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Dendritic molecule-modified fluorescent quantum dots, and preparation method and application thereof

A fluorescent quantum dot and dendritic technology, applied in the field of biomedical engineering materials, can solve the problems of functional development and utilization of quantum dots (environmental response bottleneck, lack of modifiable groups, lack of stability of quantum dots, etc., to improve stability) Sexuality and biocompatibility, easy operation, and low toxicity of metabolites

Inactive Publication Date: 2013-09-04
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method adds too much metal and inorganic substances in the preparation process of water-soluble fluorescent quantum dots, which not only increases the difficulty of material regulation, but also increases the risk of quantum dots in the body; moreover, the obtained quantum dots lack long-term Stability over time and the lack of modifiable groups create a bottleneck for the functional development and utilization of quantum dots (environmental response, targeting, etc.)

Method used

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  • Dendritic molecule-modified fluorescent quantum dots, and preparation method and application thereof
  • Dendritic molecule-modified fluorescent quantum dots, and preparation method and application thereof
  • Dendritic molecule-modified fluorescent quantum dots, and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0055] Example 1: Preparation of Dendrimer Modified Fluorescent Quantum Dots

[0056] (1) Synthesis of dendrimers whose end groups are siloxanes:

[0057] Weigh the second-generation polyamide-amine dendrimers (PAMAM-G2) containing terminal alkyne groups (synthesized according to the reference method (Journal of Materials Chemistry2011, 21:5273-5281)) and 3-mercaptopropyl trimethyl The base siloxane was dissolved in DMSO, and then the BPO initiator was added at 5°C. Nitrogen was passed through the mixed solution for 10-30 minutes to remove the air in the reaction system, and the gas flow rate was 5 L / min. Then sealed and reacted, magnetic stirring during the reaction, the rotating speed was 600 rpm; the reaction temperature was 90° C.; the reaction time was 4 hours. After the reaction is finished, the product is precipitated with anhydrous ether, washed once with acetone, and then vacuum-dried at room temperature to obtain polyamide-amine dendrimers whose end groups are silo...

Embodiment 2

[0062] Example 2: Preparation of fluorescent quantum dots modified by dendrimers

[0063] (1) Synthesis of dendrimers whose end groups are siloxanes:

[0064] Weigh the three-generation polyamide-amine dendrimer (PAMAM-G3) containing terminal alkyne group (synthesized according to the reference method (Journal of Materials Chemistry2011, 21:5273-5281)) and 3-mercaptopropyltrimethyl Siloxane was dissolved in DMSO, and AIBN initiator was added at 25°C. Nitrogen was passed through the mixed solution for 10-30 minutes to remove the air in the reaction system, and the gas flow rate was 0.5 L / min. Then it was sealed and reacted. During the reaction, it was stirred by magnetic force at a rotation speed of 1200 rpm; the reaction temperature was 40° C.; the reaction time was 12 hours. After the reaction finishes, the product is precipitated with anhydrous ether, and washed once with acetone, then the product is vacuum-dried at room temperature to obtain a polyamide-amine dendritic mo...

Embodiment 3

[0070] Example 3: Preparation of Dendrimer Modified Fluorescent Quantum Dots

[0071] (1) Synthesis of dendrimers whose end groups are siloxanes:

[0072] Weigh the four-generation polyamide-amine dendrimer (PAMAM-G4) containing terminal alkyne group (synthesized according to the reference method (Journal of Materials Chemistry2011, 21:5273-5281)) and 3-mercaptopropyltrimethyl The base siloxane was dissolved in DMSO, and then the MK initiator was added at 35°C. Nitrogen was passed through the mixed solution for 10-30 minutes to remove the air in the reaction system, and the gas flow rate was 2.5 L / min. Then sealed and reacted, the reaction system was placed under an ultraviolet lamp with an excitation wavelength of 365nm, the power was 400W, magnetic stirring was performed during the reaction, the rotation speed was 1000rpm, and the reaction time was 6h. After the reaction is finished, the product is precipitated with anhydrous ether, washed once with acetone, and then vacuu...

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Abstract

The invention belongs to the field of biomedical engineering materials, and particularly relates to dendritic molecule-modified fluorescent quantum dots, and a preparation method and an application thereof. The preparation method comprises the following steps of (1) dissolving the dendritic molecule and 3-mercaptopropyl trimethyl siloxane in dimethyl sulfoxide, adding an initiator, introducing the gas to expel oxygen, carrying out a sealed reaction, precipitating, filtering, washing, and drying to obtain the dendritic molecule with siloxane terminals; and (2) dissolving the dendritic molecule with the siloxane terminals obtained by the step (1) and water-soluble quantum dots in water, loading the solution in a dialysis bag, putting the dialysis bag in a solution, immersing the dialysis bag with stirring, then immersing in water, and freeze-drying to obtain the dendritic molecule-modified fluorescent quantum dots. The preparation method is mild, convenient for operations and little in by-products. Besides, the dendritic molecule-modified fluorescent quantum dots can be separated and purified easily, and have good biocompatibility. A great amount of modifiable functional groups on the surfaces of the quantum dots can provide supports for the application of the quantum dots in preparing the biomedical engineering materials.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials, and in particular relates to a fluorescent quantum dot modified by a dendritic molecule and a preparation method and application thereof. Background technique [0002] Due to their special physical and chemical properties, quantum dots (CdS, CdTe, etc.) have shown broad application prospects in the fields of photoelectric conversion, medical imaging, and bioluminescent probes. Compared with organic small molecules commonly used for fluorescent labeling, quantum dots have the advantages of narrow particle size distribution, wide and continuous absorption spectrum, narrow and symmetrical emission spectrum, and high luminous intensity, and fluorescent quantum dots can pass through particle size To adjust its emission wavelength, can effectively avoid the photobleaching effect. [0003] Aldana et al. used mercaptopropionic acid to modify CdSe quantum dots to obtain fluorescent quantum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/56C09K11/88C09K11/02C12N15/87
Inventor 马栋薛巍周小雁郑倩倩刘宗华左琴华
Owner JINAN UNIVERSITY
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