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Antrodiella zonata and bacterial reagent made from same for degrading metalaxyl pesticide residue

A thin-porous bacteria and annulus technology is applied in the field of environmental microorganisms to achieve the effects of not easy to mutate, reducing human toxicity and protecting the ecological environment

Active Publication Date: 2013-05-29
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the continuous planting of vegetables and fruits, the high frequency of use of protected soil has caused soil fertility to decline, and various plant pathogens have grown in large numbers in confined spaces. In order to inhibit these pathogens, various types of fungicides must be used at higher concentrations, which results in chemical pesticides in the soil. persistent accumulation of residues
At present, there is no literature report on the degradation of metalaxyl pesticide residues by the use of Microporosus annulus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] 3. Preparation of straw section culture: Cut the straw into sections with a length of about 5 mm, add distilled water to make the water content of the straw section 50-60% (W / W), and then sterilize at 121°C for 20-30 minutes.

[0031] 4. Straw segment inoculation and solid aerated fermentation: Inoculate the straw segment with the homogenized mycelium inoculum solution, the inoculation amount is 10-15% (W / W); after the inoculation is completed, mix well, and put it into a 40cm x 25cm In a ×5cm fermentation tray, the total volume of straw segments is 3.5-4L. Put the rice straw inoculum in an incubator for solid-state aeration fermentation, the sterile air ventilation rate is 0.8-1 (V / V min), and the culture temperature is 30-32°C; after 15-18 days of culture, the solid-state fermentation is completed, and the fermented product is taken out and stirred Evenly.

[0032] The bacteria agent produced by the technical scheme of the invention can effectively degrade metalaxyl ...

Embodiment 1

[0034] Cultivation of strains: Pick up the hyphae of Antrodiella zonata D7821 with an inoculation needle and inoculate them into the plate of the culture medium for the separation and preservation of the above strains, and cultivate them in a 30°C incubator for 4 days. The colonies are loose and snowy. White, cottony. Scrape the loose hyphae with a scalpel and inoculate them into a conical flask filled with 500ml of liquid medium that was autoclaved at 121°C and 0.1MPa for 20min. Then placed at 30° C., rotating at 140 rpm / min, and cultured on a shaker for 5 days to obtain mycelial balls and culture solution.

[0035] Straw solid-state fermentation: Dried straw is chopped, and the straw section is about 5mm long. Take 1kg and put it into a PE box with a cover, add 600g of water, stir evenly, and sterilize at 121°C for 20-30 minutes. Take out the strain cultured by the above liquid, and homogenize with an in-cut homogenizer at a rotation speed of 5000rmp / min for 30 seconds. Ta...

Embodiment 2

[0036] Example 2 Degradation of metalaxyl pesticide residues by solid-state fermentation degrading bacteria agent

[0037] (1) Take 3kg of protected vegetable field soil with a metalaxyl content of 5mg / kg, a soil moisture content of 60%, and a hole diameter of 1mm 2 Nylon mesh screen removes large stones and sundries;

[0038] (2) Take about 1.5kg of the solid-state fermentation and degradation bacterial agent of the straw section, add it to the soil, stir and mix well, spread the mixture with a thickness of 5cm, and cultivate it at 30°C for 18 days. Spray water mist once every 2 days to maintain the humidity of the mixture at about 60%. The non-inoculated rice straw and the soil containing the equal concentration of metalaxyl pesticide were used as the control group.

[0039] (3) Take a sample every 3 days, take 3g of soil samples and place them in a 50mL plastic centrifuge tube, add 10mL of acetonitrile, shake on an oscillator for 30min to fully extract (model: QL-861 and fr...

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Abstract

The invention provides antrodiella zonata and a bacterial reagent made from antrodiella zonata for degrading metalaxyl pesticide residue. The strain is a white corroded fungus and is identified as antrodiella zonata D7821 which belongs to bracket fungus, Basidiomycetes. The strain is collected and separated from broad-leaved tree rottenwood of a primeval forest, and the strain has the main cultivating characteristic that the bacterial colony is loose, flocculent and white. ITS sequence of the strain is submitted to GenBank, with the register number of KC218571. Degrading bacteria cultivated by liquid and fermented by straw in solid state are applied to soil of vegetable fields in protected land polluted by metalaxyl pesticide, and the pesticide residue in the soil within 18 days can be reduced by over 75%. As the bacterial reagent for degrading metalaxyl pesticide residue, produced according to the technical scheme can effectively repair soil of the vegetable fields in the protected land polluted by pesticide, the toxicity of pesticide residue to a human body is reduced, and the ecological environment is protected.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, uses microorganisms to degrade chemical pesticide residues in the soil of protected vegetable fields, and relates to a oomycete fungicide—metalaxyl, which is isolated from rotting wood in primitive forests and has a high-efficiency degradation effect. Antrodiella zonata strain. technical background [0002] With the mass production and widespread use of pesticides, while bringing huge agricultural benefits to people, it has also caused serious environmental pollution. The serious threat of chemical pesticides to human health has also been paid more and more attention by people. Some of the pesticides applied to crops are attached to the crops, some are scattered in the soil, air and water environment, and some of the pesticides remaining in the environment will be absorbed by plants. Pesticide residues directly reach humans and animals through plant fruits, water, and air, or are fin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10C12R1/645
Inventor 袁海生戴玉成魏玉莲
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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