Method for producing cordyceps vinegar by use of cordyceps taishanensis fermentation liquor
A technology of Cordyceps fungus and fermentation liquid, applied in the food field, can solve the problems of unbalanced product quality, long cycle, low solid culture production efficiency, etc., and achieve the effect of shortening fermentation cycle, stable product quality, and full utilization of resources.
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Embodiment 1
[0013] Example 1 A method for producing cordyceps vinegar from the fermentation broth of Cordyceps fungus: (1) Activate the cordyceps fungus with a wort medium, and after aseptic inoculation, shake the flask with a flask shaker at 25°C, 150r / min (revolutions per minute) Machine shaking culture for 3 days to prepare Cordyceps seed liquid. (2) Crush the barley malt used in beer production until the powder passes through a 20-mesh sieve, while the bark is broken but not broken, add 4 times water, carry out saccharification reaction at 65°C for 1.5 hours, and then filter to make 10°P concentration barley juice , Mixed with the same volume of sucrose solution of the same concentration; add 5% acacia flowers; sterilize at 121°C for 15 minutes. (3) Aseptic operation, inoculate the cordyceps fungus seed liquid at 5% into the wort, sucrose and acacia flower mixed solution medium, and pass in sterile air 0.02m 3 / m 3 / h, cultured at 24°C for 5 days, and then centrifuged to filter out Cor...
Embodiment 2
[0014] Example 2 A method for producing cordyceps vinegar by using the fermentation broth of Cordyceps fungus: (1) Activate the cordyceps fungus with a wort medium, and after aseptic inoculation, culture in a flask shaker at 25°C and 150r / min for 3 days to prepare cordyceps Seed liquid. (2) Crush the beer malt until the powder passes through a 20-mesh sieve. The bark is broken but not broken. Add 4 times water and carry out the saccharification reaction at 60°C for 3 hours, and then filter to make 12°P barley juice with the same concentration. Mix the sucrose solution with equal volume; add 5% acacia flowers; sterilize at 121°C for 15 minutes. (3) Aseptic operation: Inoculate the cordyceps fungus seed liquid at 10% into the mixed solution medium of wort, sucrose and acacia flower, and pass in sterile air 0.02m 3 / m 3 / h, culture at 26°C for 7 days, and then centrifuge to remove Cordyceps mycelium. (4) Glucose 1%, yeast extract 1%, calcium carbonate 1%, absolute ethanol 3%, pH ...
Embodiment 3
[0015] Example 3 A method for producing cordyceps vinegar by using the fermentation broth of Cordyceps fungus: (1) Activate the cordyceps fungus with a wort culture medium, and after aseptic inoculation, culture in a flask shaker at 25°C, 150r / min for 3 days to prepare cordyceps Seed liquid. (2) Crush the beer malt until the powder passes through a 20-mesh sieve, the bark is broken but not broken, add 5 times water, 68°C for saccharification for 1 hour, filter to make 8°P barley juice, and the same concentration of sucrose solution Mix equal volume; add 1% acacia flower; sterilize at 121°C for 15 minutes. (3) Aseptic operation, inoculate the cordyceps fungus seed liquid at 8% into the mixed solution medium of wort, sucrose and acacia flower, culture in a shake flask at 22°C for 7 days, and then centrifuge to remove the cordyceps mycelium. (4) Glucose 1%, yeast extract 1%, calcium carbonate 1%, absolute ethanol 3%, pH natural, make acetic acid bacteria liquid medium, sterilize ...
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