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Alginate-chitosan microcapsule modified via PEG grafting and preparation and application thereof

A technology of chitosan microcapsules and alginate, which is applied in the direction of microcapsule preparation, microsphere preparation, immobilization on/in organic carriers, etc., and can solve the problems of large surface roughness and surface charge, protein adsorption, etc. , to achieve the effects of improving stability, increasing graft density, and excellent biocompatibility

Active Publication Date: 2013-09-18
ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing chitosan microcapsules used for cell embedding have a large surface roughness and surface charge, which can easily cause protein adsorption after transplantation in vivo, further stimulating the body's fibrosis reaction

Method used

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  • Alginate-chitosan microcapsule modified via PEG grafting and preparation and application thereof
  • Alginate-chitosan microcapsule modified via PEG grafting and preparation and application thereof
  • Alginate-chitosan microcapsule modified via PEG grafting and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Terminal aldylation of PEG: The molecular weight of PEG is 500 Da. The terminal hydroxyl group of PEG is oxidized into aldehyde group by oxidation method to prepare PEG-CHO. The PEG-CHO is dissolved in normal saline at a concentration of 10 g / L.

[0038] 2) Preparation of chitosan solution: chitosan molecular weight 20kDa, deacetylation degree 90%, dissolved in sodium acetate / acetic acid buffer solution with pH 6.5, concentration 10g / L.

[0039] 3) Preparation of AC microcapsules: immerse calcium alginate gel beads prepared by high-voltage electrostatic method into chitosan solution, the volume ratio of microspheres and chitosan solution is 1:40, react for 20 minutes, wash with normal saline, and prepare AC microcapsules.

[0040] 4) Immerse the AC microcapsules in the PEG-CHO solution, drop the reducing agent sodium triacetoxyborohydride solution, the concentration of sodium triacetoxyborohydride is 0.1g / L, the microspheres, PEG-CHO and triacetoxy The volume ratio ...

Embodiment 2

[0057] 1) Terminal aldylation of PEG: The molecular weight of PEG is 2 kDa, and the hydroxyl group at the end of PEG is oxidized to form an aldehyde group by an oxidation method to prepare PEG-CHO. The PEG-CHO is dissolved in normal saline at a concentration of 5 g / L.

[0058] 2) Preparation of chitosan solution: chitosan molecular weight 60kDa, deacetylation degree 90%, dissolved in sodium acetate / acetic acid buffer solution of pH 6.5, concentration 5g / L, the solution is represented by C.

[0059] 3) Immerse the calcium alginate gel microspheres embedded with porcine islet cells in the chitosan solution prepared in step 2), the volume ratio of the microspheres to the chitosan solution is 1:10, react for 20 minutes, and wash with normal saline , prepared into AC microcapsules.

[0060] 4) Immerse the AC microcapsules prepared in step 3) into the PEG-CHO solution prepared in step 1), add dropwise the reducing agent sodium cyanoborohydride solution, the concentration of sodium c...

Embodiment 3

[0064] 1) Terminal aldylation of PEG: The molecular weight of PEG is 10kDa, and the hydroxyl group at the end of PEG is oxidized into aldehyde group by oxidation method to prepare PEG-CHO, which is dissolved in normal saline at a concentration of 5g / L.

[0065] 2) Preparation of chitosan solution: chitosan molecular weight 100kDa, deacetylation degree 90%, dissolved in sodium acetate / acetic acid buffer solution of pH 6.5, concentration 5g / L, solution is represented by C.

[0066] 3) Immerse the calcium alginate gel microspheres embedded with pig liver cells in the chitosan solution prepared in step 2), the volume ratio of the microspheres to the chitosan solution is 1:10, react for 10 minutes, and wash with normal saline , prepared into AC microcapsules.

[0067] 4) Immerse the AC microcapsules prepared in step 3) into a 1.5 g / L sodium alginate solution, react for 10 minutes, wash with physiological saline, and prepare ACA microcapsules.

[0068] 5) Repeat step 3) to prepare ...

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Abstract

The invention relates to an alginate-chitosan microcapsule modified via in situ covalent PEG grafting. The structure of the microcapsule is divided into two parts, i.e., a microcapsule membrane and an inner core, wherein the microcapsule membrane is a polyelectrolyte composite hydrogel membrane formed by chitosan and alginate, the surface of the membrane is modified via in situ covalent PEG grafting, and the inner core is an alginate liquid or alginate hydrogel environment containing living cells. Because of resistance to protein adsorption, the microcapsule is applied to living cell encapsulation and used as an immunoisolation carrier for cell transplantation.

Description

technical field [0001] The present invention relates to a kind of alginate microcapsule product that is used for live cell embedding, specifically the preparation of a kind of alginate-chitosan microcapsule modified by in-situ covalent grafting of PEG and its application in living cells. Applications in cell embedding. Background technique [0002] In the 1960s, Chang reported semipermeable membrane microcapsules, pointing out that bioactive substances and cells such as proteins and enzymes could be embedded in them, which could maintain the activity of biological substances [Chang TMS.Semipermeable microcapsules, Science, 1964, 146: 524-525 ]. In the early 1980s, Lim and Sun successfully prepared sodium alginate / α-polylysine (alginate / α-polylysine) semipermeable membrane microcapsules (referred to as α -APA microcapsules), encapsulated Wistar rat islet cells and transplanted into diabetic WistarLewis rats, secreted and released insulin to regulate blood sugar [Lim F, Sun ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02C12N11/04C12N11/10C08G81/00C08B37/08C08B37/04
Inventor 马小军郑佳妮于炜婷谢红国刘袖洞谢威扬
Owner ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
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