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Compound biological flocculant and preparation method thereof

A microbial flocculant and composite technology, applied in the field of microorganisms, can solve the problem of not jointly preparing microbial flocculants, etc., and achieve the effects of good application prospects, simple process and low cost.

Inactive Publication Date: 2015-04-15
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the preparation of microbial flocculants by Serratia marcescens
There are also no studies on the joint production of microbial flocculants by Serratia marcescens and other species

Method used

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  • Compound biological flocculant and preparation method thereof
  • Compound biological flocculant and preparation method thereof
  • Compound biological flocculant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0042] Implementation Case 1 Screening and identification of high-efficiency flocculation bacteria

[0043] Alternative microorganisms with flocculation can be selected from activated sludge (derived from the activated sludge of Chongqing Heishizi Food Waste Treatment Plant), landfill leachate (derived from the comprehensive wastewater of Chongqing Fuling Lameizi Mustard Plant)

[0044] In the middle screening, the isolated strains were respectively inserted into 250ml wide-mouth Erlenmeyer flasks containing 50ml of fermentation medium, placed in a shaking table at 30°C and 200rpm for 24h, and the fermentation broth was taken for flocculation activity testing. figure 1 The flocculation activity test result of the bacterial strain obtained for screening, wherein Serratia marcescens (also known as phe-5) (biological deposit number is CTCC M 2013041) and Klebsiella (also known as ZC-7) (biological deposit number CCTCCM2013043) had the highest flocculation activity among the scree...

Embodiment example 3

[0050] Implementation Case 3 Preparation of Composite Microbial Flocculants

[0051] The flocculant-producing strains CTCC M2013041 and CCTCC M 2013043 with the best flocculation activity screened in Example 1 were respectively inserted into sterilized seed culture medium, and vibrated at 30°C and 220rpm for 24 hours to obtain Serratia marcescens respectively. Bacteria liquid and Klebsiella bacterium liquid, the raw material of described seed culture medium is made up of following ratio: glucose, 10.0g; K 2 HPO 4 , 5.0g; MgSO 4 .7H 2 O, 0.2g; KH 2 PO 4 , 2.0g; NaCl, 0.1g; urea, 0.5g; yeast powder, 0.5g, distilled water 1L.

[0052] Mix the Serratia marcescens bacterium liquid obtained in step A and or the Klebsiella bacterium liquid in a quantitative ratio of 1:1, inoculate it into the fermentation medium at an inoculum size of 1%, and culture it with shaking at 220 rpm for 24 hours at 30°C Finally, the thallus or bacterium liquid are microbial flocculants, and the raw m...

Embodiment 4

[0054] Example 4 Distribution of flocculation activity of composite microbial flocculants

[0055] Collect the composite microbial flocculant similar or equivalent to that prepared in Example 3, centrifuge at 5000rpm for 10min, collect the fermentation broth and bacteria respectively, wash the bacteria cells twice with pure water, and then resuspend with pure water equal to the volume of the fermentation broth . According to Example 2, the flocculation activity of the fermentation broth, cell suspension and supernatant were measured respectively. The results show( figure 2 ), cell suspension (10 10 The flocculation activity of CFU / mL) is 67.9%, obviously higher than fermentation liquid (flocculation activity is 55.4%) and supernatant liquid (flocculation activity is 61.2%), shows that the composition of the flocculation activity that bacterial strain produces mainly exists in the bacterial cell sediment .

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Abstract

The invention belongs to the technical field of microorganisms, relates to a biological flocculant as well as a preparation method and an application thereof and particularly relates to the application of serratia marcescens and klebsiella combination in the preparation of the microorganism flocculant. The preparation method of the microorganism flocculant comprises the following steps of: respectively putting strains in a sterile seed medium for cultivation, putting seed cultures in a sterile fermentation medium to carry out fermentation cultivation for 18 hours, then carrying out centrifugal separation on fermentation liquor, taking thalli and precipitating to obtain the compound microorganism flocculant. The preparation method is simple in process and low in cost; the prepared flocculant has high activity, strong heat resistance, pH stability, no secondary pollution, suitability for large-scale production and good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a biological flocculant and its preparation method and application. Background technique [0002] Flocculants are widely used in the field of sewage treatment. Among them, the commonly used flocculants are polyaluminum chloride-based flocculants and polyacrylamide flocculants. Aluminum ions in drinking water can cause Alzheimer's disease, and acrylamide monomer has a strong neurotoxicity. toxicity. Therefore, it is necessary to develop new flocculants with high efficiency, safety and no secondary pollution. [0003] Microbial flocculants are a kind of high molecular biopolymers with flocculation function produced by microorganisms under specific culture conditions. Accelerated settling to achieve solid-liquid separation. From the perspective of the source and composition of microbial flocculants, it is safe, non-toxic, easily biodegradable, environmentally f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P1/04C12R1/43C12R1/22
Inventor 宋立岩乔婧唐薇黄亦存李斗尹雅洁
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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