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High-affinity tree-like boric acid functionalized magnetic microsphere as well as preparation method and application thereof

A technology of magnetic microspheres and dendrites, applied in the field of functional materials, can solve problems such as weak affinity, and achieve the effects of improved affinity, enhanced binding ability, and improved binding force

Inactive Publication Date: 2013-06-19
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of weak affinity of existing substituted boronic acids and their functionalized materials, the first purpose of the present invention is to provide a high-affinity dendritic boronic acid functionalized magnetic microspheres

Method used

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  • High-affinity tree-like boric acid functionalized magnetic microsphere as well as preparation method and application thereof
  • High-affinity tree-like boric acid functionalized magnetic microsphere as well as preparation method and application thereof
  • High-affinity tree-like boric acid functionalized magnetic microsphere as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Preparation of dendritic boronic acid-modified magnetic nanoparticles functionalized with 4-formylphenylboronic acid (fourth generation PAMAM)

[0037] 200mg amino functionalized magnetic Fe 3 o 4 Nanoparticles [see Chem.Eur.J., 2006, 12, 6341-6347] were dispersed in 40 mL of anhydrous methanol containing 5% (w / w) glutaraldehyde, and mechanically stirred at room temperature for 12 hours to obtain aldehyde groups Activated magnetic nanoparticles, the resulting aldehyde-activated magnetic nanoparticles were dispersed in 30 mL containing 0.5 g fourth-generation PAMAM dendrimers and 1% (w / w) NaCNBH 3 In anhydrous methanol, mechanically stirred at room temperature for 12 hours to obtain dendrimer-modified magnetic nanoparticles. The resulting dendrimer-modified magnetic nanoparticles were dispersed in 40 mL containing 10 mg / mL 4-formylphenylboronic acid and 1% (w / w) NaCNBH 3 mixed with anhydrous methanol solution, stirred and reacted at room temperature for 24 ...

Embodiment 2

[0038] Example 2: Preparation of boric acid-functionalized magnetic nanoparticles grafted with seventh-generation PAMAM dendrimers

[0039] The magnetic nanoparticles activated by the aminoaldehyde groups obtained in Example 1 were dispersed in 30mL containing 0.3g of the seventh generation PAMAM dendrimers and 1% (w / w) NaCNBH 3 In anhydrous methanol, mechanically stirred at room temperature for 12 hours to obtain dendrimer-modified magnetic nanoparticles. The resulting dendrimer-modified magnetic nanoparticles were dispersed in 40 mL containing 10 mg / mL 4-formylphenylboronic acid and 1% (w / w) NaCNBH 3 mixed with anhydrous methanol solution, stirred and reacted at room temperature for 24 hours to obtain dendritic boric acid functionalized magnetic nanoparticles.

Embodiment 3

[0040] Example 3: Preparation of dendritic boronic acid-modified magnetic nanoparticles functionalized with 4-carboxyphenylboronic acid

[0041] The dendrimer-modified magnetic nanoparticles obtained in Example 1 were dispersed in 50 mL of 0.1 mol / L 2-(N-morpholino) ethanesulfonic acid buffer solution (MES, pH 6.3), and 500 mg of 1-ethane Base-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and 1g N-hydroxysuccinimide (NHS), 500mg 4-carboxyphenylboronic acid were mixed, stirred and reacted at room temperature for 12 hours to obtain Magnetic nanoparticles functionalized with dendritic boronic acid.

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Abstract

The invention discloses a high-affinity tree-like boric acid functionalized magnetic microsphere as well as a preparation method and an application thereof. The surface of the microsphere contains high-density boric acid radicals, and the polyvalent cooperative binding can be generated for cis-dihydroxy enriched biological molecules, so that bonding force is obviously improved. The dissociation constant of the microsphere to glycoprotein is 10<-5> to 10<-6>M, the bonding force is improved by 3-4 orders of magnitude compared with the ordinary boric acid functionalized material, and the glycoprotein with the concentration of 2*10<-14>M can be enriched. Moreover, the microsphere further has the advantages of being high in anti-jamming capability, high in binding capacity, quick in binding / analyzing speed, and the like. Since the microsphere has magnetism, a sample can be conveniently processed by applying an external field. According to the high-affinity tree-like boric acid functionalized magnetic microsphere disclosed by the invention, the cis-dihydroxy enriched biological molecules with low abundance in a complicated sample can be conveniently and quickly enriched, purified and separated.

Description

technical field [0001] The invention relates to the field of functionalized materials, and also relates to the enrichment, purification and separation of cis-dihydroxy biomolecules. Background technique [0002] Cis-dihydroxy biomolecules are a class of compounds containing cis-dihydroxy structures, such as sugars, glycoproteins and glycopeptides, nucleosides and nucleotides, which are respectively glycomics, proteomics and metabolomics, etc. The main research object of the field. These cis-dihydroxy biomolecules have very important research value. For example, sugar plays an important role in the process of life activities and is the main source of energy required by all living organisms to maintain life activities. Many glycoproteins are clinical cancer biomarkers and therapeutic targets, such as alpha-fetoprotein, carcinoembryonic antigen, CA125, and prostate-specific antigen. However, the abundance of glycoproteins in samples is very low, and often accompanied by high-...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30B01J13/02B01D15/08C07K1/22G01N1/40
Inventor 刘震王合叶
Owner NANJING UNIV
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