Method for preparing rice protein active polypeptide through continuous enzymolysis and secondary membrance filtering and purification

Abstract

The invention discloses a method for preparing rice protein active polypeptide through continuous enzymolysis and secondary membrane filtering and purification. The method comprises the steps that alkaline protease and rice protein are taken as principal raw materials for enzymolysis reaction; the raw materials are subjected to ultrafiltration membrane filtering, nanofiltration membrane filtering and spray drying; and thenrice protein active polypeptide is obtained. Compared with theprior art, the method has the benefits that the method can avoid product bitterness due to excess enzymolysis; proteolytic enzyme can be recycled after immobilized; the utilization ratio of enzyme is increased; and continuous production can be achieved. Active polypeptide prepared by the method is high in purity, safe and harmless, wide in raw material source, and low in manufacturing cost, and has very high popularization values in the fields of food, cosmetic, health care and medicine.

Description

technical field

[0001] The invention belongs to the technical field of deep processing of grain by-products, in particular to a method for preparing rice protein active polypeptide through continuous enzymatic hydrolysis and secondary membrane filtration purification. Background technique

[0002] Free radicals produced by human metabolism have a strong killing effect on body tissue cells, which can cause cell death and tissue damage, such as atherosclerosis, cancer, lung injury and aging. Antioxidant peptides, as a non-enzymatic antioxidant, have become a research hotspot in recent years because of their high biological activity and safety.

[0003] my country's rice production ranks first in the world, and the rice protein produced in the deep processing of rice has high biological value. Rice protein is a high-quality plant protein, which has the advantages of reasonable balance of amino acid composition, mild taste, hypoallergenicity and lower cholesterol; moreover, ric...