Functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as preparation method and application thereof

An ionic liquid and composite material technology is applied in the field of functionalized ionic liquid-hydrotalcite-like composite immobilized protein modified electrodes to achieve the effects of simple operation, high sensitivity and low preparation cost

Active Publication Date: 2013-11-27
佛山市顺德区德维医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the research on functionalized ionic liquid-inorganic nanocomposite modified electrodes is relatively active, most of them directly physically mix functionalized ionic liquids, inorganic nanomaterials and biological enzymes to...

Method used

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  • Functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as preparation method and application thereof
  • Functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as preparation method and application thereof
  • Functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0036] Divalent metal salt Zn(NO 3 ) 2 and trivalent metal salt Al(NO 3 ) 3 Prepare a mixed salt solution at a certain molar ratio of 2:1, the total metal ion concentration is 0.6 mol / L, add Hb to the mixed salt solution at a mass ratio of Hb:LDH of 0.05, and then add IL OH According to its combination with divalent metal salt Zn(NO 3 ) 2 The molar ratio of 1:2 is added to the mixed salt solution, and the above mixed salt solution is titrated with the sodium hydroxide solution with a molar concentration of 2 mol / L at the same time, and the pH is controlled between 9.0 and 10.0. Aging for 18 hours; the obtained slurry was suction filtered, the filter cake was washed 5 times with distilled water, and dried at room temperature for 24 hours to obtain the composite material Hb-IL loaded with Hb OH -LDH; The obtained composite material Hb-IL loaded with hemoglobin OH -LDH was prepared as a modification solution with a Hb content of 20 μg / μL, and 7 μL was drip-coated with 1 mol...

Embodiment 2

[0038] Divalent metal salt Zn(NO 3 ) 2 and trivalent metal salt Al(NO 3 ) 3 Prepare a mixed salt solution at a certain molar ratio of 2:1, the total metal ion concentration is 0.6 mol / L, add Hb to the mixed salt solution at a mass ratio of Hb:LDH of 0.25, and then add IL OH According to its combination with divalent metal salt Zn(NO 3 ) 2 The molar ratio of 1:2 is added to the mixed salt solution, and the above mixed salt solution is titrated with the sodium hydroxide solution with a molar concentration of 2 mol / L at the same time, and the pH is controlled between 9.0 and 10.0. Aging for 20 hours; the obtained slurry was suction filtered, the filter cake was washed 5 times with distilled water, and dried at room temperature for 24 hours to obtain the composite material Hb-IL loaded with Hb OH -LDH; The obtained composite material Hb-IL loaded with hemoglobin OH -LDH was prepared as a modification solution with a Hb content of 20 μg / μL, and 7 μL was drip-coated with 1 mol...

Embodiment 3

[0040] Divalent metal salt Zn(NO 3 ) 2 and trivalent metal salt Al(NO 3 ) 3 Prepare a mixed salt solution at a certain molar ratio of 2:1, the total metal ion concentration is 0.6 mol / L, add Hb to the mixed salt solution at a mass ratio of Hb:LDH of 0.5, and then add IL OH According to its combination with divalent metal salt Zn(NO 3 ) 2 The molar ratio of 1:2 is added to the mixed salt solution, and the above mixed salt solution is titrated with the sodium hydroxide solution with a molar concentration of 2 mol / L at the same time, and the pH is controlled between 9.0 and 10.0. Aging for 22 hours; the resulting slurry was suction filtered, the filter cake was washed 5 times with distilled water, and dried at room temperature for 24 hours to obtain the composite material Hb-IL loaded with Hb OH -LDH; The obtained composite material Hb-IL loaded with hemoglobin OH -LDH was prepared as a modification solution with a Hb content of 20 μg / μL, and 7 μL was drip-coated with 1 mol...

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Abstract

The invention discloses a functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as a preparation method and an application thereof. According to the technical scheme provided by the invention, firstly the fixation of an ionic liquid-hydrotalcite-like composite material to hemoglobin is realized by utilizing a coprecipitation method, then obtained hemoglobin-functionalized ionic liquid-hydrotalcite-like composite material is prepared into a solution which is dropwise coated on the surface of a preprocessed glassy carbon electrode, and the functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode is obtained after natural drying. According to the obtained composite modified electrode, the superiorities of functionalized ionic liquid and layered inorganic materials in the aspect of electrode modified materials are adequately combined, so that the modified electrode has high electrical conductivity and good dispersibility and enzyme fixation capacity. A hydrogen peroxide electrochemical sensor based on the modified electrode has the advantages of simplicity in operation, mild condition, low detection limit, high sensitivity, wide linear range and the like.

Description

technical field [0001] The invention relates to a functionalized ionic liquid-hydrotalcite-like composite material immobilized protein modified electrode; the invention also relates to a preparation method of the modified electrode and its application in electrochemical sensing and detection. Background technique [0002] Direct electrochemical research on biomacromolecules such as redox proteins and enzymes can not only help people deeply understand the electron transfer mechanism of biological systems, but also provide important theoretical basis and technical support for the development of new biosensors and new biofuel cells. However, their active centers are usually buried deep, and it is difficult to undergo direct electrochemistry on the electrode surface, and they are easily detached and denatured when they are directly adsorbed on the bare electrode surface. Therefore, finding materials that can maintain enzyme activity and facilitate direct electron transfer has be...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 詹天荣侯万国贾思佳徐洁
Owner 佛山市顺德区德维医疗科技有限公司
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