Antrodiella zonata and bacterial reagent made from same for degrading metalaxyl pesticide residue
A thin-porous bacteria and annulus technology is applied in the field of environmental microorganisms to achieve the effects of not easy to mutate, reducing human toxicity and protecting the ecological environment
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[0030] 3. Preparation of straw section culture: Cut the straw into sections with a length of about 5 mm, add distilled water to make the water content of the straw section 50-60% (W / W), and then sterilize at 121°C for 20-30 minutes.
[0031] 4. Straw segment inoculation and solid aerated fermentation: Inoculate the straw segment with the homogenized mycelium inoculum solution, the inoculation amount is 10-15% (W / W); after the inoculation is completed, mix well, and put it into a 40cm x 25cm In a ×5cm fermentation tray, the total volume of straw segments is 3.5-4L. Put the rice straw inoculum in an incubator for solid-state aeration fermentation, the sterile air ventilation rate is 0.8-1 (V / V min), and the culture temperature is 30-32°C; after 15-18 days of culture, the solid-state fermentation is completed, and the fermented product is taken out and stirred Evenly.
[0032] The bacteria agent produced by the technical scheme of the invention can effectively degrade metalaxyl ...
Embodiment 1
[0034] Cultivation of strains: Pick up the hyphae of Antrodiella zonata D7821 with an inoculation needle and inoculate them into the plate of the culture medium for the separation and preservation of the above strains, and cultivate them in a 30°C incubator for 4 days. The colonies are loose and snowy. White, cottony. Scrape the loose hyphae with a scalpel and inoculate them into a conical flask filled with 500ml of liquid medium that was autoclaved at 121°C and 0.1MPa for 20min. Then placed at 30° C., rotating at 140 rpm / min, and cultured on a shaker for 5 days to obtain mycelial balls and culture solution.
[0035] Straw solid-state fermentation: Dried straw is chopped, and the straw section is about 5mm long. Take 1kg and put it into a PE box with a cover, add 600g of water, stir evenly, and sterilize at 121°C for 20-30 minutes. Take out the strain cultured by the above liquid, and homogenize with an in-cut homogenizer at a rotation speed of 5000rmp / min for 30 seconds. Ta...
Embodiment 2
[0036] Example 2 Degradation of metalaxyl pesticide residues by solid-state fermentation degrading bacteria agent
[0037] (1) Take 3kg of protected vegetable field soil with a metalaxyl content of 5mg / kg, a soil moisture content of 60%, and a hole diameter of 1mm 2 Nylon mesh screen removes large stones and sundries;
[0038] (2) Take about 1.5kg of the solid-state fermentation and degradation bacterial agent of the straw section, add it to the soil, stir and mix well, spread the mixture with a thickness of 5cm, and cultivate it at 30°C for 18 days. Spray water mist once every 2 days to maintain the humidity of the mixture at about 60%. The non-inoculated rice straw and the soil containing the equal concentration of metalaxyl pesticide were used as the control group.
[0039] (3) Take a sample every 3 days, take 3g of soil samples and place them in a 50mL plastic centrifuge tube, add 10mL of acetonitrile, shake on an oscillator for 30min to fully extract (model: QL-861 and fr...
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