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Oil-reservoir endogenous microbe in-situ recovery method

A technology of endogenous microorganisms and microorganisms, which is applied in the direction of biochemical equipment and methods, measurement/inspection of microorganisms, etc., can solve problems such as temperature resistance lower than 80°C, inability to survive, restricting the understanding of extremophiles, and achieve standard operating procedures Flexible, flexible application methods, and short process duration

Active Publication Date: 2014-08-06
PETROCHINA CO LTD
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  • Claims
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AI Technical Summary

Problems solved by technology

Limited by the current microbial sampling technology and subsequent development methods, there are bottlenecks in the environmental adaptability of microorganisms that can be applied to microbial enhanced oil recovery. For example, the temperature resistance is generally lower than 80°C, and the specific unable to function or even survive in the reservoir environment, etc.
Analyzing the reasons, the possible influencing factors are: the sampling method of microbial research in the petroleum industry is to sample from the surface or oil-water wellhead; and the underground extremophiles experience the transportation process from the wellbore to the ground, the release of pressure and the change of temperature make some thermophilic , halophilic, and barophilic extremophiles die due to environmental changes, and their possible remaining genome resources are also difficult to extract and analyze through existing technical means due to environmental degradation and other issues
The above-mentioned technical bottlenecks in the process of collecting samples have seriously restricted people's understanding of extremophiles, and also hindered the development and application of endogenous microbial resources to a considerable extent.

Method used

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  • Oil-reservoir endogenous microbe in-situ recovery method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 Well Selection Basis and Parameters

[0029] Due to the different analysis purposes, the well selection principles of PVT sampling for endogenous microorganism research are slightly different from those of conventional PVT sampling. Conventional PVT sampling wells require the selection of oil and gas wells with a water production rate of less than 5%. PVT sampling for microbial research is to eliminate the interference of external microorganisms. It is required that the selected oil wells are oil wells in uninjected layers, or oil wells that have not been reinjected for more than 30 days after water injection, and there is no requirement for water production rate.

[0030] Through strict screening, it was determined that endogenous microbial PVT sampling was carried out in wells A and B in different blocks of a PetroChina oilfield. The basic reservoir conditions are shown in Table 1.

[0031] Table 1 Basic parameters of test wells

[0032]

Embodiment 2

[0033] Embodiment 2 Sampling steps

[0034] On-site operating equipment includes operating vehicles, PVT instruments, aseptic microbial collection systems on wells, equipment and instruments required for microbial screening, etc.

[0035] Set the sampling depth to 4500m, and the lowering speed to 100m / min; the PVT automatic opening and collection timing is 1 hour, and the collection time is 10 minutes; after the PVT completes the opening, collecting, and closing procedures, lift the cable to recover the PVT instrument; operate aseptically on the ground Quickly open the PVT to collect samples.

[0036] The endogenous microorganisms in the reservoir were sampled by PVT, and the wellhead oil samples of the corresponding wells were collected as a control. The sample collected by PVT is a light yellow liquid with an oil content of less than 10%; the wellhead sample is a dark brown oil-water mixture with an oil content of 40-50%.

[0037] From the observation of the apparent prope...

Embodiment 3

[0038] Example 3 extracts microbial DNA from collection samples

[0039] 1) Take about 100mL of crude oil and place it in a glass beaker, and put it in a water bath at 70°C for 30 minutes.

[0040] 2) Add an equal volume of 30% ethanol and a half volume of crude oil petroleum ether:n-hexane=1:1 mixture, and shake well.

[0041] 3) Stand still to separate the oil and water phases, and use a rubber hose to extract the lower water phase. Use filter paper to filter out a small amount of crude oil and other impurities in the aqueous phase.

[0042] 4) After filtration, the liquid is vacuum-filtered with a 0.22 μm microporous filter membrane, and bacteria are enriched on the filter membrane.

[0043] 5) Cut the filter membrane into pieces and put it into a 10 mL centrifuge tube, add 360 μL lysozyme (10 mg / mL dissolved in 10 mM Tris-HCl, pH 8.0), and bathe in water at 37° C. for 1 h.

[0044] 6) Add 115 μL of 10% SDS and 25 μL of proteinase K (20 mg / mL), and bathe in water at 37° ...

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Abstract

The invention relates to an oil-reservoir endogenous microbe in-situ recovery method. The method comprises the following steps: 1, well ore data analysis: collecting and organizing base data of a target well; 2, value simulation: determining microbial recovery environment adaptability requirements based on a concrete design scheme required by the microbial enhanced oil recovery; 3, determining a sample burying depth through the well ore analysis data; 4, in-situ recovery: recovering required endogenous microbial resources on the scene, and recovering an oil sample or an oil water sample fluid at a specific oil reservoir target burying depth through a PVT (pressure, volume and temperature) instrument according to the value stimulation result; and 5, carrying out subsequent microbial diversity analysis and screening. The method is suitable for the endogenous microbial recovery under various temperature, pressure, pH, mineralization, burial depth oil-reservoir geology conditions, is especially suitable for the deep-well microbial recovery of high-temperature and high-pressure oil reservoirs, and has uses comprising the trace monitoring of oil-reservoir harmful microbes, the endogenous / exogenous microbial diversity analysis before and after the action of the microbial enhanced oil recovery, and the like.

Description

technical field [0001] The invention relates to the application of a downhole sampling device (pressure-temperature-volume tester, PVT) in the analysis, detection, development and utilization of microbial resources related to microbial enhanced oil recovery. Background technique [0002] One of the key points of Microbial Enhanced Oil Recovery (MEOR) is to understand the overall information of the microbial community in the complex environment of oil / gas reservoirs. Oil / gas reservoirs are a unique extreme ecological environment, which contains rich microbial groups; after a long period of genetic evolution and environmental stress, such microorganisms and their metabolites usually have unique physiological and biochemical properties; The research and development of quasi-extremophiles is still in its infancy, but it can be expected to have a wide range of potential applications in the petroleum industry and other industrial production. Microbial enhanced oil recovery refers...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/24
Inventor 谢英周理志刘建勋朱剑飞黎真孟祥娟常泽亮
Owner PETROCHINA CO LTD
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