Process for extracting mannan from beer yeast powder

A technology of brewer's yeast powder and mannan, which is applied in the field of bioengineering, can solve the problems of low product purity and environmental pollution, and achieves the effects of high physical and chemical stability, reduced production cost and good enrichment effect.

Active Publication Date: 2013-04-24
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a process for extracting mannan from beer yeast powder, which solves the problem of the need to use a large amount of organic solvents and acid-base solutions in the process of extracting mannan, which causes environmental pollution and product purity. not high problem

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  • Process for extracting mannan from beer yeast powder
  • Process for extracting mannan from beer yeast powder
  • Process for extracting mannan from beer yeast powder

Examples

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Effect test

Embodiment 1

[0045] A process for extracting mannan in brewer's yeast powder, using brewer's yeast powder as a substrate, comprising the steps of:

[0046] (1) Enzymolysis and alkali extraction: Weigh 48Kg of the substrate brewer’s yeast powder, add 352Kg of water, stir and mix well, adjust the pH value to 6 with glacial acetic acid solution with a mass fraction of 5%, add yeast to extract at a temperature of 60°C Enzyme hydrolysis with 2Kg of enzyme and 2Kg of neutral protease for 2 hours to obtain an enzymolysis solution; then add 8.08 Kg of sodium hydroxide solid to the enzymolysis solution and stir at 65°C for 1 hour to obtain an extract;

[0047] (2) Microfiltration: The extract obtained in step (1) is separated into a heavy phase and a light phase by a centrifuge, and the heavy phase is then filtered by a filter press to separate the slag and the filtrate, and the filtrate is mixed with the light phase Finally, the ceramic membrane microfiltration equipment with a pore size of 0.2 μm...

Embodiment 2

[0053] A process for extracting mannan in brewer's yeast powder, using brewer's yeast powder as a substrate, comprising the steps of:

[0054] (1) Enzymolysis and alkali extraction: Weigh 60Kg of the substrate brewer’s yeast powder, add 340Kg of water, stir and mix well, adjust the pH value to 5.5 with glacial acetic acid solution with a mass fraction of 20%, and add yeast to extract at a temperature of 55°C 0.4Kg of enzyme and 0.4Kg of alkaline protease were used for enzymolysis for 8 hours to obtain an enzymolysis solution; then 24.05 Kg of potassium hydroxide solid was added to the enzymolysis solution, and stirred at 60°C for 2 hours to obtain an extract;

[0055] (2) Microfiltration: The extract obtained in step (1) is separated into a heavy phase and a light phase by a centrifuge, and the heavy phase is then filtered by a filter press to separate the slag and the filtrate, and the filtrate is mixed with the light phase Finally, the ceramic membrane microfiltration equipm...

Embodiment 3

[0061] A process for extracting mannan in brewer's yeast powder, using brewer's yeast powder as a substrate, comprising the steps of:

[0062] (1) Enzyme hydrolysis and alkali extraction: Weigh 140Kg of the substrate beer yeast powder, add 860Kg of water, stir and mix well, adjust the pH value to 7.5 with glacial acetic acid solution with a mass fraction of 10%, and add yeast to extract at a temperature of 57°C 2Kg of enzyme and 4Kg of alkaline protease were subjected to enzymolysis for 6 hours to obtain an enzymolysis solution; then 100.6 Kg of sodium hydroxide solid was added to the enzymolysis solution, and stirred at 75°C for 0.5 hours to obtain an extract;

[0063] (2) Microfiltration: The extract obtained in step (1) is separated into a heavy phase and a light phase by a centrifuge, and the heavy phase is then filtered by a filter press to separate the slag and the filtrate, and the filtrate is mixed with the light phase Finally, the ceramic membrane microfiltration equi...

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Abstract

The invention relates to a process for extracting mannan from beer yeast powder. The beer yeast powder is used as a substrate, and the process comprises the steps of enzymolysis extraction, alkaline extraction, microfiltration and ultrafiltration of an extracting solution, recovery of alkali liquor through nanofiltration, purification by using macroporous adsorption resin, spray drying and the like. The process combines an enzyme method with an alkaline method, an industrial alkali-resistant membrane separation technology and a macroporous adsorption resin purification technology are used, an organic solvent is not used, an alkali-resistant nanofiltration membrane system can recover 90 percent of extracting alkali liquor, and the extracting alkali liquor is reused in the extraction steps, so that the pollution to the environment is greatly reduced, the produced mannan is high in yield and purity, low in production cost, and suitable for large-scale production.

Description

Technical field [0001] The present invention is the field of biological engineering technology. It involves a separate and purified process of biological activated polysaccharides, which specifically involves the extraction process of glycotium glycogen in a beer yeast powder. Background technique [0002] The glycosaccharides exist on the outer layer of the yeast cell wall, accounting for about 40 % of the dry weight of the cell wall, giving cell biological activity and controlling cell wall pores.Glitanose has an immune regulation function, and has a unique effect on the prevention and treatment of tumor and cardiovascular disease. It has physiological activity such as antiviral, bacterial and fungal infections, antitumor, antioxidant and anti -radiation, and promoting wound healing. [0003] Generally, the extraction of polysaccharides is extracted with alkaline solution. After the extraction is extracted, the metering is neutralized. After the solid solid is separated, the ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 周传兵徐泽平刘结磊冯文娟高洪奎
Owner CHAMBROAD CHEM IND RES INST CO LTD
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