Edible fish skin collagen antibacterial film containing thyme essential oil
A fish skin collagen and essential oil technology, applied in animal/human protein, organic chemistry, flexible coverings, etc., to achieve good antibacterial activity, prolong fresh-keeping period, and prolong shelf life
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Embodiment 1
[0017] Weigh 1.2 g of collagen and dissolve it with 15 ml of distilled water in a stirring water bath at 45°C to make a collagen aqueous solution; homogeneously dissolve 0.6 ml of thyme essential oil in 15 ml of distilled water containing 0.075 g of soybean lecithin to make thyme essential oil solution, and then mix the two, add 0.3 g glycerin as a plasticizer, stir evenly, degas by ultrasonication for 30 min, and keep warm at 45 °C. Pipette 10 ml of casting solution each time and spread it on a plexiglass frame of 120 mm×80 mm×2 mm to form a film. Peel off the frame to obtain the collagen antibacterial film. The antibacterial activity of the membrane was measured by the filter paper method, and the membrane was cut into a circle of 12 mm to investigate the antibacterial effect of the antibacterial membrane on Escherichia coli and Staphylococcus aureus.
Embodiment 2
[0019] Weigh 1.2 g of collagen and dissolve it with 15 ml of distilled water in a stirring water bath at 45°C to make a collagen aqueous solution; homogeneously dissolve 1.2 ml of thyme essential oil in 15 ml of distilled water containing 0.075 g of soybean lecithin to make thyme essential oil solution, and then mix the two solutions, add 0.3 g glycerin as a plasticizer, stir evenly, degas by ultrasonication for 30 min, and keep warm at 45°C. Pipette 10 ml of casting solution each time and spread it on a plexiglass frame of 120 mm×80 mm×2 mm to form a film. Peel off the frame to obtain the collagen antibacterial film. The antibacterial activity of the membrane was measured by the filter paper method, and the membrane was cut into a circle of 12 mm to investigate the antibacterial effect of the antibacterial membrane on Escherichia coli and Staphylococcus aureus.
[0020] Table 1 The inhibition zones of the antibacterial films prepared under different conditions
[0021]
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