Method for detecting quinolone antibiotic by means of electrochemical luminescence
A quinolone, luminescence detection technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve problems such as low detection limit, and achieve the effect of low detection limit, wide linear range and high sensitivity
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Embodiment 1
[0049] Use the MPI-B type electrochemiluminescence analyzer (electrochemical workstation), set the high voltage of the photomultiplier tube to 900V, the photomultiplier tube adopts two-stage amplification, the scanning voltage is 0-2.0V, the working electrode is a GC electrode, and the buffer system is 0.1mol / L phosphate buffer solution with pH=6.5. Select the concentration of n=8 dinuclear bipyridyl ruthenium metal complexes as 1×10 -4 mol / L, add ofloxacin to observe its influence on ECL, detect the luminescent signal of the solution, the results are as follows figure 1 shown. The ECL intensity after adding ofloxacin will be significantly higher than the ECL intensity before adding ofloxacin, and the concentration range of ofloxacin is 1×10 -13 ~1×10 -6 mol / L, the change of ECL luminous intensity (ΔECL) has a good linear relationship with the logarithm of the concentration of ofloxacin. The detection limit of ofloxacin is 1×10 -13 mol / L, the linear correlation coefficien...
Embodiment 2
[0051] Use the MPI-B type electrochemiluminescence analyzer (electrochemical workstation), set the high voltage of the photomultiplier tube to 900V, the photomultiplier tube adopts two-stage amplification, the scanning voltage is 0-2.0V, the working electrode is a GC electrode, and the buffer system is 0.1mol / L phosphate buffer solution with pH=6.0. Select the concentration of n=8 dinuclear bipyridyl ruthenium metal complexes as 1×10 -4 mol / L, add levofloxacin to observe its influence on ECL, detect the luminescent signal of the solution, the results are as follows figure 2 shown. With the addition of levofloxacin, the ECL intensity gradually increased, and the concentration of levofloxacin ranged from 1 × 10 -14 ~1×10 -7 mol / L, the logarithm of the ECL luminous intensity change (ΔECL) and the logarithm of the concentration of levofloxacin showed a good linear relationship. The minimum detection limit of levofloxacin is 1×10 -14 mol / L, the linear correlation coefficient ...
Embodiment 3
[0053] Use the MPI-B type electrochemiluminescence analyzer (electrochemical workstation), set the high voltage of the photomultiplier tube to 900V, the photomultiplier tube adopts two-stage amplification, the scanning voltage is 0-2.0V, the working electrode is a GC electrode, and the buffer system is 0.1mol / L phosphate buffer solution with pH=7.0. Select the concentration of n=8 dinuclear bipyridyl ruthenium metal complexes as 1×10 -4 mol / L, add norfloxacin to observe its influence on ECL, detect the luminescent signal of the solution, the results are as follows image 3 shown. With the addition of norfloxacin, the ECL intensity gradually increased, and the concentration of norfloxacin ranged from 1×10 -15 ~1×10 -6 mol / L, the change value of ECL luminous intensity (ΔECL) and the logarithm of norfloxacin concentration showed a good linear relationship. The minimum detection limit of norfloxacin is 1×10 -15 mol / L, the linear correlation coefficient is 0.99469, and the con...
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