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Antibody against heavy metal cadmium and application of same in detection of cadmium residue in traditional Chinese medicine

A technology of heavy metals and antibodies, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of tedious and time-consuming sampling, transportation and storage, and limited selection of complexing agents, and achieve good sensitivity, good application prospects, and stable antibodies Effect

Active Publication Date: 2013-05-01
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is still limited due to the limited choice of complexing agents
[0004] The above cadmium ion detection methods mostly use expensive chemical instruments, which require skilled operators and are carried out in a laboratory far away from the sampling site, and require cumbersome and time-consuming steps such as sampling, transportation and storage.

Method used

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  • Antibody against heavy metal cadmium and application of same in detection of cadmium residue in traditional Chinese medicine
  • Antibody against heavy metal cadmium and application of same in detection of cadmium residue in traditional Chinese medicine

Examples

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Embodiment 1

[0051] 1. Antigen design and synthesis

[0052] Using bifunctional chelating agent isothiocyanate benzylethylenediaminetetraacetic acid (ITCBE) to couple heavy metal cadmium ions to keyhole limpet hemocyanin (KLH), the specific method is:

[0053] Weigh 1.4 mg ITCBE and dissolve it in 1 mL DMSO, slowly add 710 μL of 1000 mg / L cadmium solution standard into the ITCBE solution dropwise, adjust the pH to 7.4 with triethylamine, and stir for 24 hours at 25 °C with a magnetic stirrer. 10mg KLH was dissolved in 20mmol / L Hepes buffer solution, and the above-mentioned chelated reaction solution was slowly added dropwise to the KLH solution, and the pH was adjusted to 9.6 with triethylamine. After stirring with a magnetic stirrer for 24 h at 25° C., it was dialyzed with 5 mmol / L HEPES buffer solution for three days.

[0054] 2. Preparation of Coating Antigen

[0055] Using bifunctional chelating agent isothiocyanate benzylethylenediaminetetraacetic acid (ITCBE), the heavy metal cadmi...

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Abstract

The invention provides an antibody against heavy metal cadmium and application of the same in detection of cadmium residue in a traditional Chinese medicine, which relates to a semi-antigen and an artificial antigen for and an antibody against a heavy metal cadmium ion and preparation methods and application thereof. According to the invention, the problems of tedious and complex procedures, high cost and a slow analysis speed of a traditional physical-chemical analysis method are overcome, and a simple, rapid, sensitive and accurate Immunoassay technology is provided; a complex of isothiocyanobenzyl ethylenediaminetetraacetic acid (ITCBE) and an insulator is used as the semi-antigen and is respectively connected with carrier proteins like haemocyanin (KLH) and bovine serum albumin to synthesize the artificial antigen and an envelope antigen; and the artificial antigen undergoes animal immunization, blood drawing, separation of antiserum and purification to prepare the antibody. The antibody is stable and has good specificity and sensitivity; a synthetic method for the antibody is easy and convenient; and the antibody can be used for rapid immunodetection of the heavy metal cadmium ion and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and residual analysis of heavy metal cadmium ions; relates to inorganic chelation chemistry, immunochemistry, biochemistry and physicochemical testing technology, etc.; particularly relates to a bifunctional chelating agent benzyl ethylenediamine tetraacetic acid isothiocyanate ( ITCBE) design and synthesis of artificial specific antigens, preparation of specific antibodies for immunized animals and their application in the establishment of immunoassay methods. Background technique [0002] Heavy metals are a class of very dangerous pollutants that accumulate in organisms for a long time and cannot be degraded, and even in extremely small amounts can cause adverse consequences. Various ecosystems are affected by heavy metals to varying degrees. Heavy metals are deposited into the human body through the food chain and can cause various diseases. Therefore, the monitoring of heavy metal res...

Claims

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Application Information

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IPC IPC(8): G01N33/531
Inventor 王硕王俊平生威王津
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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