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Method for preparing high-purity heme on large scale and application of heme

A large-scale preparation of heme technology, applied in the biological field, can solve the problems of health hazards for production personnel, difficulty in large-scale production, and large-scale environmental pollution

Inactive Publication Date: 2013-04-10
SHANGHAI GENON BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for producing heme iron mainly include: acetone method, glacial acetic acid method, surfactant method, etc. The main disadvantage is that toxic chemical reagents are used in the production process, resulting in relatively large environmental pollution, and large-scale production is difficult.
Among them, the preparation of heme by the acetone method is not only harmful to the health of production personnel, but also there is acetone residue in the product

Method used

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  • Method for preparing high-purity heme on large scale and application of heme
  • Method for preparing high-purity heme on large scale and application of heme
  • Method for preparing high-purity heme on large scale and application of heme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Production of high-purity heme iron from pig blood

[0106] (1) Mix 3 tons of blood cells from pigs with 12 tons of pure water and stir for 8 hours to obtain hemoglobin liquid (ie hemoglobin liquid I) with membrane rupture; the blood cells are fresh and healthy blood cells; the water is pure water;

[0107] (2) 15 tons of hemoglobin liquid I of membrane rupture is adopted tubular centrifugation to remove cell membrane; the centrifugal speed is 12000 rpm, and the discharge from the slag outlet at the lower end is cell membrane;

[0108] (3) Mix 20 kg of hydrochloric acid (concentration 38% (w / v)) with 63 kg of pure water (the final concentration of hydrochloric acid is about 9% (w / v)), and slowly add it to the 15 tons of hemoglobin solution that has been removed from the membrane. Stir for 3 hours, heme and globin are separated, and a mixture of heme and globin is obtained; the pH is about 3;

[0109] (4) Slowly add 3 tons of 1% CMC-NA into 15 tons of the mixed solution...

Embodiment 2

[0120] Production of heme iron from poultry blood

[0121] (1) Mix 3 tons of blood cells from poultry with 12 tons of pure water, stir for 8 hours to obtain hemoglobin liquid with ruptured membranes, and obtain hemoglobin liquid I; the blood cells are fresh and healthy blood cells; the water is pure water;

[0122] (2) The 15 tons of hemoglobin liquid with ruptured membranes was centrifuged by tube to remove cell membranes; the centrifugal speed was 12000 rpm, and the discharge from the slag outlet at the lower end was cell membranes;

[0123] (3) Mix 20 kg of hydrochloric acid (concentration 38% (w / v)) with 63 kg of pure water (the final concentration of hydrochloric acid is about 9% (w / v)), slowly add 15 tons of hemoglobin solution without membrane, and stir After 3 hours, a mixture of heme and globin was obtained; the pH was about 3;

[0124] (4) Slowly add 3 tons of 1% CMC-NA to 15 tons of the mixed solution, stir for 3 hours, and let stand for 10 hours to obtain the mixe...

Embodiment 3

[0135] Viscosity adjustment experiment

[0136] (1) Mix 3 tons of blood cells from poultry with 6 tons of pure water, stir for 8 hours to obtain hemoglobin liquid with ruptured membranes, and obtain hemoglobin liquid I; the blood cells are fresh and healthy blood cells; the water is pure water;

[0137] (2) Centrifuge the 9 tons of hemoglobin solution with membrane rupture to remove the cell membrane; the centrifugal speed is 12000 rpm, and the discharge from the slag outlet at the lower end is the cell membrane;

[0138] (3) Mix 20 kg of hydrochloric acid (concentration 38% (w / v)) with 63 kg of pure water (the final concentration of hydrochloric acid is about 9% (w / v)), slowly add 9 tons of hemoglobin solution without membrane, and stir After 3 hours, a mixture of heme and globin was obtained; the pH was about 3;

[0139] (4) Slowly add 3 tons of 5% CMC-NA to 9 tons of the mixed solution, stir for 3 hours, and let stand for 10 hours to obtain the mixed solution II of hemoglo...

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Abstract

The invention relates to a method for preparing high-purity heme on a large scale and an application of the heme. The invention provides the method for preparing the high-purity heme on a large scale for the first time. The method comprises the steps of solid-liquid separation, adsorption, centrifugation, repeated enzymolysis and the like, and has the advantages that the purity of heme is improved. Particularly, under the condition of a certain pH value (the pH value equals 1 to 4), adsorption reaction between the separated heme and CMC-NA (sodium carboxymethyl cellulose) or carboxymethyl starch is performed efficiently; through repeated special enzymolysis and washing, the prepared heme has high iron content, good palatability, and high adsorption and utilization rate, is environment-friendly and pollution-free during production, has no toxicity or adverse effects, and is very suitable for feeds and food for supplementing iron.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a large-scale preparation method and application of high-purity hemoglobin. Background technique [0002] Recently, the scale of the slaughtering industry in the world is expanding day by day. When slaughtering livestock and poultry to produce meat, a large amount of blood and other by-products are produced. If these huge by-product resources cannot be used or disposed of in a reasonable and timely manner, it will not only cause environmental pollution, but also cause resource pollution. serious waste. [0003] In my country, the annual production of livestock and poultry by-product blood resources exceeds 3.3 million tons. Animal blood contains almost all the nutrients and elements required by the human body and other animals, including nutrients such as protein, amino acids, vitamins, and trace elements such as sodium, potassium, iron, and calcium. At prese...

Claims

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Application Information

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IPC IPC(8): C07D487/22A23L1/302A23L1/30A23K1/04A23K1/16A61P3/02A23K10/24A23L33/15
Inventor 成国祥江国永于伟
Owner SHANGHAI GENON BIOLOGICAL PROD
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