Simple and convenient nanometer electrospining regionalization method
An electrospinning and regionalization technology, which is applied in textiles and papermaking, filament/thread forming, fiber processing, etc., can solve the problems of electrospinning that cannot be regionalized and low analysis throughput, so as to reduce screening costs, The effect of improving the sub-table rate and good isolation
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Embodiment 1
[0035] Example 1 PLGA (polylactic acid-glycolic acid copolymer) electrospinning regionalization
[0036] Dissolve PLGA in trifluoroethanol to make an 8% solution, and place it on a magnetic stirrer and stir for 1 hour before use. The PLGA solution was injected into a 1 mL syringe, the tip of the syringe needle was ground flat, and the positive electrode of the high-voltage power supply was connected. The clean glass is placed on the aluminum block connected to the high-voltage ground electrode. The distance from the syringe needle is 8cm, the high-voltage power supply output voltage is 8kV, the syringe pump flow rate is 0.4mL / h, the spinning time is 0.5 hours, and the spinning thickness is 40μm. Remove the glass and the spinning fiber on it, place it on the XY-axis two-dimensional electric translation table, inject SU8 glue into the syringe, and fix it on the Z-axis translation table, adjust the distance between the syringe needle and the electrospinning surface to be less than ...
Embodiment 2
[0037] Example 2: PS (polycarbonate) electrospinning regionalization
[0038] Dissolve PS in a mixed solution of THF and acetone (volume ratio 1:1), make a 12% solution, and place it on a magnetic stirrer and stir for 5 hours before use. The PS solution was injected into a 1 mL syringe, the tip of the syringe needle was ground flat, and the positive electrode of the high-voltage power supply was connected. The clean glass is placed on the aluminum block connected to the high-voltage ground electrode. The distance from the syringe needle is 5cm, the high-voltage power supply output voltage is 10kV, the syringe pump flow rate is 0.4mL / h, the spinning time is 0.5 hours, and the spinning thickness is 40μm. Remove the glass and the spinning fiber on it, place it on the XY-axis two-dimensional electric translation table, inject SU8 glue into the syringe, and fix it on the Z-axis translation table, adjust the distance between the syringe needle and the electrospinning surface to be les...
Embodiment 3
[0039] Example 3: Preparation of electrospinning array using regionalized electrospinning method for three-dimensional cell culture
[0040] Using the method used in Example 1, a regionalized electrospinning array for three-dimensional cell culture was prepared. The electrospinning after regionalization is divided into circular cell culture regions with a diameter of 3 mm. Because cell culture conditions are relatively harsh, it is necessary to characterize whether this method affects the properties of electrospinning before it is used in cell culture: image 3 with Figure 4 It can be seen that the chemical properties and surface morphology of the regionalized electrospinning prepared in this way have not changed. At the same time, due to the hydrophobicity of SU8, the solution in each culture tank can be well maintained without affecting other culture tanks. Figure 5 In order to add dyes to the inside of the regionalized electrospinning, it can be seen that no liquid oozes fro...
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