Method for extracting anthocyanidin from aria

A technology of anthocyanin and extraction solution, applied in the direction of organic chemistry, which can solve the problems of high process conditions, long extraction time, high equipment cost, etc., and achieve the effect of simple and easy operation, short extraction time and simple equipment

Inactive Publication Date: 2013-06-12
苏州鲜活饮品股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extraction process conditions of blueberry anthocyanins [J], Journal of Dalian Institute of Light Industry, 2007, 26 (3): 196-198); the extraction time of water extraction is long and the efficiency is low
[0006] 2. Auxiliary extraction by other means: On the basis of solvent extraction, auxiliary means such as microwave, ultrasonic, high-voltage pulsed electric field, ultra-high pressure, etc. are added to assist extraction. The extraction rate of microwave-assisted extraction is low. Ultrasonic, ultra-high pressure, supercritical fluid , The extraction efficiency of high-voltage pulsed electric field-assisted extraction is high, but the equipment cost is high, the process conditions are high, and the anthocyanins in the obtained extract will be damaged due to process conditions and reduce the antioxidant capacity, which is difficult to apply in actual production
[0008] 4. Microbial fermentation or enzymatic hydrolysis: use microorganisms or enzymes to degrade the cell wall components, so that the intracellular anthocyanins permeate and diffuse, and extract. This method uses acidified water to protect anthocyanins from degradation or acylation to make them more stable. But it takes a long time, so far it is still in the stage of laboratory operation, the stability is not good, and it has not yet been used to industrialization
[0009] As can be seen from the above description, although the extraction methods of anthocyanins can be seen in many documents, the domestic industry still uses organic solvent extraction as the main method, especially methanol and acetone with the lowest cost as solvents for extraction. It is very difficult to completely remove toxic solvents after extraction by such methods, and it is not easy to solve problems such as environmental pollution and health damage to operators due to the easy volatilization of toxic solvents, so it is indeed necessary to improve

Method used

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  • Method for extracting anthocyanidin from aria
  • Method for extracting anthocyanidin from aria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) 500g of oldberry is pulverized and beaten by a beater to obtain the oldberry slurry;

[0035] (2) Ethanol: distilled water (volume ratio) = 70:30, 0.1% citric acid and mix well as the extraction solvent; take 20g of bulangberry slurry and 300ml of extraction solvent, mix well, and use a full-temperature shaker at 30℃~33℃ for 250r / min vibration extraction 60min;

[0036] (3) After shaking and extracting, filter the mixture of slurry and extraction solvent, centrifuge at 4200r / min for 15min, and collect the supernatant as the extract;

[0037] (4) The extract is concentrated by vacuum rotary evaporation in a water bath at 40°C, and most of the ethanol is removed to obtain an anthocyanin concentrate;

[0038] (5) Measure the content of monomeric anthocyanins, and obtain 1.83g of monomeric anthocyanins per 100g of berry, and the extraction rate is 9.61%.

Embodiment 2

[0040] (1) 500g of oldberry is pulverized and beaten by a beater to obtain the oldberry slurry;

[0041] (2) Ethanol: distilled water (volume ratio) = 80:20, 0.1% citric acid and mix well as the extraction solvent; take 20g of berry slurry and 300ml of extraction solvent, mix well, and use a full-temperature shaker at 30℃~33℃ for 250r / min vibration extraction 60min;

[0042] (3) After shaking and extracting, filter the mixture of slurry and extraction solvent, centrifuge at 4200r / min for 15min, and collect the supernatant as the extract;

[0043] (4) The extract is concentrated by vacuum rotary evaporation under the condition of 42°C water bath, most of the ethanol is removed, and anthocyanin concentrate is obtained;

[0044] (5) Measure the content of monomeric anthocyanins, and obtain 1.90g of monomeric anthocyanins per 100g of berry, and the extraction rate is 9.99%.

Embodiment 3

[0046] (1) 500g of oldberry is pulverized and beaten by a beater to obtain the oldberry slurry;

[0047] (2) Ethanol: distilled water (volume ratio) = 90:10, and 0.1% citric acid are mixed evenly as the extraction solvent; take 20g of the berry slurry and 300ml of the extraction solvent, mix evenly, and shake with a full-temperature oscillator at 30°C to 33°C 250r / min extraction 60min;

[0048] (3) After shaking and extracting, filter the mixture of slurry and extraction solvent, centrifuge at 4200r / min for 15min, and collect the supernatant as the extract;

[0049] (4) The extract is concentrated by vacuum rotary evaporation under the condition of 42°C water bath, most of the ethanol is removed, and anthocyanin concentrate is obtained;

[0050] (5) Measure the content of monomeric anthocyanins, and obtain 1.85g of monomeric anthocyanins per 100g of berry, and the extraction rate is 9.71%.

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Abstract

The invention discloses a method for extracting anthocyanidin from aria. The method is characterized by comprising the following steps of: smashing and pulping the aria to obtain aria pulp; preparing an extraction solvent by using ethanol and distilled water; uniformly mixing the aria pulp and the extraction solvent according to a proper proportion and oscillating by using an oscillator to extract; after oscillating extraction, filtering and centrifuging a mixture of the pulp and the extraction solvent to obtain extract; and performing decompressed concentration on the extract to removal most of ethanol so as to obtain anthocyanidin concentrate. The method is quick and simple, and is environment-friendly since no toxic solvent is adopted for extraction; the obtained extract, monomer anthocyanidin, has high content and can be directly added into foods.

Description

technical field [0001] The invention relates to a method for extracting anthocyanins, in particular to a method for extracting anthocyanins from berry. Background technique [0002] Anthocyanidin, also known as anthocyanidin, is a kind of water-soluble natural pigment widely present in plants in nature, belonging to flavonoid compounds. At present, most of the pigments used in the food industry are synthetic pigments, almost all of which are toxic to varying degrees. Long-term use will endanger human health. Therefore, natural pigments have attracted more and more attention in the field of scientific research. Therefore, the extraction of high-purity anthocyanins provides the necessary characterization conditions and theoretical basis for the in-depth research and development of anthocyanin pigments, and is helpful for its industrial utilization. [0003] The scientific name of the old berry belongs to the Rosaceae (Roseaceae) Sorbus (Sorbus) (Aria), mainly grown in North A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
Inventor 黄国晃
Owner 苏州鲜活饮品股份有限公司
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