Method for producing edible mushroom nutrient solution by fermentation method

A technology for edible fungi and nutrient solution, applied in the direction of organic fertilizers, etc., can solve the problems affecting the quality and yield of edible fungi, complex metabolites, single nutrient elements, etc., and achieve the effect of improving biotransformation rate, easy absorption and high utilization rate

Active Publication Date: 2013-12-25
JING JING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The edible fungus nutrient solution compounded with chemical fertilizers only contains a large number of elements and trace elements, etc., and the single nutritional element affects the quali...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Cultivation of Brevibacterium flavum: Inoculate the inoculum of Brevibacterium flavum at 2% mass fraction into the shake flask culture medium, shake the culture on a shaking table, the number of shaking is 110 times / min, the culture temperature is 30°C, and the culture period is 12h , cultivated until the OD value was 1.0 to obtain the Brevibacterium flavum bacterium liquid; the shake flask culture medium was 3.5% glucose, 2% corn steep liquor, 1% yeast extract, 0.54% molasses, 3% ammonium sulfate, dihydrogen phosphate Potassium 0.3%, potassium dihydrogen phosphate 0.5%, and the balance is water.

[0023] (2) Submerged fermentation: Inoculate the Brevibacterium flavum liquid with a mass fraction of 2% into the medium of a 70L fermenter for submerged fermentation to obtain a fermented liquid; 2% pulp, 1% yeast extract, 0.54% molasses, 4% ammonium sulfate, 0.3% dipotassium hydrogen phosphate, 0.5% potassium dihydrogen phosphate, and the balance is water; fermentation ...

Embodiment 2

[0026] (1) Cultivation of Brevibacterium flavum: Inoculate Brevibacterium flavum with an inoculation amount of 1% in the shake flask culture medium, and shake it on a shaking table, the number of shaking is 110 times / min, the culture temperature is 28°C, and the culture period is 24h , cultivated to OD value of 0.8; the bacterial liquid was inoculated into 800L primary seed tank culture medium with a mass fraction of 2% inoculum, cultured at 30°C, rotated at 100rpm, cultured for 18 hours, cultivated to OD value of 0.5 Afterwards; the bacterial solution was inoculated into a 5T secondary seed tank culture medium with an inoculation amount of 2% by mass fraction, cultured at a temperature of 30°C, a rotating speed of 125rpm, and a culture period of 12h, and cultivated to an OD value of 0.8 to obtain a Brevibacterium flavum bacterial fluid Shake flask, primary seed tank and secondary seed tank medium are glucose 2%, corn steep liquor 3%, yeast extract 0.75%, molasses 0.75%, ammoni...

Embodiment 3

[0030](1) Cultivation of Brevibacterium flavum: Inoculate Brevibacterium flavum with an inoculum amount of 5% by mass fraction into the shake flask culture medium, culture on a shaking table with shaking frequency 110 times / min, culture temperature 32°C, culture period 24h , cultivated to OD value of 0.8; the bacterial solution was inoculated into 800L first-class seed tank culture medium with a mass fraction of 1% inoculum, cultured at 28°C, rotated at 125rpm, cultured for 12 hours, and cultivated to an OD value of 0.7 Afterwards; the bacterial solution was inoculated into a 5T secondary seed tank culture medium with an inoculation amount of 5% by mass fraction and cultured at a culture temperature of 32° C., a rotation speed of 150 rpm, and a culture period of 24 hours. The OD value was cultivated to 1.0 to obtain a Brevibacterium flavum bacterial fluid Shake flask, primary seed tank and secondary seed tank medium are glucose 5%, corn steep liquor 3.5%, yeast extract 1.25%, m...

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Abstract

The invention discloses a method for producing an edible mushroom nutrient solution by a fermentation method, which relates to the technical field of an organic fertilizer. The method comprises the following steps: (1) culturing brevibacterium flavum, namely inoculating the brevibacterium flavum, which is taken as an original strain, to a shake flask culture medium for culturing so as to obtain a brevibacterium flavum solution; (2) carrying out submerged fermentation, namely inoculating the brevibacterium flavum solution into a fermentation tank culture medium to carry out the submerged fermentation so as to obtain a fermentation solution; and (3) treating the fermentation solution, namely separating and concentrating the fermentation solution to obtain the edible mushroom nutrient solution. The edible mushroom nutrient solution obtained by the method disclosed by the invention is rich in various nutrition elements including crude proteins, short-chain peptides, amino acids, vitamin B1, vitamin B2, vitamin C and the like and is easy to absorb and high in utilization rate; the mushroom growing period can be prolonged and the biotransformation efficiency is improved; the quality and the yield of edible mushrooms are improved obviously.

Description

technical field [0001] The invention relates to the technical field of organic fertilizers. Background technique [0002] Edible fungus is a large fungus that can be eaten by humans. The main components of the fungus used in the cultivation of edible fungi are: cornmeal, cottonseed hulls, corncobs, sawdust, etc. After the four tides of edible fungi, the nutrients are basically consumed. The mushrooms are exhausted, and the fruiting is slow, so they are generally discarded. The number of moisture out of the fungus stick is less, and the utilization rate is low. In order to produce more mushrooms, produce good mushrooms, and achieve the purpose of improving quality, increasing yield and benefit, it is often necessary to add various nutrient solutions. [0003] At present, the production of edible fungi nutrient solution is mainly divided into two types: chemical fertilizer compound and edible fungus fertilizer containing live bacteria. The edible fungus nutrient solution co...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 李斌水吴雷谢章清张仲良
Owner JING JING PHARMA
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