Naringinase fermentation medium

Abstract

The invention relates to a naringinase fermentation medium. The naringinase fermentation medium comprises the following components in parts by weight: 100 parts of pomelo peel powder, 4-20 parts of ammonium salt (or urea) and 100-300 parts of water. In the naringinase fermentation medium, the pomelo peel is used as the principal raw material for the naringinase fermentation and ammonium salt or urea is used as nitrogen source, the repression function of glucose, fructose, starch and the like to catabolism of naringinase fermentation is eliminated. As the industrial processing of pomelo has been achieved, the pomelo peel as the side product is stable in resource; in addition, the technology of peeling firstly and then squeezing juice is used in the pemelo processing, thus the rest peel, compared with the processing side product of orange, tangerine and grapefruit (pomelo), has the advantages of high naringin and aurantiamarin content, low glucose content and low fructose content. Therefore, the fermentation medium provided by the invention has the characteristics of stable resource, low cost, high naringinase fermentation yield, and the like, and is beneficial for industrial production. The fermentation medium can produce naringinase in high yield whatever uncooked material fermentation and cooked material fermentation is used.

Description

Technical field [0001] The invention relates to a microbial fermentation culture medium, in particular to a culture medium for naringinase industrial fermentation and an application method thereof. Background technique [0002] Naringinase (Naringinase) is a glycosidase that hydrolyzes naringin (chemical name 4, 5, 7-trihydroxyflavanone-7-rhamnosyl glucoside) to naringenin. It It has the activity of α-L-rhamnosidase (EC3.2.1.40) and β-D-glucosidase (EC3.2.1.21). [0003] People have conducted long-term research on the liquid and solid fermentation production technology of naringinase. In 2011, Puri and others used Staphylococcus xylosus The MAK2 strain fermented orange peel to produce naringinase. Studies have shown that adding orange peel can significantly increase the enzyme production, and the enzyme production can reach 8.9 IU / mL. In the same year, Mutuswamy et al. fermented naringinase with rice bran, wheat bran, bagasse, orange peel, etc. as solid raw materials, and found...

AI Technical Summary

Problems solved by technology

The medium raw materials for solid-state fermentation of naringinase reported at present have the following deficiencies: (1) Orange peels, orange peels and grapefruit (grapefruit) peels left in fresh food are not applicable due to collection difficulties and unstable sources It is used for industrial fermentation of naringinase; (2) bagasse and other carbon sources need to add naringin and hesperidin to synthesize naringinase due to the lack of inducers, and the cost is high; (3) peptone, yeast extract and beef extract Nitrogen sources such as nitrogen sources are only suitable for laboratory research due to their high price, and their use in industrial fermentation will greatly increase the cost; (4) bean cake powder, bran, etc. are prone to catabolism due to the large amount of starch contained, and the synthesis of naringinase Restricted, difficult to achieve high-yield fermentation

Therefore, the raw materials of the naringinase fermentation medium reported at present cannot be applied to industrial production due to reasons such as unstable sources, high prices, and catabolism. The key to industrial fermentation of sidase

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