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Preparation method of layer-by-layer self-assembled double-modified liposome

A layer-by-layer self-assembly and double-modification technology, applied in the fields of medicine, food, and cosmetics, can solve problems such as drug leakage, particle flocculation, and easy particle size increase

Inactive Publication Date: 2013-12-25
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of liposomes to encapsulate nutrients, enzymes, food additives, food antibacterials and other food delivery systems has shown attractive prospects. However, liposomes, as a particle dispersion system, have particle flocculation and variable particle size during storage. Large, drug leakage and other issues

Method used

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  • Preparation method of layer-by-layer self-assembled double-modified liposome
  • Preparation method of layer-by-layer self-assembled double-modified liposome
  • Preparation method of layer-by-layer self-assembled double-modified liposome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 0.206g of lecithin, 0.034g of cholesterol, 0.062g of Tween-80 and 0.004g of vitamin E, completely dissolve in 10ml of absolute ethanol, and remove the absolute ethanol by vacuum rotation in a water bath at 40°C to form a uniform film. Add 30ml of phosphate buffered saline (PBS) with pH7.4 and a concentration of 0.05M to wash the membrane, and the uniform suspension formed is the thick liposome. The crude liposomes were added into DHPM, and subjected to microfluidization treatment twice under the condition of 120 MPa to prepare nano liposomes. The prepared nano-liposome is a relatively transparent light blue solution, and the liposome solution is added dropwise to 0.6% chitosan (CH) solution while stirring, and its pH is adjusted to 5.5. After stirring for 1 hour, let stand for 1 hour to obtain chitosan-liposome (CH-L). Then CH-L was added dropwise to 0.5% sodium alginate (AL) solution while stirring, the pH of the solution was adjusted to 5.5, stirred for 1 hour,...

Embodiment 2

[0027] Weigh 0.411g of lecithin, 0.069g of cholesterol, 0.123g of Tween-80 and 0.008g of vitamin E, completely dissolve in 20ml of absolute ethanol, and remove the absolute ethanol by vacuum rotation in a water bath at 40°C to form a uniform film. Add 60ml of phosphate buffered saline (PBS) with pH7.4 and a concentration of 0.05M to wash the membrane, and the uniform suspension formed is the thick liposome. The crude liposomes were added into DHPM, and subjected to microfluidization treatment twice under the condition of 120 MPa to prepare nano liposomes. The prepared nano-liposome is a relatively transparent light blue solution, and the liposome solution is added dropwise to 0.6% chitosan (CH) solution while stirring, and its pH is adjusted to 5.5. After stirring for 1 hour, let stand for 1 hour to obtain chitosan-liposome (CH-L). Then CH-L was added dropwise to 0.5% sodium alginate (AL) solution while stirring, the pH of the solution was adjusted to 5.5, stirred for 1 hour,...

Embodiment 3

[0029]Weigh 0.206g of lecithin, 0.034g of cholesterol, 0.054g of Tween-80 and 0.006g of vitamin E, completely dissolve in 10ml of absolute ethanol, and remove the absolute ethanol by vacuum rotation in a water bath at 40°C to form a uniform film. Add 30ml of phosphate buffered saline (PBS) with pH7.4 and a concentration of 0.05M to wash the membrane, and the uniform suspension formed is the thick liposome. The crude liposomes were added into DHPM, and subjected to microfluidization treatment twice under the condition of 120 MPa to prepare nano liposomes. The prepared nano-liposome is a relatively transparent light blue solution, and the liposome solution is added dropwise to 0.6% chitosan (CH) solution while stirring, and its pH is adjusted to 5.5. After stirring for 1 hour, let stand for 1 hour to obtain chitosan-liposome (CH-L). Then CH-L was added dropwise to 0.5% sodium alginate (AL) solution while stirring, the pH of the solution was adjusted to 5.5, stirred for 1 hour, ...

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Abstract

The invention discloses a preparation method of a layer-by-layer self-assembled double-modified liposome. The preparation method comprises following step: lecithin and cholesterol are taken as wall materials, and a nano liposome is prepared by thin-film dispersion-dynamic high pressure microfluidization method; and layer-by-layer self-assemble technique is employed for further treatment, wherein primary amino group on chitosan molecular chains is taken as positive charge, carboxyl on sodium alginate molecular chains is taken as negative charge, polyelectrolyte films are formed layer by layer by electrostatic interaction between the positive charge and the negative charge, and the polyelectrolyte films are assembled onto the surface of the nano liposome so as to obtain the sodium alginate and chitosan double-modified liposome. Average particle size of the double-modified liposome is 330.6+-37.3nm, zeta potential is -15.79+-0.697mV, and distribution coefficient is 0.65+-0.048. It is shown by the results of in vitro digestion experiment that in vitro digestion stability of the double-modified liposome is higher than that of unmodified liposome; and release rate of fluorescent substance calcein encapsuled by the double-modified liposome after 120min of digestion is just 38+-2%, and that of fluorescent substance calcein encapsuled by the unmodified liposome is 58+-2%.

Description

technical field [0001] The invention specifically relates to a method for preparing a layer-by-layer self-assembled double-modified liposome, which can be further applied to the fields of food, medicine, cosmetics and the like. Background technique [0002] Liposome is a closed vesicle formed by a phospholipid bilayer and contains an aqueous phase inside. It has the advantages of slow release, cell affinity, tissue compatibility and targeting, and has been successfully used in biomedicine , chemical agriculture and other fields. The use of liposomes to encapsulate nutrients, enzymes, food additives, food antibacterials and other food delivery systems has shown attractive prospects. However, liposomes, as a particle dispersion system, have particle flocculation and variable particle size during storage. Large, drug leakage and other issues. In recent years, in order to increase the stability of the liposome bilayer membrane structure and release in vivo and in vitro, the re...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/36A61K8/02A23L1/29A23L33/00
Inventor 刘伟刘珍刘玮琳邹立强刘成梅梁瑞红
Owner NANCHANG UNIV
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