Short-period tissue culture method of peanuts

Abstract

The invention provides a short-period tissue culture method of peanuts. The short-period tissue culture method comprises the following steps of: (1) disinfecting the surfaces of peanut seeds and soaking the seeds; (2) inoculating embryonic leaflets of the peanut seeds on a cluster-bud inducing medium; (3) cutting cluster buds or cluster-bud tissue blocks, inoculating the cluster buds or the cluster-bud tissue blocks on a first extending medium for culture and a second extending medium for alternative culture till seedlings of 2cm-3cm in length are obtained; (4) cutting the seedlings of 2cm-3cm in length and inoculating the cut seedlings on a rooting medium for culture till strong roots grow and complete plants are obtained; and (5) hardening the complete plants and transplanting the hardened complete plants onto vermiculite. The short-period tissue culture method provided by the invention is used for carrying out tissue culture on peanuts, rooted peanut tissue culture seedlings can be obtained in 15 weeks, and the period of tissue culture is greatly shortened, so that the breeding speed of the peanut tissue culture seedlings is enhanced; and the peanut tissue culture seedlings cultured by the method are high in regeneration rate and good in repeatability.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a peanut short-cycle tissue culture method. Background technique [0002] Peanut is an important economic crop and oil crop in the world. my country's peanut production ranks first in the world, and its planting area ranks second in the world. It plays a pivotal role in the world's peanut production. Traditional breeding can effectively improve the characteristics of peanuts in terms of yield and drought resistance, but genetic engineering breeding can more effectively retain and screen some beneficial purpose traits than traditional breeding methods. The genetic basis of peanut is weak, and the available effective resources are limited. The key to genetic transformation of peanut using genetic engineering technology is to establish an efficient receptor system, that is, to establish an efficient method for tissue culture of peanut plants. [0003] Peanut is a ki...

AI Technical Summary

Problems solved by technology

[0003] Peanut is a kind of large-grained leguminous plant. There have been reports on the successful in vitro regeneration of peanuts at home and abroad: McKently et al. used cotyledons with embryos and cotyledons without embryos as explants to induce clustered buds, and found that cotyledons with embryos were the most suitable explants. , grew into a complete plant after 258 days, but the process of obtaining explants in the above studies was complicated and required a large amount of seeds; Charleen and Venkatachala respectively used embryonic leaflets as explants, and successfully induced embryogenic callus through somatic embryo regeneration. tissue, but there is a problem of differentiation into plants; Mroginski et al. used immature leaflets of 3-5d seedling age as explants to induce clustered buds and obtained complete plants; Tiwari et al. used immature embryonic leaflets as explants to study the pre-culture time , medium and genotype on the regeneration of embryonic leaflets, the regeneration rate of the whole process can reach 80%, and it takes 4 months, but the above-mentioned regeneration system using embryonic leaflets as explants still has difficulty in plant formation and low repeatability At the same time, the problems of low regeneration frequency and long cycle have yet to be solved. At the same time, studies have shown that plant regeneration in vitro has a strong dependence on genotype

Images