Kit and method for detecting human leucocyte antigen HLA-B*1502 genetype

A technology of leukocyte antigen and HLA-B, which is applied in the field of kits for detecting the genotype of human leukocyte antigen HLA-B*1502, can solve the problems of many sequencing methods, many steps, expensive chip method equipment, etc., and achieve good specificity Effect with sensitivity, easy operation
CN103114130BInactive Publication Date: 2014-07-09瀚吉康生物科技(北京)有限公司
3 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
瀚吉康生物科技(北京)有限公司
Publication Date
2014-07-09
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a kit and a method for detecting human leucocyte antigen HLA-B*1502 genetype. The kit disclosed by the invention is characterized by comprising the following primer sequences: a forward primer FP: 5'-CGACGCCGCGAGTCCCAGG-3'; and a reverse primer RP: 5'-CGTCGTAGGCGGACTGGTCATA-3'. The kit further comprises the following probe sequence: a probe PR: 5'-Cy5-AACACACAGATCTCCAAGACCAACACAC-p-3'. The kit disclosed by the invention has good specificity and sensitivity for carrying out PCR (Polymerase Chain Reaction) detection of the human leucocyte antigen HLA-B*1502. The PCR detection method disclosed by the invention has the advantages of being simple for operation (closed pipe), rapid, accurate and the like and is applied to clinical molecular diagnosis.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biology, in particular to a kit and a method for detecting the genotype of human leukocyte antigen HLA-B*1502. Background technique

[0002] Carbamazepine is a tricyclic anticonvulsant widely used in the treatment of epilepsy, trigeminal neuralgia, and manic depression. As the drug is widely used, its adverse reactions are increasingly prominent. The milder adverse reactions are maculopapular exanthema-MPE, including Stevens-Johnson syndrome-SJS, Toxic epidermal necrolysis-TEN, drug hypersensitivity syndrome(HSS) Other serious adverse reactions can be life-threatening.

[0003] Clinical pharmacogenomics studies have linked carbamazepine-induced SJS-TEN to human leukocyte antigen HLA-B*1502 genotype. In recent years, pharmacogenomics has covered different countries and regions in Asia, such as Taiwan, Hong Kong, Thailand, South Korea, Japan, and China. These studies found that 94.1-100% of patients with SJS-TEN ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More