Medical application of prze-watanshinquinone B

A technology of danshen and B, applied in the preparation of anti-tumor and anti-angiogenesis drugs, the field of danshen B, can solve problems such as no research report, achieve high efficiency, simple and reliable experimental method, and is conducive to popularization and application Effect

Inactive Publication Date: 2013-03-06
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Salvia miltiorrhiza isolated from Salvia ganxie is a diterpene quinone compound, and no relevant research on the activity of this compound has been found so far, so the research on the anti-tumor and angiogenesis inhibitory effects of this compound has not been reported.

Method used

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  • Medical application of prze-watanshinquinone B
  • Medical application of prze-watanshinquinone B
  • Medical application of prze-watanshinquinone B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Preparation of Purple Salvia B (Purple Salvia B used in the following examples was prepared by the following method)

[0022] 7.3 kg of dry sage (whole plant) was crushed through a 60-mesh sieve, ultrasonically extracted 5 times with 100% acetone (20 L each time) at room temperature, and the extract was concentrated under reduced pressure to obtain 300 g of crude extract; The obtained crude extract was put on a normal-phase silica gel (80-100 mesh, Qingdao Ocean Chemical) column (dry loading), followed by pure petroleum ether, petroleum ether-ethyl acetate mixture (petroleum ether: ethyl acetate is 9 :1, 3:1, 1:1, 3:7) for elution, collect each part of the eluate, concentrate under reduced pressure, transfer the concentrated solution to a 10 mL penicillin bottle separately, and use TLC for detection. Fractions were combined; petroleum ether: ethyl acetate=1:1 eluent elution fraction (52 g) was separated, and the separation method was normal phase silica gel c...

Embodiment 2

[0026] Example 2: In vitro anti-tumor effect experiment of purple salvia miltiorrhiza B - MTT experiment

[0027] According to the routine MTT experiment process, the logarithmic phase human liver cancer cell line HepG-2 was inoculated on a 96-well plate with an inoculation amount of about 3000 cells per well. After culturing at 37 °C for 24 h, six different concentrations ( 0.1, 0.5, 1.0, 2.5, 5.0, 10 mg / L) of Danshen B administration group and the blank control group, each concentration of drug was added to 5 wells, incubated at 37 ℃ for 72 h, and MTT (3 -(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide), 20 μL per well, continue to incubate for 4 h, discard the supernatant, add 150 μL DMSO to each well, shake After 1 h, the absorbance OD value was measured at 490 nm in a microplate reader. (See figure 2 )

[0028] The results showed that the proliferative activity of tumor cells decreased significantly when the concentration of salvia miltiorrhiza was 5.0 mg / L a...

Embodiment 3

[0030] Example 3: Anti-angiogenesis effect experiment of purple danshen B in vivo

[0031] Take fresh eggs at a temperature of 37.8°C, CO 2The concentration was 5%, incubated for 7 days under suitable humidity conditions, and then a window (1×1 cm) was opened at the end of the air chamber, and a sterile filter paper sheet with a diameter of 6 mm was used as the drug delivery carrier, and 40 μL of purple salvia miltiorrhiza B drug solution was dropped on the sterile filter paper, and the filter paper was evaporated to dryness, and then the filter paper was placed on the allantoic membrane of the chicken embryo, and three concentrations were set for the administration group (5 mg / L, 10 mg / L, L, 15 mg / L), and set up a blank control group (normal saline group) and a positive control drug group (dexamethasone group). After the operation, seal the window with sterile transparent tape, continue to incubate the eggs under the above conditions for 48 h, remove the allantoic membrane t...

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PUM

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Abstract

The invention discloses an application of compound prze-watanshinquinone B to preparing antitumor angiogenesis abnormal new drugs. An MTT experiment proves that the prze-watanshinquinone B can inhibit tumor cell proliferation, when drug concentration is 5.0-10.0mg/L, the cell proliferation activity is reduced remarkably; the chick embryo chorioallantoic membreme experiment proves that the prze-watanshinquinone B with the concentration of 10mg/L and 15mg/L can obviously inhibit angiogenesis of the chick embryo chorioallantoic membreme, so the prze-watanshinquinone B can be used for preparing the antitumor angiogenesis abnormal new drugs and has good curative effect and high safety, and is suitable for popularizing and using actually.

Description

technical field [0001] The invention belongs to the field of herbal medicinal ingredients and anti-angiogenesis compounds, in particular to the traditional Chinese medicine Salvia gansei ( Salvia przewalskii The use of purple salvianolin isolated from Maxim) in the preparation of anti-tumor and anti-angiogenesis drugs. Background technique [0002] In recent years, the morbidity and mortality of cancer have risen sharply, and it has become the number one killer threatening human health and life. [0003] The current cancer treatment methods mainly include: surgery, radiation therapy, and chemotherapy, but because of the limitations and lack of specificity of these treatment methods, the application of the above methods in cancer treatment is relatively limited. Therefore, finding a class of anti-tumor drugs that have a specific killing effect on cancer cells and have few side effects has become a research hotspot in the current medical field. In recent years, since the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61P35/00
Inventor 李宝才庄文婷朱路平李鹏向诚何静秦谊戴伟锋张敉
Owner KUNMING UNIV OF SCI & TECH
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