Bletilla lateral bud tissue culture propagation method

A technology of lateral buds and bletilla striata, applied in the field of tissue culture and propagation of lateral buds of bletilla striata, can solve the problems of low natural germination rate of seeds, failure to meet market needs, and scarcity of wild resources, etc., to shorten the cycle of tissue culture and reproduction, shorten production time, and inherit traits stable effect

Inactive Publication Date: 2013-09-25
重庆市秀山红星中药材开发有限公司
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The natural germination rate of Bletilla striata seeds is extremely low, reproduction is difficult, and wild resources are scarce. Due to the large demand, wild resources are already on the verge of depletion. They have been listed in the "Convention on International Trade in Endangered Species

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Prepare the medium first.

[0021] Germination medium: LS+6~BA1.2mg / L+NAA0.2mg / L+sucrose 30 g / L+agar 5g / L; pH5.8.

[0022] Induction medium: B001 BM+BA4+IAA1+carrageenan 5g / L+sucrose 30 g / L; PH6.0.

[0023] Proliferation medium: B002 BM+ IAA0.3+ carrageenan 5g / L+ sucrose 30 g / L; PH6.0.

[0024] Rooting and strong seedling medium: B003 BM+ IAA1+ carrageenan 5g / L+ sucrose 30 g / L; PH6.0.

[0025] Then select a strong wild bletilla striata, cut off the side buds, rinse under running water, soak in 1% detergent solution for 20 minutes, then gently scrub with a brush, and fully wash with sterile water; Place the lateral buds in 75% alcohol to disinfect for 30 seconds, and then use 0.2% HgCl 2 Soak in an aqueous solution for 7 minutes, then rinse with sterile water for 5 to 6 times, and then cut off the bottom of the lateral buds to form a new wound; inoculate the sterilized lateral buds as explants on the germination medium, and inoculate 5 lateral buds per bottle. Under ...

Embodiment 2

[0027] Select robust wild bletilla striata, cut off the side buds, rinse under running water, soak in 1% detergent solution for 20 minutes, then gently scrub with a brush, and fully wash with sterile water; put it on the ultra-clean workbench Disinfect the lateral buds in 75% alcohol for 30 seconds, and then use 0.2% HgCl 2 Soak in an aqueous solution for 8 minutes, then rinse with sterile water for 5 to 6 times, and then cut off the bottom of the lateral buds to form a new wound; inoculate the sterilized lateral buds as explants on the germination medium, and inoculate 6 lateral buds per bottle. Under the conditions of night temperature 23°C, day temperature 25°C, relative humidity 63%, light intensity 3000lx, daily light start time at 7:00 am, and daily light duration of 12 hours, the buds germinated and cultivated. After 15 days, the buds germinated. Within 30 days, 4-6 plants with leaves sprouted from each bottle, with a survival rate of 90%; the seedlings were cut off and...

Embodiment 3

[0029] Select robust wild bletilla striata, cut off the side buds, rinse under running water, soak in 1% detergent solution for 20 minutes, then gently scrub with a brush, and fully wash with sterile water; Disinfect the lateral buds in 75% alcohol for 30 seconds, and then use 0.2% HgCl 2Soak in aqueous solution for 9 minutes, then rinse with sterile water for 5 to 6 times, then cut off the bottom of the lateral buds to form new wounds; inoculate the sterilized lateral buds as explants on the germination medium, and inoculate 7 lateral buds per bottle. Under the conditions of night temperature 23°C, day temperature 25°C, relative humidity 65%, light intensity 3000lx, daily light start time at 7:00 am, and daily light duration of 12 hours, the buds germinated and cultivated. After 15 days, the buds germinated. ~30 days, 4~6 plants with leaves sprouted from each bottle, and the survival rate reached 90%; the seedlings were cut off and transferred to the induction medium, and 7 p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a bletilla lateral bud tissue culture propagation method, and belongs to the technical field of traditional Chinese medicine material plantation. The method comprises the following steps of introduction sterilization, bud sprouting culture, multiple shoot induction culture, multiplication culture and rooting culture. The lateral bud is adopted for tissue culture, so that the pollution rate of the obtained seedlings is low, the differentiation rate is high, inheritable character is stable, and the content of active substances is high; and meanwhile, the tissue culture propagation period of the bletilla is greatly shortened, the tissue culture period from the cutting of lateral bud to the rooting is less than 4 months, the production time is greatly shortened, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, in particular to a method for tissue culture and propagation of lateral buds of bletilla striata. Background technique [0002] Bletilla striata is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae, also known as white root, ground screw, white chicken baby, horn seven, purple orchid, knife-edge medicine, Lianjicao, etc. The tubers are used for medicine, which can restrain bleeding, reduce swelling and promote granulation; treat diseases such as tuberculosis and hemoptysis, bronchiectasis hemoptysis, gastric ulcer, hematemesis, hematuria and hematochezia. External use to treat traumatic bleeding, burns, chapped hands and feet embolism. It has huge commercial application value in biomedicine, health food, textile printing and dyeing, special coatings and daily chemical industry. With the development and utilization of bletilla stria...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 杨再江刘湘凤顾君龚成珍祝利
Owner 重庆市秀山红星中药材开发有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap