Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacterium drug-resistant gene detection method, gene chip and kit

A technology for detecting kits and drug-resistant genes, which is applied in the field of biomedicine to achieve significant clinical practical value, fast detection speed, high sensitivity and specificity

Active Publication Date: 2013-03-06
NINGBO NO 2 HOSPITAL +2
View PDF3 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PCR hybridization analysis is the most common method to detect bacterial drug resistance genes. However, screening one or several drug resistance genes among many drug resistance genes requires a lot of repetitive work.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterium drug-resistant gene detection method, gene chip and kit
  • Bacterium drug-resistant gene detection method, gene chip and kit
  • Bacterium drug-resistant gene detection method, gene chip and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Using the kit of the present invention to detect drug resistance genes of common clinical drug-resistant bacteria

[0029] 1. Detection target and design of primers and probes

[0030] The detection targets of this embodiment include: Staphylococcus aureus and Enterococcus drug resistance genes in Gram-positive cocci, and Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa in Gram-negative bacilli Resistant genes of spp. and Acinetobacter baumannii.

[0031] The drug resistance genes have no bacterial species-specific differences, including TEM, SHV, CTX-M, DHA, CIT, IMP, VIM, KPC, OXA-23, qnrB, qnrS, mecA, aac(6')-Ie- aph(2”)-Ia, aac(6')-Ib, GyrA and ParC, among them, the aac(6')-Ib gene is designed with 3 mutation sites, namely T304C, T304A, G535T; GyrA designed 3 mutations The sites are N87, Y87, and L83; ParC designed 4 mutation sites, namely I80, K84, G84, and V84.

[0032] The sequence length of the forward primer and the reve...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bacterium drug-resistant gene detection method, a gene chip and a kit. The kit comprises a primer pair for amplifying the bacterium drug-resistant gene to be detected, and detection probes. The gene chip comprises probes for detecting the bacterium drug-resistant gene to be detected. The method for detecting the drug-resistant gene by using the kit and gene chip comprises the following steps: 1) carrying out PCR (polymerase chain reaction) amplification on the drug-resistant gene in the sample DNA (deoxyribonucleic acid) to be detected by using the primers in the kit; 2) carrying out fluorescence labeling reaction on the amplified product and the detection probes in the kit; and 3) hybridizing the fluorescence-labeled amplified product with the gene chip to determine the drug-resistant gene contained in the sample. The detection method disclosed by the invention covers the detection of the most common 16 drug-resistant genes in clinic, and is beneficial to implementing rapid diagnosis of clinical bacterium drug resistance conditions, thereby providing supports for reasonably selecting therapeutic drugs in clinic.

Description

Technical field [0001] The invention relates to the field of biomedicine, in particular to a method for detecting drug resistance genes of common clinical bacteria. The invention also relates to a kit and a gene chip used in the method. Background technique [0002] Statistics from the National Bacterial Resistance Surveillance Network show that among drug-resistant bacteria, Gram-negative bacteria account for about 70%. Among them, Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Pseudomonas aeruginosa, and Bauman bacteria account for about 70%. There are many kinesin bacteria, and the multi-drug resistance is serious; Gram-positive bacteria account for about 30%, of which Staphylococcus accounts for 2 / 3, while Staphylococcus aureus accounts for almost half of the Staphylococcus genus, and Enterococcus spp. About 1 / 4 of Gram-positive bacteria, and mainly Enterococcus faecalis and Enterococcus faecium. [0003] With the increase of infectious diseases, the third and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12Q1/02
Inventor 蔡挺张春秀张顺陈金丝李巧云周佳菁肖华胜
Owner NINGBO NO 2 HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com