Method for extracting cyanidin from black bean peel

A technology of anthocyanins and black soybean hulls, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of long purification time, large elution volume, large resin feed volume, etc., and achieve good chemical stability , High separation efficiency, pollution control effect

Active Publication Date: 2013-02-27
JIANGSU JIUWU HITECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN1431205A discloses an extraction agent and extraction method for extracting proanthocyanidins from plants. Water is selected as the extraction agent. The extraction takes a long time, the temperature is high, and there are many dissolved impurities, and the loss rate of anthocyanins is high.
Invention patent CN102260587A discloses a method for extracting grape seed oil and proanthocyanidins, using an organic ultrafiltration membrane to purify the extract to obtain anthocyanins. This method does not use a pretreatment method to control the material entering the organic ultrafiltration membrane, and the organic ultrafiltration membrane is seriously polluted ; In addition, it is difficult to maintain high flux after organic membrane fouling
Application number CN101525325A discloses a method for extracting anthocyanins from lingonberry, which includes steps such as crushing, extraction, filtration, and resin purification. The use of inorganic membranes effectively solves the problems of serious pollution of organic membranes and difficulty in recovering the flux. There are disadvantages such as large amount of resin feed, long purification time, large elution volume, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Weigh 100g of dry black soybean skin, wash it with water, extract with 40% ethanol solution, the mass of ethanol solution is 1kg, extract twice at a temperature of 40°C, each time for 50min, and combine the two extracts; the obtained extract The solution was centrifuged at 3000rpm / min for 10min to obtain the supernatant;

[0038] The obtained supernatant was filtered with a 100nm ceramic membrane, and the filter was controlled to operate under the operating conditions of a temperature of 30°C, a flow velocity of the membrane surface of 0.5m / s, and a pressure of 0.1MPa, and the permeate of the ceramic membrane was collected;

[0039]Concentrate the ceramic membrane permeate with a roll-type nanofiltration membrane with a molecular weight cut-off of 150. The concentration is operated under the operating conditions of a temperature of 30°C, a membrane surface flow rate of 1m / s, and a pressure of 0.5MPa. Collect the concentrate. The obtained nanofiltration membrane concent...

Embodiment 2

[0041] Weigh 100g of dry black soybean skin, wash it with clear water, and use pure water equivalent to 10 times the amount of black soybean skin to extract 3 times at a temperature of 50°C, each time for 100min, and combine the extracts for 3 times; Under the condition of 1 min, centrifuge for 10 min to obtain the supernatant;

[0042] Filter the obtained supernatant with a 20nm ceramic membrane, control the filter to operate under the operating conditions of a temperature of 50°C, a membrane surface velocity of 3m / s, and a pressure of 0.3MPa, and collect the permeate of the ceramic membrane;

[0043] Concentrate the ceramic membrane permeate with a roll-type nanofiltration membrane with a molecular weight cut-off of 300. The concentration is operated under the operating conditions of a temperature of 45°C, a membrane surface flow rate of 1m / s, and a pressure of 1.0MPa, and is collected after concentration. Concentrate. The obtained nanofiltration membrane concentrate was pu...

Embodiment 3

[0045] Weigh 100g of dry black soybean skins, wash them with water, and use dilute hydrochloric acid with a volume concentration of 0.5% equivalent to 20 times the amount of black soybean skins to extract at a temperature of 70°C for 100 minutes to obtain an extract; conditions, centrifuged for 10 min to obtain the supernatant;

[0046] The obtained supernatant was filtered with a 50nm ceramic membrane, and the filter was controlled to operate under the operating conditions of a temperature of 70°C, a flow velocity of the membrane surface of 4m / s, and a pressure of 0.5MPa, and the permeate of the ceramic membrane was collected;

[0047] Concentrate the ceramic membrane permeate with a roll-type nanofiltration membrane with a molecular weight cut-off of 200. The concentration is operated under the operating conditions of a temperature of 40°C, a membrane surface flow rate of 1m / s, and a pressure of 1.0MPa. After the concentration is completed, it is collected Concentrate. The ...

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Abstract

The invention relates to a method for extracting cyanidin from black bean peel, which comprises the following steps: A) after cleaning black bean peel, adding water and ethanol into the black bean peel to carry out extraction, thereby obtaining an extracting solution; B) carrying out centrifugal separation on the extracting solution to obtain a supernatant, carrying out trash extraction on the supernatant with a ceramic membrane to obtain a ceramic membrane permeation solution; C) concentrating the ceramic membrane permeation solution with an organic nanofiltration membrane to obtain a concentrated solution, and eluting the resin to obtain an eluate; and D) carrying out spray drying on the eluate to obtain the cyanidin. By adopting the centrifugal machine separation, membrane separation and resin integration technology, the invention can effectively lower the production cost, shorten the extraction separation time and enhance the product yield and product purity.

Description

technical field [0001] The invention relates to an extraction of anthocyanins, in particular to a method for extracting anthocyanins from black soybean hulls through a membrane integration separation and purification process, and belongs to the technical field of natural product extraction. Background technique [0002] Anthocyanins, also known as anthocyanins, are a class of water-soluble natural pigments widely present in plants, mostly in the form of glycosides, also known as anthocyanins. The earliest and most abundant anthocyanin is grape skin red extracted from red grape pomace, which was listed in Italy in 1879. The structural nucleus of anthocyanins is 22 phenylbenzopyran cations, which belong to flavonoid compounds. [0003] Anthocyanin (black soybean hull extract) is a polyphenolic substance, it is a new type of high-efficiency antioxidant, has the functions of promoting blood circulation, protecting eyesight, eliminating edema, decomposing and eliminating cholest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C09B61/00
Inventor 丁邦超姜海凤彭文博王贝贝寇琴熊福军
Owner JIANGSU JIUWU HITECH
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