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Livestock seminal fluid cryoprotectant and application thereof

A technology of cryoprotectant and semen, which is applied in application, animal husbandry, preservation of human or animal bodies, etc., can solve problems such as difficulty in popularization and application, low conception rate of female livestock, easy damage, etc., and achieves favorable promotion and application and good market value and social value effects

Active Publication Date: 2013-08-28
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although livestock semen freezing technology is of great significance in livestock breeding and germplasm resource preservation, it is difficult to popularize and apply the livestock semen cryopreservation technology at present, mainly because livestock sperm itself is particularly sensitive to temperature changes, and it is extremely difficult to Easy to damage, which makes the production of frozen semen of livestock more difficult, and the recovery rate of sperm after thawing is low, resulting in low conception rate and small litter size of female animals
This technical problem seriously restricts the improvement and breeding process of livestock breeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Step 1 to prepare liquid A:

[0032] 50g glucose, 1.5g EDTA, 4g sodium citrate dihydrate, 1g sodium bicarbonate, 0.1g potassium chloride, 1×10 6 IU penicillin, 1×10 6 IU streptomycin, dilute to 1L with distilled water, stir and mix to obtain liquid A. After filtration and disinfection with a 0.22 μM microfiltration membrane, store at 5°C for use.

[0033] Step 2 Prepare B solution

[0034] 21.9g fructose, 18.25g tris, 10.95g citric acid, 1×10 6 IU penicillin, 1×10 6 IU streptomycin, 2% glycerin, 2% DMF, 2% ethylene glycol, 20% egg yolk, dilute to 1L with distilled water, stir and mix to obtain liquid B. After filtration and disinfection with a 0.22 μM microfiltration membrane, store at 5°C for use.

[0035] Step 3 Freezing semen:

[0036] Large gram boars with high fecundity were selected, and semen was collected by hand holding. Collect the sperm-rich part, measure the semen density and motility immediately, collect about 50 mL of semen with a motility rate of ...

Embodiment 2

[0042] According to the steps of Example 1, semen cryoprotectants with different main components were prepared for comparative tests. The final concentrations of glycerol, DMF and ethylene glycol in the test B liquids were shown in Table 1, and the other components were the same.

[0043] According to the method of Example 1, different configurations of semen cryoprotectants were used in the pig semen cryopreservation test, and each configuration was repeated three times. The results are shown in Table 2.

[0044] Table 1 Main components in liquid B of different experimental groups

[0045] group

[0046] Table 2 Effects of different mixing ratios on the quality of pig frozen sperm

[0047] group

[0048] After the significance test, the survival rate of experimental group 9 after thawing was extremely significant (p<0.01) compared with experimental group 1-8, and it was significant (p<0.05) compared with experimental group 10. The survival rate of experime...

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Abstract

The invention provides a livestock seminal fluid cryoprotectant and an application thereof. The cryoprotectant comprises a fluid A and a fluid B, wherein the fluid A includes glucose, edetic acid, dihydrate sodium citrate, sodium bicarbonate, potassium chloride, penicillin and streptomycin, and the fluid B includes fructose, tris hydroxymethyl aminomethane, citric acid, penicillin, streptomycin, glycerol, DMF (Dimethyl Formamide), glycol and yolk. According to the livestock seminal fluid cryoprotectant, frequently-used three refrigerants of glycerol, glycol and DMF are optimized and combined, so that an optimum compatibility effect of the three cryoprotectants is achieved. The selected cryoprotectant composition capable of improving a cryopreservation effect of the livestock seminal fluid can effectively improve the cryopreservation survival efficiency of the livestock seminal fluid, can maintain the integrity of the acrosome, can be widely popularized and applied to the field of livestock cultivation, and has favorable market effects and social effects.

Description

technical field [0001] The invention relates to the technical field of improving artificial insemination of livestock, in particular to a cryoprotectant capable of improving the quality of frozen semen of livestock and a preparation method thereof. Background technique [0002] Semen cryopreservation refers to placing the semen in liquid nitrogen (-196°C) or dry ice (-79°C) after special treatment, so that the metabolic activity of the sperm is temporarily stopped, so that the germplasm resources of the species can be preserved for a long time. The purpose of effectively utilizing the genetic resources of fine-bred male animals. This technology can greatly improve the utilization rate of high-quality male animals, reduce the amount of breeding, save costs, and directly help farmers and herdsmen to increase production and income. At the same time, it can also alleviate the current domestic human and livestock competition for food, reduce greenhouse gas emissions, and reduce ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 许晓玲刘彦白佳桦冯涛黄正
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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