A kind of method and application of preparing compound lactic acid bacteria preparation

A technology of compounding lactic acid bacteria and lactic acid bacteria is applied in the fields of microbiology and feed additives, which can solve the problems of resource waste and environmental pollution, and achieve the effects of cost saving, easy digestion and absorption, and reduction of water content.

Active Publication Date: 2015-08-19
GUANGDONG YIHAO FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain competitiveness of the lactic acid bacteria preparation obtained by the method is enhanced, and the host colonization rate is improved. Moreover, the method can effectively utilize the waste residue resources of agricultural and sideline products, making it an important part of the feed additive composition, and solves the resource problem to a certain extent. Outstanding issues such as waste and environmental pollution

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  • A kind of method and application of preparing compound lactic acid bacteria preparation
  • A kind of method and application of preparing compound lactic acid bacteria preparation
  • A kind of method and application of preparing compound lactic acid bacteria preparation

Examples

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Effect test

Embodiment 1

[0036] A preparation method of a compound lactic acid bacteria preparation, the compound lactic acid bacteria are Lactobacillus casei and Lactobacillus acidophilus, and the mixing ratio is 1:1.

[0037] A kind of preparation method step of compound lactic acid bacteria preparation comprises:

[0038] S1. Strain expansion culture.

[0039] S11. Rejuvenation of Lactobacillus casei and Lactobacillus acidophilus slant: the corresponding colonies were picked from the preserved slant strains for expansion and rejuvenation, inoculated on MRS medium respectively, and cultured at 35°C for 72 hours to make them in the middle and late logarithmic stages. get rejuvenated strains;

[0040] Wherein, the medium described in S11 is MRS slant solid medium.

[0041] S12. Cultivation of lactic acid bacteria casei and Lactobacillus acidophilus on a shaking table: wash the rejuvenated lactic acid bacteria strains in step S11 with sterile water to make a bacterial suspension, inoculate them i...

Embodiment 2

[0067] A preparation method for a compound lactic acid bacteria preparation, the compound lactic acid bacteria species are 5 kinds of strains of Lactobacillus casei, Lactobacillus acidophilus, Streptococcus thermophilus, Enterococcus faecium and Lactobacillus plantarum, and the composition ratio is 1:1:1:1: 1.

[0068] A kind of preparation method step of compound lactic acid bacteria preparation comprises:

[0069] S1. Strain expansion culture

[0070] S11. Lactobacillus slant rejuvenation: Pick corresponding colonies from the preserved slant strains, expand culture and rejuvenate them, inoculate them on MRS medium respectively, and culture them at 40°C for 48 hours to make them in the middle and late logarithmic stages to obtain rejuvenated strains;

[0071] Wherein, the medium is MRS slant solid medium.

[0072] S12. Cultivation of lactic acid bacteria casei and Lactobacillus acidophilus on a shaking table: wash the rejuvenated lactic acid bacteria strain...

Embodiment 4

[0120] In the experiment, 200 Binary No. 1 black pigs with a body weight of 31-32kg were selected and randomly divided into 4 groups (control group 1, control group 2, test group 1 and test group 2). There was no significant difference in average body weight (P>0.05). Two Yuan No. 1 black pigs were provided by Guangdong No. 1 Food Co., Ltd., and the test site was carried out in the third line of the fattening area of ​​Suixi Guanhu Base, Zhanjiang, Guangdong.

[0121] What the control group 1 fed was the pig daily eclipse feed; what the control group 2 fed was the mixture of the compound lactic acid bacteria preparation prepared in the comparative example 3 of the present invention and the pig daily eclipse feed; what the test group 1 fed was the preparation of the embodiment 1 of the present invention The mixture of the compound lactic acid bacteria preparation and the pig daily eclipse feed; what the test group 2 fed was the mixture of the compound lactic acid bacteri...

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Abstract

The invention belongs to the technical field of animal feeds and in particular discloses a method for preparing a compound lactobacillus preparation and the application of the compound lactobacillus preparation. The method comprises the steps of: expanded culturing of strains, liquid culturing of compound strain, waste residue pretreating of agricultural and sideline products and liquid-solid coupling fermentation of compound strain. According to the method for preparing compound lactobacillus preparation, the problem that the existing probiotic feed additives are poor in performance stability and high in production cost is overcome, the competitiveness of the strain in compound lactobacillus preparation product is increased, the host colonization rate is improved, the cost is greatly reduced, and the waste residue resource of agricultural and sideline products can be effectively utilized to serve as an important component of the feed additive, so that the prominent problems of wasting of resources and environmental pollution can be partly solved; and therefore, the compound lactobacillus preparation can be successfully applied to the aspect of feeds.

Description

technical field [0001] The invention relates to the technical fields of microbiology and feed additives, and more specifically relates to a method and application for preparing a compound lactic acid bacteria preparation. Background technique [0002] Probiotic preparation (microecological preparation) is a kind of green feed that is safe, non-toxic and side effects, can promote the growth of livestock and poultry, improve the meat quality and flavor of livestock products, and is beneficial to the ecological environment and export trade based on the safety of feed antibiotics. additive. Lactic acid bacteria are important strains in probiotic preparations. Most of them are anaerobic or facultative anaerobic Gram-positive bacteria that grow in an environment rich in mineral and organic nutrients. They are acid-resistant and pH 3.0~ It can still grow at 4.5 and adapt to the acidic environment of the gastrointestinal tract; [0003] As we all know, probiotics produced by indus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16A23K1/18C12R1/245C12R1/23C12R1/46C12R1/25
Inventor 黎小兵胡杰杨星江彬强黄升科
Owner GUANGDONG YIHAO FOOD
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