatant, add 0.2 mL DMSO, measure O.D value with 570-630 nm dual-wavelength method, and calculate TC50 of the compound provided by the invention.
[0034] 1.2 CPE test and MTT test of compounds against HSV-I
[0035] vero cells by 1 x 10 5 Inoculated into 96-well cell culture plates, 0.2 mL per well, grown into monolayer cells for 18-24 hou...
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