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Quinoline degrading bacteria QG6 with heterotrophic nitrification-aerobic denitrification function and phosphorous removal function and application thereof

A technology of aerobic denitrification and heterotrophic nitrification, which is applied in the direction of aerobic process treatment, bacteria, chemical instruments and methods, etc. It can solve the problems of unstable denitrification and phosphorus removal, high one-time cost, and insufficient effluent quality compliance rate, etc. problems, achieve good economic and environmental benefits, save equipment and investment costs, and shorten system response time

Active Publication Date: 2013-03-20
北京大学科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In traditional biological nitrogen and phosphorus removal, there are contradictions between nitrification, denitrification and phosphorus removal processes for dissolved oxygen, competition between phosphorus accumulating bacteria and denitrifying bacteria on carbon sources, and contradictions between heterotrophic bacteria and autotrophic bacteria on sludge age. These problems will lead to a series of problems in the process of process operation, such as high one-time cost, high energy consumption, large land occupation, unstable denitrification and phosphorus removal effect, and insufficient effluent quality compliance rate.

Method used

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  • Quinoline degrading bacteria QG6 with heterotrophic nitrification-aerobic denitrification function and phosphorous removal function and application thereof
  • Quinoline degrading bacteria QG6 with heterotrophic nitrification-aerobic denitrification function and phosphorous removal function and application thereof
  • Quinoline degrading bacteria QG6 with heterotrophic nitrification-aerobic denitrification function and phosphorous removal function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Implementation Example 1: Pseudomonas sp.QG6 Degradability Determination of Quinoline

[0037] Inoculate Pseudomonas sp.QG6 (preserved on November 9, 2012 in the General Microorganism Center of China Committee for Microbial Culture Collection (CGMCC for short), the preservation number is CGMCC No.6813, the same below) in 100ml of LB In the medium (yeast extract 5g / L, peptone 10g / L, NaCl 10g / L), add 100mg / L quinoline to prevent the invasion of miscellaneous bacteria and maintain the growth vitality of the bacteria, at 30°C, 120rpm, shake the shaker Enrichment culture. After 48 hours of shaking culture, centrifuge at 4000r / min for 10 minutes, collect the bacteria, and use inorganic salt medium (K 2 HPO 4 ·3H 2 O 0.0737g / L, MgSO 4 ·7H 2 O 0.2g / L, FeSO 4 ·7H 2 O 0.02g / L, CaCl 2 2H 2 O 0.002g / L, NaCl 1.0g / L, 0.1% spare trace element solution; trace element stock solution components are: MnSO 4 ·H 2 O 0.2g / L, CuSO 4 ·5H 2 O 0.1g / L, ZnSO4 7H 2 O 0.2g / L, CoCl 2 ·...

Embodiment 2

[0041] Implementation Example 2: Determination of Heterotrophic Nitrification-Aerobic Denitrification Performance of Pseudomonas sp.QG6

[0042] Get 5mL according to the Pseudomonas sp.QG6 bacterium suspension prepared by the method for example 1 and carry out 2 groups of experiments respectively, each group adds three containing 200mL test medium respectively (test medium 1:5.628g / L butanediol Sodium hexahydrate, 0.382g / L NH 4 Cl (C / N=10), 0.79g / L K 2 HPO 4 ·3H 2 O, 0.2g / L MgSO 4 ·7H 2 O, 0.02g / LFeSO 4 ·7H 2 O, 0.002g / L CaCl 2 2H 2 O, 1.0g / LNaCl, 0.1% standby trace element solution in Example 1:; test medium 2: 11.256g / L sodium succinate hexahydrate, 0.764g / LNH 4 Cl (C / N=10), 0.79g / L K 2 HPO 4 ·3H 2 O, 0.2g / L MgSO 4 ·7H 2 O, 0.02g / L FeSO 4 ·7H 2 O, 0.002g / L CaCl 2 2H 2 O, 1.0g / L NaCl, 0.1% of the spare trace element solution in Example 1; pH 7.5~8.0), sealed with 9 layers of gauze, and cultured in a shaker at 30°C and 150rpm. The test medium that was not in...

Embodiment 3

[0046] Implementation Example 3: Determination of the Synchronous Denitrification and Phosphorus Ability of Pseudomonas sp.QG6

[0047] Get 5mL according to the Pseudomonas sp.QG6 bacterium suspension prepared by the method for example 1 and carry out 2 groups of experiments respectively, each group adds three containing 200mL test medium respectively (test medium 1:5.628g / L butanediol Sodium hexahydrate, 0.382g / L NH 4 Cl, 0.0737g / L K 2 HPO 4 ·3H 2 O, 0.2g / L MgSO 4 ·7H 2 O, 0.02g / LFeSO 4 ·7H 2 O, 0.002g / L CaCl 2 2H 2 O, 1.0g / L NaCl, 0.1% spare trace element solution in Example 1; test medium 2: 5.628g / L sodium succinate hexahydrate, 0.382g / LNH 4 Cl, 0.1474g / L K 2 HPO 4 ·3H 2 O, 0.2g / LMgSO 4 ·7H 2 O, 0.02g / L FeSO 4 ·7H 2 O, 0.002g / L CaCl 2 2H 2 O, 1.0g / L NaCl, 0.1% of the spare trace element solution in Example 1; pH 7.5~8.0), sealed with 9 layers of gauze, and cultured in a shaker at 30°C and 150rpm. The test medium that was not inoculated with the bacterial...

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Abstract

The invention relates to a quinoline degrading bacteria QG6 with a heterotrophic nitrification-aerobic denitrification function and a phosphorous removal function, and application thereof. The quinoline degrading bacteria provided by the invention belongs to pseudomonas sp. and is named as QG6. The bacterial strain can adopt quinoline as the only carbon source, nitrogen source and energy to carry out growth and reproduction, so that 100 mg / L and 150mg / L of quinoline can be completely degraded within 12 hours; organic carbon can be further utilized as the only carbon source, and ammonia nitrogen is utilized as the only nitrogen source, so that the ammonia nitrogen is directly converted to a gas product through the heterotrophic nitrification-aerobic denitrification function for completing denitrification; and inorganic phosphor is taken in and converted to components of the body for removing the phosphor under an aerobic condition. The bacterial strain can efficiently degrade quinoline, and can synchronously remove nitrogen and phosphor when quinoline is degraded in the presence of other usable carbon sources under a single aerobic condition, so degradation-resistant organic substances in the sewage can be removed, and a biological nitrogen and phosphorus removal purpose can be achieved.

Description

technical field [0001] The invention relates to a quinoline-degrading bacterium QG6 with the functions of heterotrophic nitrification-aerobic denitrification and phosphorus removal and its application. The quinoline-degrading bacterium QG6 belongs to Pseudomonas sp., has the function of degrading quinoline, and also has the function of heterotrophic nitrification-aerobic denitrification, and can be used in the process of simultaneous nitrification and denitrification denitrification Complete the removal of phosphorus-containing pollutants in sewage, so as to achieve the purpose of removal of refractory organic matter in sewage and simultaneous biological nitrogen and phosphorus removal. Background technique [0002] Quinoline is a typical polycyclic aromatic nitrogen-containing heterocyclic compound. It has the characteristics of high toxicity, strong teratogenicity and carcinogenicity, and is difficult to biodegrade. It will pollute groundwater resources through soil and ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/38C02F101/38C02F101/10
CPCY02W10/10
Inventor 倪晋仁段明君
Owner 北京大学科技开发有限公司