Acetobacter pasteurianus and application thereof

A technology of Acetobacter pasteuriani and seeds, applied in Acetobacter pasteuriani Ab3 and its application fields, can solve the problems that the production of dihydroxyceramide has not been reported, and achieve the effect of high yield

Active Publication Date: 2013-09-18
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the production of dihydro

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Acetobacter pasteurian Ab3 can grow at 28-35°C under acidic pH conditions. Acetobacter pasteurian Ab3 belongs to the genus Acetomonas, and the cells range from oval to rod-shaped, solitary, in pairs or in chains. In old cultures, it is prone to various deformities, such as spherical, filamentous, rod-shaped, curved, etc. Acetic acid bacteria have two types of flagella: one group is the periphytic flagellar bacteria, which can further oxidize acetic acid to carbon dioxide and water; the other group is the polar flagellar bacteria, which cannot further oxidize acetate. Both groups are Gram-negative bacilli. Acetobacter pasteurianus Ab3 belongs to the genus Acetobacter, with >99% homology with Acetobacter pasteurianus.

Embodiment 2

[0025] The Acetobacter pasteurianum screened from rose balsamic vinegar was cultivated by spreading it on the nutrient medium, and then the colony with a large transparent circle was picked out and inoculated on the nutrient medium for cultivation. The nutrient medium contained 6Bx o Wort, 0.04% KH 2 PO 4 , 0.02% MgSO 4 , 0.05% CaCl 2 , 4% ethanol, 2% agar, the pH of the nutrient medium is 4.5, the culture temperature is 30°C, and the culture time is 3 days. Take the slant bacteria, add 5ml of physiological saline (per 18*180mm test tube slant bacteria), gently wash off the bacteria lawn with an inoculation stick to make a cell suspension. Using the hemocytometer method, adjust the bacterial concentration in the bacterial suspension to 108 cells / mL. The freshly prepared bacterial suspension was taken, centrifuged and precipitated, resuspended in an appropriate amount of sterile water, divided into 3 tubes, and irradiated with 60Co γ-rays at the irradiation doses of 300 Gy,...

Embodiment 3

[0027] Inoculate 5Bx with Acetobacter pasteurii Ab3 o Wort, 0.02% KH 2 PO 4 , 0.01% MgSO 4 , 0.05% CaCl 2 , ethanol 3%, agar 1.5%, nutrient medium pH=6.5, culture temperature 28°C, culture time 2 days. Take the slant bacteria, add 5ml of physiological saline (per 18*180mm test tube slant bacteria), gently wash off the bacteria lawn with an inoculation stick to make a cell suspension. Take 10ml and inoculate it into 90ml seed medium for 1 day, the seed medium contains 2% glucose, 0.5% yeast extract, 0.1% peptone, 0.08% KH 2 PO 4 , 0.01% MgSO 4 , 5% ethanol, the balance of water, the pH of the seed medium is 5.0, the culture temperature is 30°C, and the rotation speed is 120 rpm. Then 20ml of seed liquid was inserted into a 500ml shake flask with a liquid volume of 80ml of fermentation medium, the temperature was 32°C, and the rotation speed was 220 rpm, and cultured for 2 days to obtain Acetobacter pasteurian Ab3 fermentation liquid, and the bacteria were collected by ce...

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Abstract

The invention provides an acetobacter pasteurianus and application thereof, and belongs to the technical field of bioengineering. The acetobacter pasteurianus (Acetobacterpasteurianus) Ab3 is stored with a number of CCTCC (China General Microbiological Culture Collection Center) No: M2013116. The invention also discloses application of the acetobacter pasteurianus Ab3 in fermentation production of C18 dihydroceramide and application in fermentation production of table vinegar. The acetobacter pasteurianus is high in yield of the C18 dihydroceramide obtained in fermentation broth per unit volume; the result of quantitative analysis by HPLC (High Performance Liquid Chromatography) shows that the yield of the C18 dihydroceramide in the fermentation broth per unit volume is up to 8.69mg/L; and the yield of the C18 dihydroceramide obtained by the supercritical CO2 extraction technique and the high-speed counter-current chromatography is up to 83.7%, and the purity reaches 92.5%.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to Acetobacter pasteurianum Ab3 and an application thereof. Background technique [0002] The physiological function and application fields of ceramide are described. Ceramides are important components of the stratum corneum of the skin. At the same time, as second messengers in cells, they participate in intracellular signal transduction by activating various protein kinases and transcription factors, thereby regulating cell growth, proliferation, differentiation, apoptosis and damage. and other physiological and pathological processes. In recent years, relevant studies have found that ceramides play the role of active factors in skin care, and have important functions such as skin moisturizing, protection, and repair of damage. Therefore it has become an important cosmetic additive component. Ceramide is an important functional substance in the living body. I...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/02C12J1/00C12R1/02
CPCY02P20/54
Inventor 梁新乐施结燕
Owner ZHEJIANG GONGSHANG UNIVERSITY
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