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Process for extracting heparin sodium from intestinal mucosa by alkaline protease method

A protease and intestinal mucosa technology, applied in the field of extracting heparin sodium from intestinal mucosa by alkaline protease method, can solve the problems of long production cycle, environmental pollution, high energy consumption, etc., to reduce waste discharge, benefit environmental protection, and increase yield. Effect

Inactive Publication Date: 2013-07-03
HANGZHOU LONGYANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The disadvantages of the traditional enzymatic hydrolysis production method of heparin sodium are: long production cycle, high energy consumption, low yield, 2800-3000 pig small intestines are needed to produce 100 million international units of heparin sodium crude product, the product purity is low, and the titer of heparin sodium At most 80U / mg, and produce a large amount of intestinal residue and waste water, polluting the environment
The defect that this method exists is: product yield, purity are also lower, and produce a large amount of intestinal residues and waste water, pollute the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Enzymolysis: Add 2000L of porcine small intestinal mucosal slurry (obtained from pig small intestines treated by an intestinal scraper) into the enzymatic hydrolysis tank. The porcine small intestinal mucosal slurry is a mixture of porcine small intestinal mucosa: water = 1: 2.5 by weight, and add salt to the enzymatic hydrolysis tank Sodium chloride solution with a temperature of 21° adjusts the salinity of the feed liquid in the enzymolysis tank to 5±0.5°, and controls the pH of the feed liquid to be around 8; then add 0.8kg of enzyme to the enzymolysis tank for every 1000L feed liquid Add 2709 alkaline protease, control the feed solution temperature at 56°C, salinity 4.2±0.2°, pH 6.5 for 2.5 hours; finally raise the temperature to 85°C, keep it for 25 minutes to obtain the enzymatic solution, filter to remove the residue; then enzymatic solution at 6000 Under the rotating speed of rpm, centrifuge for 40min, and then enter the next step of adsorption.

[0042] Co...

Embodiment 2

[0052] (1) Enzymolysis: Add 1000L of porcine small intestine mucosal slurry (obtained from pig small intestines treated with an intestinal scraper) into the enzymatic hydrolysis tank. The porcine small intestinal mucosal slurry is a mixture of porcine small intestinal mucosa: water = 1: 4 by weight, and add salt to the enzymatic hydrolysis tank Sodium chloride solution with a temperature of 24° adjusts the salinity of the feed liquid in the enzymolysis tank to 5±0.5°, and controls the pH of the feed liquid to be around 9; then add 1.6kg of enzyme to the enzymolysis tank for every 1000L feed liquid Add 2709 alkaline protease, control the temperature of the feed solution at 50°C, salinity 4.2±0.2°, pH 7.5 and keep it for 3.5 hours; finally raise the temperature to 88°C, keep it for 20min to obtain the enzymolysis solution, filter to remove the residue; then the enzymolysis solution Under the rotating speed of 8000 rpm, centrifuge for 20 minutes, and then enter the next step of a...

Embodiment 3

[0063] (1) Enzymolysis: Add 1500L of porcine small intestinal mucosal slurry (obtained from pig small intestines treated by an intestinal scraper) into the enzymatic hydrolysis tank. The porcine small intestinal mucosal slurry is a mixture of porcine small intestinal mucosa: water = 1:3 by weight, and add salt to the enzymatic hydrolysis tank Sodium chloride solution with a temperature of 22° adjusts the salinity of the feed liquid in the enzymolysis tank to 5±0.5°, and controls the pH of the feed liquid at about 8.5; then add 1.2kg of enzyme to the enzymolysis tank for every 1000L feed liquid Add 2709 alkaline protease, control the feed liquid temperature at 53°C, salinity 4.2±0.2°, pH 7.0 and keep it for 3 hours; finally raise the temperature to 86°C, keep it for 22min to obtain the enzymatic solution, filter to remove the residue; then the enzymatic solution is in At a speed of 7000 rpm, centrifuge for 30 minutes before entering the next step of adsorption.

[0064]Collect...

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Abstract

The invention relates to the technical field of production of heparin sodium and provides a process for extracting heparin sodium from an intestinal mucosa by an alkaline protease method. The process mainly comprises the steps of performing enzymolysis, adsorbing, washing, eluting, precipitating and drying. According to the process, the production cycle is short, the products have high yield and purity, discharge of intestinal residue and waste water is reduced, and environmental protection is promoted. 100 million international units of heparin sodium crude products can be produced only by about 1,600 small intestines of pigs, and the valence of the heparin sodium is more than 110 U / mg.

Description

technical field [0001] The invention relates to the technical field of heparin sodium production, in particular to a process for extracting heparin sodium from intestinal mucosa with alkaline protease. Background technique [0002] Heparin sodium, also known as heparin, is a natural anticoagulant substance containing acidic mucopolysaccharides containing sulfate groups. Heparin sodium is white or off-white powder, odorless, tasteless, hygroscopic, soluble in water, insoluble in organic solvents such as ethanol, acetone, and dioxane. [0003] Heparin sodium widely exists in mammalian liver, lung, and intestinal mucosa, and mostly exists in complexes combined with proteins. Enzyme hydrolyzed protein can separate heparin sodium, which is mucopolysaccharide containing sulfuric acid, amino group and aldonic acid. At pH 8-9, it is negatively charged, and can perform ion exchange with anion exchangers for rough separation, and the polysaccharide solution is precipitated in high-c...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 花瑞华潘为群潘为中
Owner HANGZHOU LONGYANG BIOTECH
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