Chlorella strain for synchronously treating municipal sewage and implementing grease accumulation

A technology for simultaneous treatment and municipal sewage, applied in the field of chlorella, can solve the problems of poor decontamination ability, poor algal species stress resistance, difficult to degrade carbon source materials, etc., and achieve the effect of strong anti-pollution ability

Active Publication Date: 2013-12-25
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] The present invention aims to solve the problems encountered in the coupling process of microalgae biodiesel production system and municipal sewage treatment system, such as poor stress resistance of algae species, low content of algae cell lipids, poor decontamination ability, difficulty in degrading carbon source substances in sewage and other problems. The problem of adding exogenous nutrients to growth in sewage provides a strain of chlorella that simultaneously treats municipal sewage and achieves oil accumulation

Method used

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  • Chlorella strain for synchronously treating municipal sewage and implementing grease accumulation
  • Chlorella strain for synchronously treating municipal sewage and implementing grease accumulation
  • Chlorella strain for synchronously treating municipal sewage and implementing grease accumulation

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specific Embodiment approach 1

[0012] Specific implementation mode 1: In this implementation mode, a strain of Chlorella that simultaneously treats municipal sewage and realizes oil accumulation, which is Chlorella sp. PF2, is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the storage address is It is No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing. The preservation date is July 30, 2013, and the preservation number is CGMCC No.8014.

[0013] The chlorella (Chlorella sp.) PF2 of this embodiment is single-celled, spherical, usually ellipsoidal when young, and has a thin cell wall. Cell length and diameter are 4-12 μm. Cells are green, with goblet-shaped chromatophores, accounting for most of the cell, with a pyrenoid nucleus (eg figure 1 and figure 2 shown).

[0014] Chlorella sp. PF2 of this embodiment has strong anti-pollution ability, can use its carbon, nitrogen, phosphorus and other pollutants as nutrients to grow a...

specific Embodiment approach 2

[0016] Specific embodiment 2: Chlorella sp. PF2 in this embodiment is screened from lakes in the Bund Wetland Park, West Sun Island, Harbin, Heilongjiang. Filtering is done in the following steps:

[0017] (1) Carefully collect the planktonic microalgae in the water with a sterile beaker, and store the collected water samples in a transparent glass bottle filled with sterile medium, put the glass bottle into a thermos bottle with ice cubes, and then bring it into the laboratory Separation and purification were carried out within 24 hours.

[0018] (2) Take an appropriate amount of algae source and add it to the Erlenmeyer flask containing sterilized BG11 medium, add a small amount of glass beads at the same time, place it on a shaker and shake it for about 24 hours, take it out and let it stand for about 30 minutes, absorb 50mL of the middle liquid, add about 150mL Place the flask in a sterile BG11 culture medium in a constant temperature light incubator for culture, shake it...

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Abstract

The invention relates to Chlorella, particularly a Chlorella strain for synchronously treating municipal sewage and implementing grease accumulation. The invention aims to solve the problems of poor stress tolerance, low alga cell grease content, poor decontamination capacity, difficulty in degrading carbon source substances in sewage, and requirement of adding exogenous nutrient substances for growth in sewage in the coupling process of a microalga biodiesel production system and a municipal sewage treatment system. The Chlorella sp. PF2 is collected at China General Microbiological Culture Collection Center, the collection address is Beichenxi Road No.1 Yard 3, Chaoyang District, Beijing City, the collection date is July 30th, 2013, and the collection number is CGMCC No.8014. The Chlorella sp. PF2 has strong anti-pollution capacity, and can grow and reproduce in common nonsterile municipal sewage by using carbon, nitrogen, phosphorus and other pollutants as nutrient substances while accumulating grease.

Description

technical field [0001] The present invention relates to a kind of chlorella. Background technique [0002] Facing the increasingly severe challenges of fossil energy and environmental pollution, the use of microalgae to produce biodiesel has attracted more and more attention. This is because microalgae biodiesel is a renewable energy source with high energy conversion efficiency, high oil content and proliferation of microalgae. The advantage of speed. In addition, the production process of microalgae biodiesel does not increase greenhouse gas emissions, which is conducive to environmental protection. However, the high production cost of microalgae biodiesel limits its large-scale production and wide application, and nutrients such as carbon, nitrogen, and phosphorus sources required for the growth of microalgae are an important part of the production cost of microalgae biodiesel. Municipal sewage is rich in carbon, nitrogen and phosphorus pollutants, and the massive disch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C02F3/34C12R1/89
CPCY02E50/10
Inventor 于艳玲冯玉杰李姗姗张大伟
Owner HARBIN INST OF TECH
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