Method for preparing collagen protein powder from fish scales

A technology of collagen powder and fish scales, which is applied in fish protein components, protein food components, food science, etc., to achieve the effects of improving comprehensive utilization, simple production process, and increasing added value

Inactive Publication Date: 2013-07-24
宜昌湘宜水产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method for preparing collagen powder through fish scales, which can solve the problem of utilizati...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Deodorization and decalcification of fish scales

[0028] Put the fish scales collected in the process of silver carp processing into the container, by weight, add tap water to the fish scales by fish scales: water=1:1, then add the Bacillus subtilis bacterium liquid of 3% of the above-mentioned material gross weight, at 35 ℃, 180r / min shaking culture for 2 hours for deodorization treatment, then add 2% by weight of HCl solution with a mass concentration of 5% to the obtained material, and shake and react at 150r / min for 10 minutes for decalcification treatment; The fish scales are washed repeatedly with clean water to remove the silt, bacteria and impurities, and cleaned for later use;

[0029] Wherein the Bacillus subtilis bacteria liquid is obtained by expanding the slant strain of the Bacillus subtilis in a shake flask, and the culture conditions are: 39° C., 120 r / min shaking culture for 24 hours.

[0030] 2) Frozen crushing treatment

[0031] Freeze the washe...

Embodiment 2

[0040] 1) Deodorization and decalcification of fish scales

[0041] Put the fish scales collected during grass carp processing into a container, and add tap water to the fish scales according to fish scales: water = 1:1 by weight, and then add 5% of the total weight of the above-mentioned materials. , 120r / min shaking and culturing for 1 hour for deodorization treatment, then adding 3% by weight of HCl solution with a mass concentration of 5% to the obtained material, and carrying out decalcification treatment at 180r / min shaking reaction for 12 minutes; fish scales after treatment Rinse with clean water repeatedly to remove the sediment, bacteria and impurities, and clean it for later use;

[0042] Wherein, the Bacillus subtilis bacteria liquid is obtained by expanding the culture of the slant strain of Bacillus subtilis in a shake flask, and the culture conditions are: 35°C, 150r / min shaking culture for 30 hours;

[0043] 2) Frozen crushing treatment

[0044] Freeze the wa...

Embodiment 3

[0053] 1) Deodorization and decalcification of fish scales

[0054] Put the fish scales collected during the herring processing into a container, add tap water to the fish scales according to fish scales: water = 1:1 by weight, and then add 4% of the total weight of the above-mentioned materials. , 150r / min shaking and culturing for 1.5 hours for deodorization treatment, then adding 2% by weight of HCl solution with a mass concentration of 5% to the obtained material, and performing decalcification treatment at 150r / min shaking reaction for 10 minutes; the treated fish scales Rinse with clean water repeatedly to remove the sediment, bacteria and impurities, and clean it for later use;

[0055] Wherein the Bacillus subtilis bacteria liquid is obtained by expanding the slant strain of Bacillus subtilis in a shake flask, and the culture conditions are: 35°C, 150r / min shaking culture for 36 hours;

[0056] 2) Frozen crushing treatment

[0057] Freeze the washed fish scales at -7...

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PUM

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Abstract

The invention discloses a method for preparing collagen protein powder from fish scales. The method comprises the following steps of: (1) deordorization, decalcification and washing treatment on the fish scales; (2) cryogenic grinding treatment on the fish scales; (3) steam explosion treatment on the fish scales; (4) enzyme hydrolysis of the fish scales; and (5) preparation of the collagen protein powder from the fish scales. The production method provided by the invention is simple, less in dosage of protease, low in production cost, short in processing cycle, mild in condition, high in utilization rate of equipment and easy to realize industrialized production, and can reach an average extraction rate of above 70%. The collagen protein content in the prepared collagen protein powder reaches above 95%, the in vitro digestibility of the powder reaches above 95%, and the problem of utilization of fish scales in fish processing is well solved, so that the additional value of leftovers in the processing of aquatic products increases, and the comprehensive utilization rate of freshwater fish is improved.

Description

technical field [0001] The invention relates to a preparation method of collagen, more specifically, relates to a method for preparing and extracting collagen powder using fish scales as raw materials. Background technique [0002] my country is a large fishery country. In recent years, with the increase of the total amount of aquaculture aquatic products and processing demand in our country, the pressure of aquatic product processing waste on the environment has become increasingly significant. Getting stronger and stronger. During the production and processing of aquatic products, fish scales, fish skins, viscera and other offals will be produced. Fish scales account for 3-5% of this part of the offals and are rich in collagen. Since the 1980s and 1990s, fish scales have been used more and more extensively as biomaterials for the repair of human organs and the manufacture of artificial organs. Contents of the invention [0003] The technical problem to be solved by the ...

Claims

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Application Information

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IPC IPC(8): A23J1/04
Inventor 李作卫
Owner 宜昌湘宜水产品有限公司
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