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Application of sulfonated lignin in wood fibre material enzyme hydrolysis saccharification

A technology for lignocellulosic raw material and sulfonated lignin, which is applied in the field of enzyme engineering, can solve the problems of increasing additional costs and the like, and achieves the effects of reducing the amount of enzymes, easy preparation and easy acquisition.

Inactive Publication Date: 2013-07-10
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, adding additives in the enzymatic hydrolysis process will undoubtedly increase additional costs, so additives with low cost, abundant sources, easy access, easy preparation, and high efficiency have more application prospects

Method used

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  • Application of sulfonated lignin in wood fibre material enzyme hydrolysis saccharification
  • Application of sulfonated lignin in wood fibre material enzyme hydrolysis saccharification
  • Application of sulfonated lignin in wood fibre material enzyme hydrolysis saccharification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: Pretreatment and enzymatic hydrolysis method using coniferous wood as raw material.

[0054] raw material:

[0055] The coniferous wood used in the experiment was produced in Fujian and provided by a paper mill in Fujian. The wood chips are about 1.5cm long, 2.5cm wide and 0.3cm thick. After air drying, balance the moisture for later use.

[0056] The mixed enzyme solution for enzymatic hydrolysis is composed of cellulase, xylanase and β-cellobiase. The enzyme activity ratio per unit volume of the three enzymes is cellulase: xylanase: β-cellobiase = 1FPU: 1.2FXU: 1CBU. The enzyme activity of the mixed enzyme solution is 20 FPU / ml based on the enzyme activity of cellulase filter paper.

[0057] Green liquor pretreatment process:

[0058] Green liquor comes from the alkali recovery system of kraft pulping and is mainly composed of sodium carbonate and sodium sulfide. In a self-made 10×1L oil bath, weigh 150g air-dried wood chips equivalent to absolute ...

Embodiment 2

[0074] Embodiment 2: Pretreatment and enzymatic hydrolysis method using hardwood as raw material.

[0075] raw material:

[0076] The hardwood used in the experiment was produced in northern Jiangsu. The wood chips are about 1.5cm long, 2.5cm wide and 0.2cm thick. After air drying, balance the moisture for later use.

[0077] The mixed enzyme solution for enzymatic hydrolysis is prepared by mixing cellulase, xylanase and β-cellobiase. The enzyme activity ratio per unit volume of the three enzymes is cellulase: xylanase: β-cellobiase = 1FPU: 1.2FXU: 1CBU. The enzyme activity of the mixed enzyme solution is 20 FPU / ml based on the enzyme activity of cellulase filter paper.

[0078] Green liquor pretreatment process:

[0079] Green liquor comes from the alkali recovery system of kraft pulping and is mainly composed of sodium carbonate and sodium sulfide. In a self-made 10×1L oil bath, weigh 150g air-dried wood chips equivalent to absolute dryness in each tank, add the cookin...

Embodiment 3

[0093] Embodiment 3: Pretreatment and enzymatic hydrolysis method using gramineous plants as raw materials.

[0094] raw material:

[0095] The gramineous plant Nandi used in the experiment was produced in Hunan. Cut the ingredients to about 3cm in length, air-dry and balance the moisture for later use.

[0096] The mixed enzyme solution for enzymatic hydrolysis is prepared by mixing cellulase, xylanase and β-cellobiase. The enzyme activity ratio per unit volume of the three enzymes is cellulase: xylanase: β-cellobiase = 1FPU: 1.2FXU: 1CBU. The enzyme activity of the mixed enzyme solution is 20 FPU / ml based on the enzyme activity of cellulase filter paper.

[0097] Green liquor pretreatment process:

[0098] Green liquor comes from the alkali recovery system of kraft pulping and is mainly composed of sodium carbonate and sodium sulfide. In a self-made 10×1L oil bath, weigh 100g of air-dried wood chips equivalent to dryness in each tank, add the cooking liquid with a solid...

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Abstract

The present invention discloses application of sulfonated lignin in wood fibre material enzyme hydrolysis saccharification. According to the invention, the sulfonated lignin is added in the enzyme hydrolysis process. The efficiency of enzyme hydrolysis saccharification can be improved, the retention time of the enzyme hydrolysis is shortened, also the amount of enzyme can be reduced, the enzyme activity is improved, and the enzyme hydrolysis cost of production of biological energy source from the wood fibre material is reduced.

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering, and in particular relates to the application of sulfonated lignin in enzymatic hydrolysis and saccharification of lignocellulosic raw materials. Background technique [0002] The depletion of fossil energy is seriously restricting the development and progress of world civilization, and the search for alternative renewable resources has become the research focus of countries all over the world. Since the 1970s, bioenergy, as a renewable potential energy source, has been favored by researchers from all over the world. At the same time, the application of bioenergy can also reduce the greenhouse gas CO produced by the use of traditional fossil energy. 2 And a small amount of harmful gases containing sulfur and nitrogen, which can effectively alleviate the greenhouse effect. As a second-generation biofuel, lignocellulosic raw materials have the characteristics of large output and low cos...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 金永灿王旺霞谷峰杜晶
Owner NANJING FORESTRY UNIV
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