The invention provides a handheld general microfluidic chip real-time detection device. The detection device is composed of a smart mobile device, a microprocessor, a mobile power supply, a lens, a light filter, two excitation light sources, a microchip, a temperature sensor, a heating plate, a heat transfer plate, a wireless communication module, an built-in imaging chip of the smart mobile device, a built-in lens of the smart mobile device, a large server or a personal computer. The detection device has the advantages of simple and reasonable design structure, small size and low cost, and many defects of a traditional on-chip reaction control and detection device are overcome. The integrated design with low power consumption is combined with the self processing capacity and data transfer capability of the smart mobile device, an external power supply and an external large computer are not needed, and the applicable range of the detection device is greatly extended. Based on applications (APP) which operate on the smart mobile device, the detection device is easy to operate and can be applied to the nucleic acid amplification reaction.