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Preparation method of blueberry anthocyanin and application of blueberry anthocyanin prepared

A technology of blueberry anthocyanin and blueberry powder, which is applied in the preparation of blueberry anthocyanin, blueberry anthocyanin, and the application field of blueberry anthocyanin. wide range of effects

Inactive Publication Date: 2013-06-12
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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[0005] Through the search, no patent publications related to this patent application were found

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  • Preparation method of blueberry anthocyanin and application of blueberry anthocyanin prepared
  • Preparation method of blueberry anthocyanin and application of blueberry anthocyanin prepared
  • Preparation method of blueberry anthocyanin and application of blueberry anthocyanin prepared

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Embodiment Construction

[0030] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0031] The main idea of ​​the present invention is: use blueberry as raw material, extract blueberry anthocyanin extract liquid by organic solvent extraction method, then obtain blueberry anthocyanin after purification, apply the obtained blueberry anthocyanin to human liver cancer cell HepG2 cells, observe blueberry flower color Protective effect of glycosides on DNA damage in human hepatocellular carcinoma cells induced by ultraviolet irradiation.

[0032] The invention uses blueberry anthocyanin to treat human liver cancer cell HepG2, and observes the protective effect of blueberry anthocyanin on human liver cancer cell DNA damage caused by ultraviolet radiation. Cell experiments showed that compared with the ultraviolet irradiation group...

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Abstract

The invention relates to a preparation method of blueberry anthocyanin. The method comprises the following steps: blueberries are freeze-dried, mashed and screened, blueberry powder and ethanol are mixed according to a powder to liquor ratio g / g of 1:8-12, the mixture is extracted and filtered at the condition of the temperature of 85 DEG C, the step is repeated for twice and filtrate is combined so that primary extraction liquid of the blueberry anthocyanin is obtained; and the primary extraction liquid is centrifuged for 10min at a rate of 3000r / min, supernate is filtered in a mode of suction, the filtrate is concentrated, purified and ethanol-eluted so that eluent is obtained, and the eluent is extracted and vacuum freeze dried so that the blueberry anthocyanin is obtained. The blueberry anthocyanin primary extraction liquid is obtained through an organic solvent extraction method, and is subjected to centrifugation, suction filtering, concentration, resin purification, extraction by petroleum ether and vacuum freeze drying so that a blueberry anthocyanin sample is obtained. Preparation method is simple and interest rate of the blueberry anthocyanin is high.

Description

technical field [0001] The invention belongs to the field of nutrition and immunity, and relates to blueberry anthocyanins, in particular to a preparation method of blueberry anthocyanins and an application of the prepared blueberry anthocyanins. Background technique [0002] With the destruction of the atmospheric ozone layer and changes in human lifestyles, the increase in sunlight exposure has significantly increased the incidence of skin cancer. After ultraviolet rays act on cells, their photon energy is absorbed by atoms or molecules, causing changes in the electronic energy levels of molecular atoms, which in turn lead to related events in cells, such as cellular DNA damage, changes in gene expression, inhibition of cell cycle progression, DNA -Protein cross-linking and chromosomal aberration; affect and inhibit skin immune function, making mutant cells easily escape the body's immune surveillance, thus easily leading to the occurrence of skin squamous cell carcinoma a...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08A61P35/00
Inventor 刘薇卢相义罗成
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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