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Method for separating microalgae through in-situ flocculation and application thereof

An in-situ flocculation and microalgae technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of the influence of medium circulation and the high cost of microbial flocculants, so as to achieve low cost, prevent loss, The effect of low energy consumption

Inactive Publication Date: 2013-08-28
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of microbial flocculants is high, and the recycling of medium is also affected by residual microbial flocculants

Method used

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  • Method for separating microalgae through in-situ flocculation and application thereof
  • Method for separating microalgae through in-situ flocculation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the separation of Chlorococcus ellipsoides

[0042] (1) Preparation of BG-11 culture medium: NaNO 3 (1.5g / L); K 2 HPO 4 ·3H 2 O (40mg / L); MgSO 4 ·7H 2 O (75mg / L); CaCl 2 2H 2 O (36 mg / L); Na 2 CO 3 (20mg / L); FeCl 3 ·6H 2 O (3.15mg / L); citric acid (6mg / L) and 1ml of trace element solution including: H 3 BO 3 (2.86mg / L), MnCl 2 4H 2 O (1.18mg / L), ZnSO 4 ·7H 2 O (0.22 mg / L), Na 2 MoO 4 2H 2 O (0.39mg / L), CuSO 4 ·5H 2 O (0.08mg / L), Co (NO 3 ) 2 ·6H 2 O (0.05mg / L), add until 1ml concentrated H 2 SO 4 (concentration 98% (v / v)) in 1L of distilled water, and stir evenly to obtain BG-11 culture medium.

[0043] (2) The BG-11 culture solution prepared in step (1) was used to cultivate Chlorococcum ellipsoideum UTEX972 in the incubator (purchased from the algae collection center of the University of Texas at Austin, USA). Take 2L of BG-11 culture solution for cultivation, and adjust the implantation amount of microalgae to OD 750 ≈0.6, at ...

Embodiment 2

[0048] Embodiment 2: Isolation of Pideurophylla

[0049] (1) The preparation of BG-11 culture medium is the same as that in Example 1.

[0050] (2) The BG-11 culture solution prepared in step (1) was used to cultivate Chlorococcum nivale UTEX LB2225 in the incubator (purchased from the algae collection center of the University of Texas at Austin, USA). Measure 2L of BG-11 culture solution for cultivation, the amount of microalgae implantation, cultivation conditions and cultivation time are the same as in Example 1.

[0051] When the dry weight of Pideurophylla in the culture solution is greater than 1.0g / L, use 1mol / L HNO 3 Adjust the pH value of the culture solution to 4.0, stir for 1 min at a rotation speed of 280 rpm, let stand to separate layers, and pour out the supernatant to obtain Pidelodioides. The calculation method of separation rate is the same as embodiment 1. Calculated by the formula, the separation rate after standing for 30 minutes was 90.5%.

[0052] (3)...

Embodiment 3

[0053] Embodiment 3: Isolation of Bacteroides chlorella

[0054] (1) The preparation of BG-11 culture medium is the same as in Example 1;

[0055] (2) Bracteacoccus minor UTEX B66 (Bracteacoccus minor UTEX B66, purchased from the University of Texas at Austin Algae Collection Center) was cultivated in the incubator using the BG-11 culture medium prepared in step (1). Measure 2L of BG-11 culture solution for cultivation, the amount of microalgae implantation, cultivation conditions and cultivation time are the same as in Example 1. When the dry weight of Bacteroides spp. in the culture medium is greater than 1.0g / L, use 1mol / L of HNO 3 Adjust the pH value of the culture solution to 4.3, stir for 1 min at a rotational speed of 300 rpm, let stand to separate layers, and pour out the supernatant to obtain Bacteria chlorella. (The calculation method of the separation efficiency is the same as in Example 1) The separation rate after standing for 30 minutes is calculated by the for...

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Abstract

The invention belongs to the field of biomass energy, and in particular relates to a method for separating microalgae through in-situ flocculation and application thereof. The method comprises the following steps of: adding acid liquor into a culture solution for culturing the microalgae, regulating the pH value to be acidic, flocculating and settling the microalgae, standing, demixing, and separating the supernate to obtain separated culture solution and microalgae. The separated microalgae culture solution is recycled for culturing the microalgae after alkali liquor neutralization and regulation. The method has the advantages of simplicity in operation, wide reagent source, low cost, high efficiency and low energy consumption, and the settling separation rate within 30 minutes reaches more than 90 percent.

Description

technical field [0001] The invention belongs to the field of biomass energy, in particular to a method for in-situ flocculation and separation of microalgae and its application. Background technique [0002] Microalgae (Microalga) are single-celled algae with small size, simple structure, and rapid growth, which are widely distributed. At the same time, it is also an autotrophic and oxygen-releasing plant with rich nutrition and strong photosynthesis, and the annual fixed CO 2 Accounting for about 40% of global net photosynthesis, it plays a pivotal role in energy conversion and carbon cycle. [0003] Microalgae is an economical and efficient biomass energy. The rich oil (20-80%) can be used as raw material to obtain biodiesel. Compared with other bioenergy sources (such as corn, cassava, etc.), microalgae has different Competing for land, not competing with others for food, fast growth rate, short growth cycle, high oil content and many other advantages occupy an importan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 张渊明朱毅刘洁霞陶玉君张成武李爱芬李涛
Owner JINAN UNIVERSITY
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