Preparation method of barley extract fermented by lactobacillus and anti-tumor effect of barley extract fermented by lactobacillus
A technology of fermentation and extraction of lactic acid bacteria, which can be used in anti-tumor drugs, food preparation, medical preparations containing active ingredients, etc. It can solve the problems that anti-tumor extracts have not yet been patented or reported in literature, and achieve rich nutrition and functions , low production cost, easy quality control effect
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Embodiment 1
[0035] Weigh 20.0g of barley flour crushed through a 60-mesh sieve into a 500mL conical flask, add 1:6 water to it and mix evenly, first use 0.015% β-glucanase to hydrolyze at 40°C for 30min, then use 4 % of Lactobacillus plantarum ( Lactobacillus plantarum Dy-1) Ferment at 30°C for 48 hours. After the fermentation, centrifuge the FBE at 6000 rpm / min for 20 minutes at room temperature, collect the supernatant, and freeze-dry it for 24 hours to obtain FBE.
[0036] Weigh 0.5g of FBE and dissolve it in 50ml of deionized water, pass through a 0.22um filter membrane, and filter the filtrate according to the concentration of 0.125 mg / mL, 0.25 mg / mL, 0.5 mg / mL, 1.0 mg / mL, 2.0 mg / ml, 4.0 mg / ml were added to HT-29 tumor cells, and the cells were cultured for 24h, 48h, and 72h, and the inhibitory rate of FBE to colon cancer HT-29 cells was determined by MTT method. The results showed that the concentration of the extract was positively correlated with the inhibition rate of tumor ce...
Embodiment 2
[0038]The above-prepared FBE was dissolved in physiological saline, prepared to a concentration of 0.2 g / ml and 0.1 g / ml, and processed through a sterile membrane for use. Human HT-29 colon cancer cells were inoculated subcutaneously into the abdomen of 4-6 week-old BALB / c nude mice, and 2 days later, 2 g / kg / d, 1 g / kg / d, 2 g / kg / d+5-FU ( 25 mg / kg / d, i.p, 7 days) dose of FBE was administered to tumor-bearing nude mice for 30 consecutive days. 7 days) compared with the 5-FU group, and the anti-tumor effect of FBE was confirmed by the volume of transplanted tumor measured with a vernier caliper and the final tumor inhibition rate ( figure 2 ,3). The results showed that the tumor inhibition rate of 2g / kg / d+5-FU mixed dose group could reach 53.36%.
[0039] Determination of superoxide dismutase (SOD) in the liver of tumor-bearing nude mice with the kit ( Figure 5 ), glutathione-peroxidase (GSH-PX) ( Image 6 ) and the content of biomacromolecule oxidative damage malondialdehyd...
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