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Handheld general microfluidic chip real-time detection device and application

A technology of microfluidic chips and detection devices, which is applied in enzymology/microbiology devices, biochemical cleaning devices, specific-purpose bioreactors/fermenters, etc. Expensive and other issues, to achieve the effect of good integration, low cost and easy operation

Active Publication Date: 2013-09-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]The traditional on-chip reaction control and detection device is bulky, expensive, high power consumption, requires external power supply, large computer
Higher operating requirements for users

Method used

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  • Handheld general microfluidic chip real-time detection device and application
  • Handheld general microfluidic chip real-time detection device and application
  • Handheld general microfluidic chip real-time detection device and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The temperature control system is composed of microprocessor 2, temperature sensor 8, heating plate 9, the temperature adjustment range is 0-100°C, the adjustment accuracy is 0.1°C, and the reaction temperature in the microchip 7 is precisely controlled.

[0029] The surface of the microchip 7 is kept smooth and transparent, and the manufacturing materials are selected from glass, silicon, polymethyl methacrylate, epoxy resin, polytetrafluoroethylene, polystyrene, polydimethylsiloxane and the like.

[0030] The microchip 7 is closely attached to the heat conducting plate 10, and the temperature control module composed of the microprocessor 2, the temperature sensor 8 and the heating plate 9 controls the reaction in the microchip 7. After the reaction is completed, the left excitation light source 6' and the right excitation light source 6 irradiate from the opposite sides of the microchip 7, and the detection optical path is composed of the optical filter 5, the lens 4, ...

Embodiment 2

[0038] This example is carried out using the device in Example 1, the chip is carried out using the chip of Example 4 of the application number CN103071548A, and the subsequent isothermal amplification reaction of digital nucleic acid is carried out.

[0039] Specific steps:

[0040] 1. The microchip 7 uses polydimethylsiloxane (PDMS) as a material, and a template with a microchannel structure made by multilayer soft lithography technology is used as a mold, and the gas-permeable PDMS is poured and solidified on the mold.

[0041]2. The completed microfluidic PDMS chip is placed in a container for vacuum degassing. After the PDMS chip is vacuumed and degassed, the air pressure in the gas-permeable polymer block drops. Stop vacuuming and degassing and place it at normal pressure When it is down, the channel and the reaction unit (17) form a pressure difference with the external environment, and the pressure difference drives the sample solution to fill the reaction unit 17 even...

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Abstract

The invention provides a handheld general microfluidic chip real-time detection device. The detection device is composed of a smart mobile device, a microprocessor, a mobile power supply, a lens, a light filter, two excitation light sources, a microchip, a temperature sensor, a heating plate, a heat transfer plate, a wireless communication module, an built-in imaging chip of the smart mobile device, a built-in lens of the smart mobile device, a large server or a personal computer. The detection device has the advantages of simple and reasonable design structure, small size and low cost, and many defects of a traditional on-chip reaction control and detection device are overcome. The integrated design with low power consumption is combined with the self processing capacity and data transfer capability of the smart mobile device, an external power supply and an external large computer are not needed, and the applicable range of the detection device is greatly extended. Based on applications (APP) which operate on the smart mobile device, the detection device is easy to operate and can be applied to the nucleic acid amplification reaction.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chip reaction control and detection, and relates to a handheld general-purpose microfluidic chip real-time detection device. Background technique [0002] Due to the advantages of high integration and low reagent loss, microchips are widely used in many fields such as medical diagnosis, environmental protection, and drug screening. The application of microchips needs to cooperate with on-chip reaction control and reaction result detection. The basic method is: to precisely control the on-chip reaction through a high-precision temperature control module. After the reaction is completed, the excitation light source is irradiated into the microchannel or microreaction chamber. The fluorescent substance obtains energy and emits fluorescence, which is converted into an electrical signal by a photoelectric conversion device and processed by a computing device. [0003] The traditional on-chip reac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C12M1/38C12M1/34
Inventor 牟颖宋博凡金伟于丙文
Owner ZHEJIANG UNIV
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