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Rotary type biological sensor detection device and method

A technology of biosensors and detection devices, applied in the field of biosensors, can solve the problems of limited injection volume of samples to be tested, no products seen, time-consuming and laborious, etc., to reduce manpower and material costs, speed up binding time, and increase sensitivity Effect

Active Publication Date: 2013-10-23
HANGZHOU UMOTOR BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the research work in the world is still at the laboratory level. Although there are patent reports, no actual products have been seen.
[0005] In the past, when molecular motors were used for detection, they often relied on traditional containers, such as EP tubes, and performed many steps such as adding samples, static binding, washing, centrifuging, and removing waste liquid through a pipette gun. Separate implementation is not only time-consuming and laborious, but also limited by the capacity of EP tubes and pipette guns, often making the injection volume of samples to be tested limited, which also leads to the low sensitivity of existing detection products to a certain extent.

Method used

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  • Rotary type biological sensor detection device and method
  • Rotary type biological sensor detection device and method
  • Rotary type biological sensor detection device and method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Preparation of Rotary Biosensor

[0032] 1. Preparation of homogeneous chromophore

[0033] Inoculate the liquid culture medium (see appendix) from thermummicrobium roseum (ATCC27502) bacteria at a ratio of 1:100, culture at 60°C, 150rpm, for 24h. Then 4000rpm, 30min, 4 ℃ centrifugation to collect bacteria. With extraction Buffer (20mM Tris-Cl pH8.0, 100mM NaCl, 2mM MgCl 2 , 1 mM DTT) to resuspend the cells, and centrifuge to remove the supernatant (6000 rpm, 10 min, 4°C). Add extraction Buffer to resuspend the bacteria (approximately 1 g in 10 ml Buffer), then add a final concentration of 1 mM PMSF, and sonicate for 30 min on ice (5 s of sonication, 8 s off). Centrifuge the broken cells (25,000g, 30min, 4°C), remove the precipitate and get the supernatant. The supernatant was ultracentrifuged (145,000g, 1h, 4°C), and the precipitate was taken as Chromatophore. Prepare the gradient sucrose of five kinds of density again: 20% (2ml), 40% (2ml), 50% (2ml)...

Embodiment 2

[0065] Embodiment 2 Rotary biosensor detection device

[0066] A rotary biosensor detection device, such as Figure 1-3 As shown, the device is composed of a container cup, a sample adding tank 4 and a base 6, the sample adding tank 4 is arranged on the container container, the container container is arranged on the base 6, the bottom of the sample adding container 4 is provided with a buckle 5, and the container container It is composed of an outer cup 1, an inner cup 2 and a nitrocellulose film 3 laid on the bottom of the outer cup 1, and the buckle 5 is connected with the mouth of the inner cup 2. The base 6 is an 8-row base that accommodates 8 container cups. The row base adopts a parallel structure or a series structure, the series structure is a total sample outlet, the parallel structure is 8 sample outlets 7, and each container cup corresponds to a sample outlet 7.

Embodiment 3

[0067] Embodiment 3 detection method

[0068] Dilute the assembled molecular motor connected with the specific antibody with Tricine-HCl buffer solution at pH 6.0 according to the concentration required, and add sol-gel to the diluted solution at a ratio of 49:1. Add the prepared molecular motor solution with specific antibody evenly into the container cups covered with nitrocellulose film, add 50ul of the mixed solution into each container cup, after overnight in the refrigerator at 4°C, place in the drying box for 3 hours at room temperature , until completely dry.

[0069] Fix the sample tank on the prepared container covered with molecular motors, add the sample to be tested in the sample tank, after the antigen-antibody binding, wash with PBS, wash three times to wash away impurities and avoid non-specific binding. Among them, by adjusting or even closing the pore size of the sample outflow port or using a peristaltic pump, the flow rate can be effectively accelerated or...

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Abstract

The invention discloses a rotary type biological sensor detection device and a method which belong to the technical field of biological sensors. The device comprises a containing cup, a loading slot and a base; the loading slot is arranged on the containing cup, the containing cup is arranged on the base, the bottom of the loading slot is provided with a buckle, the containing cup comprises an outer cup, an inner cup and a nitrocellulose film laid on the bottom of the outer cup, and the buckle is connected with the mouth of the inner cup. The rotary type biological sensor detection device and the method have high speed, and can simply and significantly improve sensitivity and specificity of detection of chemical and biological materials by the rotary type biological sensor; and a series or parallel sample introduction idea and a corresponding sample introduction device are proposed for the first time, with the use of the idea and the device, the detection of a plurality of samples or a plurality of species can be carried out at the same time, so convenience is provided for detecting a large number of samples.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to a rotary biosensor detection device and method. Background technique [0002] At present, the research on rapid detection technology of chemical and biological samples has become one of the most important fields of international high technology. Rapid detection technology not only requires fast enough determination, but also requires sensitivity, convenience and high specificity. Therefore, in recent years, the intelligent sensor assembled with molecules, that is, the technology that integrates molecular recognition, signal conversion and measurement technology, has become a research focus. [0003] Biosensors can be divided into static and dynamic types. The research work of connecting tiny particles with biomolecules and constructing biochips (such as enzymes, nucleic acids, and biochrome complexes) with immobilized materials as carriers is called static type. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
Inventor 高颖魏建良
Owner HANGZHOU UMOTOR BIOTECH CO LTD
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