Method for detecting pear endogenous hormone by using ultra-high performance liquid chromatography electrospray tandem mass spectrum

Abstract

The invention discloses a method for detecting a pear endogenous hormone by using an ultra-high performance liquid chromatography electrospray tandem mass spectrum. The method comprises the following steps of: crushing obtained pear tissues, then adding an extracting solution for extraction, after ending extraction, adding an extraction reagent to a system, and extracting to obtain a crude extract of the endogenous hormone; separating and purifying the crude extract by using a solid-phase extraction column to obtain a detection sample; analyzing a detection sample by using the ultra-high performance liquid chromatography electrospray tandem mass spectrum to obtain the variety and the content of the endogenous hormone in the pear tissues, wherein the extracting solution comprises isopropanol, water and hydrochloric acid, and the volume ratio of the isopropanol, the water and the hydrochloric acid is (1.5-2.5):1:(0.001-0.002), and the extraction agent is dichloromethane. The method has the advantages of high sensitivity, simple pretreatment, high recovery rate, high degree of accuracy and degree of precision and the like and is used for detecting and confirming the endogenous hormones in multiple plant issues.

Description

technical field [0001] The invention relates to the technical field of spectrum analysis, in particular to a method for detecting pear endogenous hormones by using ultra-high performance liquid chromatography electrospray tandem mass spectrometry. Background technique [0002] Phytohormones are a type of organic substances produced by the plant's own metabolism, and move from the production site to the action site. They are trace substances that have obvious physiological effects at very low concentrations. They are also called plant natural hormones or plant endogenous hormones. . [0003] Plant hormones are closely related to cell division and elongation, tissue and organ differentiation, flowering and fruiting, maturation and senescence, dormancy and germination, and in vitro tissue culture, and regulate plant growth, development and differentiation separately or in coordination with each other. The flexibility and diversity of this regulation can be achieved by changing...

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Problems solved by technology

GC-MS has become one of the conventional methods for the analysis of plant endogenous hormones due to its advantages of rapid analysis, high sensitivity, and simultaneous qualitative and quantitative analysis. development of

[0006] Compared with GC-MS, ultra-high performance liquid chromatography tandem mass spectrometry has made great progress in speed, sensitivity and resolution. It is widely used in food, traditional Chinese medicine and other fields, but there are few reports on the analysis of plant endogenous hormones. Phase chromatography tandem mass spectrometry has a higher analytical throughput for trace components with complex matrices, but it still needs to be based on reliable analysis samples. Plant endogenous hormones are thermally unstable and plant components are complex. How to quickly and It is still a difficult problem to effectively obtain analysis samples, establish ideal analysis conditions, and reliably perform qualitative and quantitative detection of plant endogenous hormones.

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