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Application of doxycycline in preparing medicament for treating epithelial ovarian cancer

A doxycycline and ovarian cancer technology, applied in the field of medicine, can solve the problems of no doxycycline epithelial ovarian cancer, etc., and achieve the effect of small side effects and convenient administration

Inactive Publication Date: 2013-04-03
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report on the use of doxycycline in the treatment of epithelial ovarian cancer

Method used

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  • Application of doxycycline in preparing medicament for treating epithelial ovarian cancer
  • Application of doxycycline in preparing medicament for treating epithelial ovarian cancer
  • Application of doxycycline in preparing medicament for treating epithelial ovarian cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1, doxycycline to ovarian cancer cell proliferation inhibitory experiment

[0012] Doxycycline, a product of Sigma Company, has a molecular formula of (C22H24N2O8·HCl)2·C2H6O·H2O, and a molecular weight of 1025.89. (the same below)

[0013] Ovarian cancer cells SKOV3 and its drug-resistant strain SKOV3 / DDP were purchased from ATCC (American Type Culture Collection). (the same below)

[0014] To prepare doxycycline solution:

[0015] The doxycycline powder was prepared with sterile water for injection into a solution with an initial concentration of 5 mg / ml, and stored in a -20°C refrigerator for future use.

[0016] Prepare cell suspension:

[0017] SKOV 3 and ovarian cancer cell drug-resistant strain SKOV 3 / DDP were prepared with 1640 medium containing 10% fetal bovine serum to obtain a cell concentration of 5×10 4 cells / ml cell suspension for later use.

[0018] The experiment was divided into two groups, A and B, and group A was ovarian cancer cel...

Embodiment 2

[0030] Example 2, Doxycycline's Inhibitory Effect Experiment on Ovarian Cancer Cell Invasion Ability

[0031] This experiment uses the Transwell chamber (purchased from BD Biotechnology Co., Ltd.), which is different from ordinary culture plates. It is divided into an upper chamber and a lower chamber. The lower chamber is equivalent to each well on a 24-well culture plate, but the aperture is relatively large. The upper chamber is a small chamber placed in the lower chamber. Each hole in the lower chamber is equipped with a small chamber. This small chamber is also called the upper chamber. The bottom of the small chamber is a layer of fiber polyester film with small holes of 6-8 μm in diameter. It is called the basement membrane. During cell culture, the cells attached to the basement membrane at the bottom of the upper chamber can pass through the small hole and enter the lower chamber from the upper surface of the basement membrane to adhere to the lower surface of the base...

Embodiment 3

[0047] Example 3, Doxycycline's promoting effect on ovarian cancer cell apoptosis

[0048] The experiment was divided into two groups, A and B, and group A was ovarian cancer cell SKOV 3 , group B is ovarian cancer drug-resistant cell line SKOV 3 / DDP. There were 5 groups in each group, which were two blank control groups and three doxycycline groups. The drug concentrations were 5, 10, and 20 μg / ml respectively. There was no duplicate hole, and the experiment was repeated 3 times.

[0049] 1. The above-mentioned initial concentration of 5 mg / ml doxycycline solution was formulated into 5, 10, and 20 μg / ml doxycycline solutions with 1640 medium containing 10% fetal bovine serum respectively;

[0050] 2. The ovarian cancer cells SKOV 3 SKOV 3 / DDP was cultured with 1640 containing 10% fetal bovine serum as usual and cultured in a culture flask at 37°C for 36 hours. At this time, the cell growth was in the logarithmic phase. 6 cells / ml cell suspension, group A and group B we...

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Abstract

The invention relates to the technical field of medicines, and in particular relates to a novel application of doxycycline in preparing a medicament for treating epithelial ovarian cancer. Animal experiments and in-vitro cell experiments prove that doxycycline can inhibit multiplication and invasion of ovarian cancer cells, can prevent epithelial ovarian cancer cells from transferring, can promote apoptosis of epithelial ovarian cancer cells, has a remarkable inhibition effect to ovarian cancer cells, in particular platinum-based drug-resistance ovarian cancer cells, and can inhibit growth of ovarian cancer tissues and formation of ascites, so that doxycycline can be used for preparing a medicament for treating epithelial ovarian cancer. The invention provides a novel application to doxycycline, and compared with other chemotherapeutic drugs, doxycycline has the advantages of convenient administration and small side effect in the field of treating epithelial ovarian cancer.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to a new application of doxycycline in the preparation of drugs for treating epithelial ovarian cancer. Background technique [0002] Doxycycline is a tetracycline family broad-spectrum antibiotic, usually used in the treatment of infectious diseases, its antibacterial spectrum in addition to common pathogenic bacteria, Rickettsia, Mycoplasma, Chlamydia, atypical branch bacilli were sensitive to doxycycline. Doxycycline has strong antibacterial effect on pneumococcus, good antibacterial effect on Haemophilus Duke, Brucella, Vibrio cholerae and Yersinia, 70% of anaerobic bacteria Cyclocycline sensitive. In recent years, studies have found that doxycycline can inhibit the proliferation of colorectal cancer and non-small cell lung cancer cells, and can also induce apoptosis of leukemia cell lines, osteosarcoma cells, melanoma cells, and breast cancer cells. But so far there is no rep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/65A61P35/00
Inventor 徐明娟吴薇马蓓管睿蔡圣芸王丽娅
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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