Flow cytometry device for no-flow type cytometry box and flow cytometry method thereof

Abstract

The invention discloses a flow cytometry device for a no-flow type cytometry box and a flow cytometry method for the no-flow type cytometry box. The device adopts a unique kurt microporous direct coaxial illumination method, so that the necessary expensive flow cytometry box and the complex liquid focusing in the existing mainstream flow cytometry can be thoroughly abandoned, the structure can be simplified, the cost can be reduced, the interaction time between the cell and the excited laser can be prolonged by tens of times, the sensitivity of the apparatus can be improved by orders of magnitude, the backward scattering light which is most sensitive to the structures and the components in the cells can be firstly and successfully applied to the actual commercial flow cytometry due to the unique structure, and the identifying capability of the apparatus to the different cell subsets can be improved. A multi-wavelength excited laser combiner and a multi-wavelength fluorescent detection system of a new device all adopt a single dispersing element, so that the loss of the apparatus can be reduced to the lowest level, and the overlap of the wavelengths can be reduced. Compared with the flow type cytometry which is universally used in the world, the flow cytometry device is obvious in cost performance and market advantage.

Description

Technical field: [0001] The invention relates to a flow cytometer device and method of a medical detection instrument, in particular to a flow cytometer device and method without a flow cell box. Background technique: [0002] 1. Flow Cytometry (FCM) and Flow Cytometry [0003] Flow cytometry (FCM) is developed under the combination of microscope technology, staining chemistry, electronics technology and computer and other fields to characterize the characteristics and components of single cells in fast linear flow, or other A technology for multi-parameter analysis and sorting of various tiny particles (such as bacteria) and their loads. It can not only measure cell size and internal particle properties, but also detect cell surface and cytoplasmic antigens, and intracellular DNA and RNA content. etc., the population cells can be analyzed at the single-cell level. Detect and analyze a large number of cells in a short period of time, collect, store and process data, and pe...

AI Technical Summary

Problems solved by technology

[0021] 7. Backward light scattering

It can be noticed that the fluorescent signals of different wavelengths are mixed in the same light path when they come out from the microscope objective lens (5-4) for collecting fluorescence and side light scattering. Wherever the fluorescent signals of a single wavelength are to be separated, it is necessary to use at least A beam splitter, a filter (sometimes multiple), there are 10 such lenses in Figure 5, not only the structure is complex, the optical path is difficult to adjust, but also the loss is large

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