Fluoroglycofen-ethyl degradation pseudomonas YS-03 and application thereof

A technology of acifluorfen and YS-03, which is applied to acifluorfen-degrading Pseudomonas YS-03 and its application field, can solve problems such as residues, achieve low production and use costs, good removal effect, Ease of use

Active Publication Date: 2014-11-05
JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of fluorine-containing pesticides leads to the residue of such pesticides in the soil and water environment, which may pose a potential threat to the ecological environment and human health. Therefore, environmental behaviors such as the migration, transformation and decomposition of fluorine-containing pesticides in the environment are increasingly being focus on

Method used

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  • Fluoroglycofen-ethyl degradation pseudomonas YS-03 and application thereof
  • Fluoroglycofen-ethyl degradation pseudomonas YS-03 and application thereof
  • Fluoroglycofen-ethyl degradation pseudomonas YS-03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening and isolation of acifluorfen-degrading Pseudomonas YS-03

[0026] Get 5.0ml of activated sludge obtained from the wastewater treatment tank of a certain pesticide factory production workshop that produces acetoxyfen and add it into 100ml of inorganic salt liquid culture that the concentration of acetoxyfen is 100mg / L, and its formula is: Liter contains 1.50 g K 2 HPO 4 , 0.50 g KH 2 PO 4 , 0.20 g MgSO 4 ×7H 2 O, 1.00 g NaCl, 1.00 g (NH 4 ) 2 SO 4 , pH 7.0, cultured with shaking at 160r / min, 30°C, transferred to fresh inorganic salt medium at 5% inoculum every 5 days, and transferred 5 times in a row.

[0027] Take 1.0ml of the enriched bacteria solution obtained above, add 9.0ml of sterile water, and make 10 -1 The rich solution, and then draw 1.0ml prepared 10 -1 The enrichment solution was added to 9.0ml sterile water, mixed thoroughly to make 10 -2 The enrichment solution, and so on, carry out gradient dilution on the enrichment solutio...

Embodiment 2

[0029] Example 2 Shake Flask Degradation Experiment of Pseudomonas YS-03

[0030] In the inorganic salt medium containing 100 mg / L acifluorfen, the formula is: 1.50 g K per liter 2 HPO 4 , 0.50g KH 2 PO 4 , 0.20g MgSO 4 ×7H 2 O, 1.00g NaCl, 1.00g (NH 4 ) 2 SO 4 , pH 7.0, inoculate YS-03 according to 5% inoculum size, culture with shaking at 30°C, and take samples for measurement after 72 hours. Compared with the blank, the absorption peak of the treated sample decreased significantly, and acifluorfen was degraded by more than 90%.

Embodiment 3

[0031] Embodiment 3 Contains the preparation of Pseudomonas YS-03 bacterial agent

[0032] The original species of Pseudomonas acifluorfen-degrading Pseudomonas YS-03 screened and isolated in Example 1 were activated on a petri dish, and the degradation performance was measured, and inoculated on the inclined surface of the test tube for later use. The test tube was inoculated in a 1000ml shake flask containing 200ml LB medium. The formula of LB medium was: yeast extract 5.00g / L, peptone 10.00g / L, NaCl 10.00g / L, pH 7.0, constant temperature shaking culture to logarithmic phase , ready to inoculate the seed pot. The seed tank is 500 liters, the feeding volume is 400 liters, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask ...

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Abstract

The invention provides a degrading bacterial agent for eliminating fluoroglycofen-ethyl residue in wastewater, and belongs to the high technical field of biology. Used strains are Gram staining reaction negative bacteria YS3, and identified as pseudomonas (Pseudomonas sp.). Main biological characteristics are that the fluoroglycofen-ethyl degradation pseudomonas YS-03 is G-, rod-shaped, and free of spores, and has a polarflagella of which the size is 0.8 to 1.3*0.5 to 0.6mu m; catalase is positive; oxidase is negative; starch cannot be hydrolyzed, gelatin cannot be liquefied, the voges-proskauer test reaction is positive, the methyl red negative reaction is negative, indole is not produced, and Tween-80 is hydrolyzed. The pesticide residue in water can be lowered by more than 90% by direct application of a degrading bacteria product.

Description

Technical field [0001] The present invention belongs to the high-tech field of biology. It is a method of degrading chemical pesticides by using microorganisms. It is suitable for the production and processing of green-free agricultural products in modern agricultural production.Its application. Background technique [0002] Fluorine -containing pesticides are emerging pesticides used in recent decades.The fluorine atoms have the characteristics of small radius and large electrical negativeness. Introducing fluorine atoms and fluoride groups (such as: -CF3) into organic compounds with biological activity can greatly improve the stability and physiological activity of organic fluorine.As a hot spot for research and development today.However, the large amount of fluorine -containing pesticides caused the residues of such pesticides in the soil and water environment, which may pose a potential threat to the ecological environment and human health. Thereforefocus on. [0003] With th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/38A62D101/04A62D101/28
Inventor 张永乐陈建中张金
Owner JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
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