Nitrogen supply recovery specific induced expression promoter Y2 after paddy rice nitrogen deficiency, and application thereof

A technology for inducing expression and promoters, which is applied in the field of plant genetic engineering and can solve the problems of single plant biological yield and grain yield decline
CN103255140AInactive Publication Date: 2013-08-21HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2013-08-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of plant genetic engineering, and specifically relates to a nitrogen supply recovery specific induced expression promoter Y2 after paddy rice nitrogen deficiency, and an application thereof. With a chip technology, promoter Y2 downstream gene nitrogen supply induced expression information is obtained. With different paddy rice varieties, Y2 downstream gene nitrogen supply induced expression mode is verified. With a PCR method, the promoter Y2 full length and a 5'-terminal truncated fragment thereof are amplified, and are connected to a promoter vector DX2181b, such that a promoter fusion GUS expression vector is obtained. The constructed vector is used in genetic transformation upon a paddy rice variety Zhonghua11. The regulation of the promoter upon GUS gene is verified in a transformation positive plant. Through Realtime expression level verification and GUS protein activity detection, it is further determined that the promoter is a nitrogen supply recovery specific induced expression promoter after nitrogen deficiency. A functional section is at AT6 upstream 519bp.
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Description

technical field

[0001] The invention relates to the technical field of plant genetic engineering. In particular, it relates to a promoter Y2 that restores nitrogen supply specific induction expression after nitrogen deficiency in rice and its application Background technique

[0002] At present, transgenic research mainly uses constitutive promoters to drive the expression of target genes, the most typical is the CaMV35S promoter isolated from cauliflower mosaic virus (Hirt et al., 1990; Battraw et al., 1990), and some plant sources in recent years promoters, such as the promoter of actin gene Actin1 cloned from rice and the ubiquitin gene promoter cloned from maize (Schledzewski et al., 1994). However, the constitutive expression of exogenous genes will often cause unnecessary waste of resources, and the accumulation of a large number of heterologous proteins will also break the original metabolic balance of plants and hinder the normal growth of plants (Nie Lina et al., 2...

Claims

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