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Method for preparing rice protein active polypeptide through continuous enzymolysis and secondary membrance filtering and purification

A technology of rice protein and active peptides, which is applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of high energy consumption, heavy product bitterness, low utilization rate of protease, etc., and achieve the goal of reducing cholesterol and low cost Effect

Active Publication Date: 2013-04-17
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing preparation method of rice protein active polypeptide is intermittent enzymatic hydrolysis, followed by vacuum concentration and drying preparation process. The intermittent enzymatic hydrolysis method has the following disadvantages: the utilization rate of protease is low, excessive enzymolysis leads to heavy bitterness of the product, and the extraction rate of active polypeptide is low. , high energy consumption, dark color, and cannot be continuously produced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The method for preparing rice protein active polypeptide by continuous enzymolysis and secondary membrane filtration purification, the steps are as follows:

[0016] (1) Dissolve alkaline protease in sodium alginate solution with a mass concentration of 4%, disperse into small droplets and fall into CaCl with a mass concentration of 1.5% 2 Spherical immobilized enzymes are formed in the solution, solidified for 30 minutes, filtered through a 40-mesh filter to recover the immobilized enzymes, the enzyme activity is 15000IU / g, and loaded into the enzymolysis reactor. Alkaline protease activity was determined by the Folin-phenol method.

[0017] (2) Prepare a rice protein solution with a mass concentration of 4%, adjust the pH value to 9.0 with 2mol / L NaOH solution, heat to a constant temperature of 55°C, and then pour it into the enzymolysis reactor.

[0018] (3) The enzymolysis reactor is connected to the ultrafiltration membrane system with a molecular weight cut-off o...

Embodiment 2

[0021] The method for preparing rice protein active polypeptide by continuous enzymolysis and secondary membrane filtration purification, the steps are as follows:

[0022] (1) Dissolve alkaline protease in sodium alginate solution with a mass concentration of 1.0%, disperse into small droplets and fall into CaCl with a mass concentration of 1.0% 2 Spherical immobilized enzyme is formed in the solution, solidified for 30 minutes, filtered through a 40-mesh filter to recover the immobilized enzyme, the enzyme activity is 5000IU / g, and loaded into the enzymolysis reactor. Alkaline protease activity was determined by the Folin-phenol method.

[0023] (2) Prepare a rice protein solution with a mass concentration of 8%, adjust the pH value to 9.0 with 2mol / L NaOH solution, heat to a constant temperature of 55°C, and then pour it into the enzymolysis reactor.

[0024] (3) The enzymolysis reactor is connected to the ultrafiltration membrane system with a molecular weight cut-off of ...

Embodiment 3

[0027] The method for preparing rice protein active polypeptide by continuous enzymolysis and secondary membrane filtration purification, the steps are as follows:

[0028] (1) Dissolve alkaline protease in a sodium alginate solution with a mass concentration of 2.5%, disperse into small droplets and fall into a CaCl solution with a mass concentration of 3.5% by rotating and centrifuging a porous centrifugal disc 2 Spherical immobilized enzymes are formed in the solution, solidified for 30 minutes, filtered through a 40-mesh filter to recover the immobilized enzymes, the enzyme activity is 9000IU / g, and loaded into the enzymolysis reactor. Alkaline protease activity was determined by the Folin-phenol method.

[0029] (2) Prepare a rice protein solution with a mass concentration of 6%, adjust the pH value to 9.0 with 2mol / L NaOH solution, heat to a constant temperature of 55°C, and then pour it into the enzymolysis reactor.

[0030] (3) The enzymolysis reactor is connected to ...

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PUM

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Abstract

The invention discloses a method for preparing rice protein active polypeptide through continuous enzymolysis and secondary membrane filtering and purification. The method comprises the steps that alkaline protease and rice protein are taken as principal raw materials for enzymolysis reaction; the raw materials are subjected to ultrafiltration membrane filtering, nanofiltration membrane filtering and spray drying; and thenrice protein active polypeptide is obtained. Compared with theprior art, the method has the benefits that the method can avoid product bitterness due to excess enzymolysis; proteolytic enzyme can be recycled after immobilized; the utilization ratio of enzyme is increased; and continuous production can be achieved. Active polypeptide prepared by the method is high in purity, safe and harmless, wide in raw material source, and low in manufacturing cost, and has very high popularization values in the fields of food, cosmetic, health care and medicine.

Description

technical field [0001] The invention belongs to the technical field of deep processing of grain by-products, in particular to a method for preparing rice protein active polypeptide through continuous enzymatic hydrolysis and secondary membrane filtration purification. Background technique [0002] Free radicals produced by human metabolism have a strong killing effect on body tissue cells, which can cause cell death and tissue damage, such as atherosclerosis, cancer, lung injury and aging. Antioxidant peptides, as a non-enzymatic antioxidant, have become a research hotspot in recent years because of their high biological activity and safety. [0003] my country's rice production ranks first in the world, and the rice protein produced in the deep processing of rice has high biological value. Rice protein is a high-quality plant protein, which has the advantages of reasonable balance of amino acid composition, mild taste, hypoallergenicity and lower cholesterol; moreover, ric...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C12N11/10
Inventor 夏宁滕建文劳泰财
Owner GUANGXI UNIV
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