Antibody against heavy metal cadmium and application of same in detection of cadmium residue in traditional Chinese medicine
A technology of heavy metals and antibodies, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of tedious and time-consuming sampling, transportation and storage, and limited selection of complexing agents, and achieve good sensitivity, good application prospects, and stable antibodies Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0051] 1. Antigen design and synthesis
[0052] Using bifunctional chelating agent isothiocyanate benzylethylenediaminetetraacetic acid (ITCBE) to couple heavy metal cadmium ions to keyhole limpet hemocyanin (KLH), the specific method is:
[0053] Weigh 1.4 mg ITCBE and dissolve it in 1 mL DMSO, slowly add 710 μL of 1000 mg / L cadmium solution standard into the ITCBE solution dropwise, adjust the pH to 7.4 with triethylamine, and stir for 24 hours at 25 °C with a magnetic stirrer. 10mg KLH was dissolved in 20mmol / L Hepes buffer solution, and the above-mentioned chelated reaction solution was slowly added dropwise to the KLH solution, and the pH was adjusted to 9.6 with triethylamine. After stirring with a magnetic stirrer for 24 h at 25° C., it was dialyzed with 5 mmol / L HEPES buffer solution for three days.
[0054] 2. Preparation of Coating Antigen
[0055] Using bifunctional chelating agent isothiocyanate benzylethylenediaminetetraacetic acid (ITCBE), the heavy metal cadmi...
PUM

Abstract
Description
Claims
Application Information

- Generate Ideas
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com